| Data: |
2025 |
| Descripció: |
32 pag. |
| Resum: |
Idiopathic Nephrotic Syndrome (INS) is a rare renal disease whose etiology remains unclear, highlighting the need to find reliable biomarkers. In this context, haptoglobin (Hp), an expressed protein from three functional genotypes, has been proposed as a candidate. Previously, Hp 2-2 has been associated with inflammatory processes, and INS has shown high levels of Hp, presenting dysbiosis. This study aimed to determine differences between nasopharynx and gut microbiome profiles and fecal metabolome comparing healthy controls (Ctrl) and INS patients, according to their clinical status (activity vs. remission) and their Hp genotype. The composition of microbial communities was assessed through the amplification and sequencing of the V3-V4 variable regions of the 16S rRNA gene. Fecal metabolite concentrations were quantified using H-NMR. Dysbiosis was not detected in any of the microbial profiles. However, taxonomic and functional composition showed differences between Ctrl vs INS and activity vs remission. The main evidence identified was higher hypoxanthine fecal concentration in patients with genotype Hp 1-2 (p<0. 05), compared to Hp 1-1. Negative correlations were observed between these concentrations and g_ Veillonella's relative abundance (r=0. 65, p=0. 001) and some inferred pathways related to virulence factors. Results suggest that significant differences exist in both metagenomic and metabolomic profiles comparing INS patients with Ctrl and the different clinical statuses that could be modulated by the Hp genotype. Nonetheless, it is necessary to replicate these methods in studies with a larger sample. |
| Drets: |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre i quan aquestes es distribueixin sota la mateixa llicència que regula l'obra original i es reconegui l'autoria.  |
| Llengua: |
Castellà |
| Titulació: |
Bioquímica, Biologia Molecular i Biomedicina [4313794] |
| Pla d'estudis: |
Màster Universitari en Bioquímica, Biologia Molecular i Biomedicina [1599] |
| Document: |
Treball de fi de postgrau |
| Matèria: |
Microbioma ;
Barrera Intestinal ;
Biomarcadors ;
Inflammació ;
Podocit ;
Biomarcadores ;
Inflamación ;
Podocito ;
Microbiome ;
Gut Barrier ;
Biomarkers ;
Inflammation ;
Podocyte |
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