Camelina oil as a source of polyunsaturated fatty acids for the production of human milk fat substitutes catalyzed by a heterologous Rhizopus oryzae lipase
Faustino, Ana Rita (Universidade de Lisboa. Instituto Superior de Agronomia)
Osório, Natália M. (Universidade de Lisboa. Instituto Superior de Agronomia)
Tecelão, Carla (Universidade de Lisboa. Instituto Superior de Agronomia)
Canet, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Ferreira-Dias, Suzana (Universidade de Lisboa. Instituto Superior de Agronomia)

Data:	2016
Resum:	This study aims to produce human milk fat substitutes (HMFS) rich in polyunsaturated fatty acids (PUFA), mainly the essential omega-3 linolenic acid, by acidolysis reaction between tripalmitin (PPP) and free fatty acids (FFA) from camelina oil, in stirred batch reactor and solvent-free media. The non-commercial heterologous Rhizopus oryzae lipase (rROL), immobilized on Lewatit VP OC 1600 or on Relizyme OD403/S, and the immobilized commercial lipase from Rhizomucor miehei (Lipozyme RM IM) were tested as biocatalysts. Both lipases are sn-1,3 selective. After 24 h reaction at 60°C, using biocatalyst loads of 5 wt% (in relation to tripalmitin), 48. 9, 43. 6, and 18. 3 mol% of fatty acid incorporation in triacylglycerols (TAG) were obtained with Lipozyme RM IM and rROL immobilized on Lewatit or on Relizyme, respectively. rROL immobilized on Lewatit was selected as biocatalyst for the acidolysis, as alternative to the commercial immobilized lipases. With this biocatalyst, the molar incorporation increased with its initial water activity (0. 55-0. 95). Modeling acidolysis catalyzed by rROL immobilized on Lewatit was performed by response surface methodology, as a function of temperature (58-72°C) and molar ratio FFA:PPP (1. 2:1-6. 8:1). The highest PPP consumption was achieved at 60°C with a molar ratio of 2:1. The yield of HMFS (new TAG) increased from 42. 6 wt% (5% biocatalyst load) to 52% with 8% load, after 24 h acidolysis. Practical applications: Camelina oil showed to be a good source of PUFA, mainly essential fatty acids, to incorporate in HMFS. After 24 h acidolysis under optimized conditions, catalyzed by the non-commercial sn-1,3 regioselective rROL immobilized on Lewatit VP OC 1600, the TAG fraction contains 67. 7 mol% of palmitic acid at position 2. These structured lipids rich in PUFA can be used in blends with 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) in order to mimic the human milk fat. The performance of this biocatalyst was comparable to that observed with Lipozyme RM IM. The replacement of high-cost commercial immobilized lipases by rROL immobilized on Lewatit may reduce the biocatalyst cost. In addition, since the best molar ratio FFA:PPP for rROL is 2:1, i. e. , the stoichiometric value for the acidolysis catalyzed by sn-1,3 regioselective lipases, the use of this biocatalyst will reduce downstream costs related with unconverted FFA recovery.
Ajuts:	Ministerio de Economía y Competitividad CTQ2013-42391-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-452
Nota:	Altres ajuts: Integrated Action Portugal-Spain E-29/11
Drets:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Acidolysis ; Camelina oil ; Human milk fat substitutes ; Polyunsaturated fatty acids ; Rhizopus oryzae lipase ; Structured lipids
Publicat a:	European Journal of Lipid Science and Technology, Vol. 118, Issue 4 (April 2016) , p. 532-544, ISSN 1438-9312

DOI: 10.1002/ejlt.201500003

Postprint 81 p, 1.5 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats