Secondary structure impacts patterns of selection in human lncRNAs
Pegueroles Queralt, Maria Cinta (Universitat Pompeu Fabra)
Gabaldón, Toni (Universitat Pompeu Fabra)

Data:	2016
Descripció:	13 pàg.
Resum:	Background: Metazoans transcribe many long non-coding RNAs (lncRNAs) that are poorly conserved and whose function remains unknown. This has raised the questions of what fraction of the predicted lncRNAs is actually functional, and whether selection can effectively constrain lncRNAs in species with small effective population sizes such as human populations. Results: Here we evaluate signatures of selection in human lncRNAs using inter-specific data and intra-specific comparisons from five major populations, as well as by assessing relationships between sequence variation and predictions of secondary structure. In all analyses we included a reference of functionally characterized lncRNAs. Altogether, our results show compelling evidence of recent purifying selection acting on both characterized and predicted lncRNAs. We found that RNA secondary structure constrains sequence variation in lncRNAs, so that polymorphisms are depleted in paired regions with low accessibility and tend to be neutral with respect to structural stability. Conclusions: Important implications of our results are that secondary structure plays a role in the functionality of lncRNAs, and that the set of predicted lncRNAs contains a large fraction of functional ones that may play key roles that remain to be discovered.
Ajuts:	Ministerio de Economía y Competitividad SEV-2012-0208 Ministerio de Economía y Competitividad BFU2015-67107 European Commission 606786 European Commission 310325 European Commission 642095
Nota:	Altres ajuts: SGR-857
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Divergence ; Polymorphism ; Purifying selection ; Secondary structure ; lncRNA
Publicat a:	BMC biology, Vol. 14, Num. 1 (December 2016) , art. 60, ISSN 1741-7007

DOI: 10.1186/s12915-016-0283-0
PMID: 27457204

13 p, 2.2 MB

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