RENEB interlaboratory comparison for biological dosimetry based on dicentric chromosome analysis and cobalt-60 exposures higher than 2.5 Gy
Bucher, Martin (Federal Office for Radiation Protection. Department of Effects and Risks of Lonising and Non-Ionising Radiation)
Endesfelder, David (Federal Office for Radiation Protection. Department of Effects and Risks of Lonising and Non-Ionising Radiation)
Pojtinger, Stefan (Physikalisch- Technische Bundesanstalt. Department for Dosimetry for Radiation Therapy and Diagnostic Radiology)
Baeyens, Ans (Ghent University. Department of Human Structure and Repair)
Barquinero, Joan Francesc (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Beinke, Christina (Bundeswehr Institute of Radiobiology)
Bobyk, Laure (French Armed Forces Biomedical Research Institute. Department of Radiation Biological Effects)
Gregoire, Eric (Institut de Radioprotection et de Sûreté Nucléaire)
Hristova, Rositsa (National Centre of Radiobiology and Radiation Protection. Radiobiology Department)
Martinez, Juan S. (Institut de Radioprotection et de Sûreté Nucléaire)
Meher, Prabodha Kumar (Stockholm University)
Milanová, Marcela (University of Defence)
Monteiro Gil, Octávia (Universidade de Lisboa)
Montoro, Alegria (Hospital Universitari i Politècnic La Fe (València))
Moquet, Jayne (UK Health Security Agency)
Moreno Domene, Mercedes (Hospital General Universitario Gregorio Marañón)
Prieto Rodríguez, María Jesús (Hospital General Universitario Gregorio Marañón)
Pujol Canadell, Mònica (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Sun, Mingzhu (UK Health Security Agency)
Terzoudi, Georgia I. (National Centre for Scientific Research Demokritos)
Tichy, Ales (University of Defence)
Triantopoulou, Sotiria (National Centre for Scientific Research Demokritos)
Valente, Marco (French Armed Forces Biomedical Research Institute)
Vral, Anne (Ghent University)
Wojcik, Andrzej (National Centre of Radiobiology and Radiation Protection (Sofia, Bulgària))
Oestreicher, Ursula (Federal Office for Radiation Protection)

Data:	2025
Resum:	In previous RENEB interlaboratory comparisons based on the manual scoring of dicentric chromosomes, a tendency for systematic overestimation for doses > 2. 5 Gy was found. However, these exercises included only very few doses in the high dose range, and they were heterogeneous in terms of radiation quality and evaluation mode, and comparable only to a limited extent. Here, this presumed deviation was explored by investigating three doses > 2. 5 Gy. Blood samples were irradiated (2. 56, 3. 41 and 4. 54 Gy) using a 60Co source and sent to 14 member laboratories of the RENEB network, which performed the dicentric chromosome assay (manual and/or semi-automatic scoring) and reported dose estimates. Most participants provided estimates that agreed very well with the physical reference doses and all provided dose estimates were in the correct clinical category (> 2 Gy). The previously observed tendency for a systematic bias across all laboratories was not confirmed. However, tendencies for systematic underestimation were detected for dose estimations for reference doses given in terms of absorbed dose to blood and for some participants, a laboratory-specific trend of systematic under- or overestimation was observed. The importance of regularly performed quality checks for a broad dose range became obvious to avoid misinterpretation of results.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Ionising radiation ; Biological dosimetry ; Dicentric chromosome ; Interlaboratory comparison ; Network ; Radiation accident ; Cytogenetics ; Chromosomes ; Diagnostic markers
Publicat a:	Scientific reports, Vol. 15 (February 2025) , art. 5485, ISSN 2045-2322

DOI: 10.1038/s41598-025-89966-2
PMID: 39952996

10 p, 2.2 MB

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