Insights into the causal role of diesel exhaust particles in ventricular arrhythmogenesis : protective effects of antioxidant cerium oxide nanoparticles
Ganse, Freddy G (Vall d'Hebron Institut de Recerca (VHIR))
Ernst, Lena M. (Vall d'Hebron Institut de Recerca (VHIR))
Rodríguez Antona, Cristina (Instituto de Salud Carlos III)
Ruiz Meana, Marisol (Vall d'Hebron Institut de Recerca (VHIR))
Inserte, Javier (Vall d'Hebron Institut de Recerca (VHIR))
Martínez-González, José (Instituto de Salud Carlos III)
Briones, Ana (Universidad Autónoma de Madrid)
Consegal, Marta (Vall d'Hebron Institut de Recerca (VHIR))
Miró-Casas, Elisabet (Vall d'Hebron Institut de Recerca (VHIR))
Yáñez-Bisbe, Laia (Vall d'Hebron Institut de Recerca (VHIR))
Pomposo, Aitor (Vall d'Hebron Institut de Recerca (VHIR))
Prades, Marta (Vall d'Hebron Institut de Recerca (VHIR))
Ferreira-Gonzalez, Ignacio (Universitat Autònoma de Barcelona. Departament de medicina)
Puntes, Víctor (Vall d'Hebron Institut de Recerca (VHIR))
Benito Villabriga, Begoña (Universitat Autònoma de Barcelona. Departament de medicina)
Rodríguez Sinovas, Antonio (Vall d'Hebron Institut de Recerca (VHIR))

Data:	2025
Resum:	Epidemiological studies suggest an association between air pollution and ventricular arrhythmias, with reactive oxygen species (ROS) playing a crucial role. However, the causal relationship and long-term effects remain uncertain, and the effectiveness of interventions aimed at reducing ROS requires further investigation. Here we aimed to evaluate the effects of a 3-weeks exposure to diesel exhaust particles (DEPs) on ventricular arrhythmogenesis, explore the underlying mechanisms, and assess the potential of cerium oxide nanoparticles (CeONP) as a ROS-detoxifying intervention. Sprague-Dawley rats underwent intratracheal instillation of saline without or with DEPs (7. 5 g/Kg for 1-3 weeks). Ventricular arrhythmia inducibility was then assessed in isolated hearts using a protocol of programmed electrical stimulation. Cardiac hypertrophy, collagen content, inflammation and oxidative stress were analyzed using histology, Western blot, RT-qPCR, and measurement of malondialdehyde content. The potential protective effects of CeONP (0. 5 mg/Kg/week, i. p. ) were also tested. DEP exposure for 3 weeks increased the incidence and duration of sustained ventricular tachyarrhythmias (VTs), a finding that correlated with a moderate increase in interstitial collagen (from 3. 11 ± 0. 12% in controls to 4. 80 ± 0. 21% in DEP-exposed rats, p. < 0. 001), and an early upregulation in the expression of collagen and other fibrotic and inflammatory markers. These effects associated with prolonged QRS complex and enhanced malondialdehyde content (356. 7 ± 21. 2 vs. 455. 3 ± 17. 2 μmol/g tissue, p = 0. 0066) after 3 weeks. CeO2NP treatment reduced oxidative stress and myocardial fibrosis, reversed electrocardiographic changes and attenuated DEP-induced pro-arrhythmic effects. Conclusions: DEP exposure increases the incidence and duration of sustained VTs, collagen deposition and oxidative stress in rats. Treatment with CeO2NP attenuate these effects, arising as a potential novel strategy to mitigate the deleterious effects of air pollution.
Ajuts:	Instituto de Salud Carlos III PI20/01649 Generalitat de Catalunya 2021/SGR-00758
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Air pollution ; Ventricular arrhythmias ; Oxidative stress ; Fibrosis ; Cerium oxide
Publicat a:	Particle and fibre toxicology, Vol. 22, Num. 1 (December 2025) , art. 36, ISSN 1743-8977

DOI: 10.1186/s12989-025-00649-2
PMID: 41444986

16 p, 3.7 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats