Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents
Koutinas, Michalis (Cyprus University of Technology. Department of Environmental Science and Technology)
Yiangou, Chrystalleni (Cyprus University of Technology. Department of Environmental Science and Technology)
Osório, Natália M. (Instituto Politécnico de Setúbal)
Ioannou, Katerina (Cyprus University of Technology. Department of Environmental Science and Technology)
Canet Morral, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Ferreira-Dias, Suzana (Universidade de Lisboa. Instituto Superior de Agronomia)

Data:	2018
Resum:	This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0. 01-0. 1 M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent's Log P increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.
Ajuts:	Agencia Estatal de Investigación CTQ2016-74959-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Ethyl lactate ; Esterification ; Lipase ; Novozym 435 ; Operational stability
Publicat a:	Bioresource Technology, Vol. 247 (January 2018) , p. 496-503, ISSN 1873-2976

DOI: 10.1016/j.biortech.2017.09.130
PMID: 28968571

Postprint 36 p, 955.5 KB

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