A near-telomere to telomere phased genome assembly and annotation for the Australian central bearded dragon Pogona vitticeps
Patel, Hardip (Australian National University)
Alreja, Kirat (Australian National University)
Reis, Andre L.M. (University of New South Wales (Sydney, Austràlia))
Chang, J King (University of New South Wales (Sydney, Austràlia))
Chew, Zahra A. (Australian National University)
Jung, Hyungtaek (Australian National University)
Deveson, Ira W. (Australian National University)
Ruiz-Herrera Moreno, Aurora (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Marin Gual, Laia (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Zhang, Xiuwen (Australian National Wildlife Collection (Canberra, Austràlia))
Lister, Nicholas C. (University of New South Wales (Sydney, Austràlia))
Whiteley, Sarah (University of Canberra)
Xiong, Lei (University of Canberra)
Dissanayake, Duminda S.B. (University of Canberra)
Waters, Paul D. (University of New South Wales (Sydney, Austràlia))
Georges, Arthur (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Data:	2025
Descripció:	19 pàg.
Resum:	Background The central bearded dragon (Pogona vitticeps) is widely distributed in central eastern Australia and adapts readily to captivity. Among other attributes, it is distinctive because it undergoes sex reversal from ZZ genotypic males to phenotypic females at high incubation temperatures. Here, we report an annotated near telomere-to-telomere phased assembly of the genome of a female ZW central bearded dragon. Results Genome assembly length is 1. 75 Gbp with a scaffold N50 of 266. 2 Mbp, N90 of 28. 1 Mbp, 26 gaps, and 42. 2% GC content. Most (99. 6%) of the reference assembly is scaffolded into 6 macrochromosomes and 10 microchromosomes, including the Z and W microchromosomes, corresponding to the karyotype. The genome assembly exceeds standard recommended by the Earth Biogenome Project (6CQ40): 0. 003% collapsed sequence, 0. 03% false expansions, 99. 8% k-mer completeness, 97. 9% complete single-copy BUSCO genes, and an average of 93. 5% of transcriptome data mappable back to the genome assembly. The mitochondrial genome (16,731 bp) and the model ribosomal DNA repeat unit (length 9. 5 Kbp) were assembled. Male vertebrate sex genes Amh and Amhr2 were discovered as copies in the small non-recombining region of the Z chromosome, absent from the W chromosome. This, coupled with the prior discovery of differential Z and W transcriptional isoform composition arising from pseudo-autosomal sex gene Nr5a1, suggests that complex interactions between these genes, their autosomal copies, and their resultant transcription factors and intermediaries determine sex in the bearded dragon. Conclusion This high-quality assembly will serve as a resource to enable and accelerate research into the unusual reproductive attributes of this species and for comparative studies across the Agamidae and reptiles more generally.
Ajuts:	Agencia Estatal de Investigación PID2020-112557GB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00122
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Agamidae ; AusARG ; Squamata ; Lizard ; Sex determination
Publicat a:	GigaScience, Vol. 14 (2025) , p. giaf085, ISSN 2047-217X

DOI: 10.1093/gigascience/giaf085
PMID: 40825569

19 p, 11.7 MB

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