Articles

Articles 17 records found  previous11 - 17  jump to record: Search took 0.17 seconds. 
11.
27 p, 985.0 KB Targeting low-density lipoprotein receptors with protein-only nanoparticles / Xu, Zhikun (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Céspedes, María Virtudes (Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina) ; Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Álamo, Patricia (Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina) ; Pesarrodona Roches, Mireia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Mangues, Ramón (Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina) ; Vázquez Gómez, Esther, dir. (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Low-density lipoprotein receptors (LDLR) are appealing cell surface targets in drug delivery, as they are expressed in the blood–brain barrier (BBB) endothelium and are able to mediate transcytosis of functionalized drugs for molecular therapies of the central nervous system (CNS). [...]
2015 - 10.1007/s11051-015-2959-8
Journal of nanoparticle research, Vol. 17 issue 3 (March 2015) , article 150  
12.
15 p, 3.4 MB Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins / Mamat, Uwe (Forschungszentrum Borstel) ; Wilke, Kathleen (Forschungszentrum Borstel) ; Bramhill, David (Research Corporation Technologies (Tucson)) ; Schromm, Andra Beate (Forschungszentrum Borstel) ; Lindner, Buko (Forschungszentrum Borstel) ; Kohl, Thomas Andreas (Forschungszentrum Borstel) ; Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Schaffer, Lana (Scripps Research Institute) ; Head, Steven Robert (Scripps Research Institute) ; Souvignier, Chad (Research Corporation Technologies (Tucson)) ; Meredith, Timothy Charles (Pennsylvania State University. Department of Biochemistry and Molecular Biology) ; Woodard, Ronald Wesley (Bramhill Biological Consulting)
Background: lipopolysaccharide (LPS), also referred to as endotoxin, is the major constituent of the outer leaflet of the outer membrane of virtually all Gram-negative bacteria. The lipid A moiety, which anchors the LPS molecule to the outer membrane, acts as a potent agonist for Toll-like receptor 4/myeloid differentiation factor 2-mediated pro-inflammatory activity in mammals and, thus, represents the endotoxic principle of LPS. [...]
2015 - 10.1186/s12934-015-0241-5
Microbial cell factories, Vol. 14, Issue 57 (2015)  
13.
18 p, 135.9 KB Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories : production of human alpha-galactosidase A. / Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Vázquez Lima, Felícitas (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Accardi, Giulia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Mendoza, Rosa (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Toledo-Rubio, Verónica (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Giuliani, Maria (Università degli Studi di Palermo. Dipartimento di Scienze Biochimiche) ; Sannino, Filomena (Università degli Studi di Palermo. Dipartimento di Scienze Biochimiche) ; Parrilli, Ermenegilda (Università degli Studi di Palermo. Dipartimento di Scienze Biochimiche) ; Abasolo, Ibane (Vall d'Hebron Institut de Recerca) ; Schwartz, Simo (Vall d'Hebron Institut de Recerca) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Tutino, Maria L. (Università degli Studi di Palermo. Dipartimento di Scienze Biochimiche)
Obtaining high levels of pure proteins remains the main bottleneck of many scientific and biotechnological studies. Among all the available recombinant expression systems, Escherichia coli facilitates gene expression by its relative simplicity, inexpensive and fast cultivation, well-known genetics and the large number of tools available for its biotechnological application. [...]
2015 - 10.1007/s00253-014-6328-9
Applied microbiology and biotechnology, Vol 99, Issue 14 (July 2015) , p. 5863-5874
3 documents
14.
7 p, 774.5 KB Expanding the recombinant protein quality in Lactococcus lactis / Cano Garrido, Olivia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Rueda, Fabián L. (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Sànchez-García, Laura (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ruiz-Ávila, Luis (Spherium Biomed S.L.) ; Bosser, Ramon (Spherium Biomed S.L.) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; García Fruitós, Elena (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Background: Escherichia coli has been a main host for the production of recombinant proteins of biomedical interest, but conformational stress responses impose severe bottlenecks that impair the production of soluble, proteolytically stable versions of many protein species. [...]
2014 - 10.1186/s12934-014-0167-3
Microbial cell factories, Vol. 13 (2014) , p. 167  
15.
18 p, 391.8 KB Production of functional inclusion bodies in endotoxin-free Escherichia coli / Rueda, Fabián (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Mamat, Uwe (Forschungszentrum Borstel) ; Wilke, Kathleen (Forschungszentrum Borstel) ; Seras Franzoso, Joaquin (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; García Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). [...]
2014 - 10.1007/s00253-014-6008-9
Applied microbiology and biotechnology, Vol. 98 issue 22 (Nov. 2014) , p. 9229-9238
2 documents
16.
10 p, 638.7 KB Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions / Carnicer Heras, Marc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Ten Pierick, Angela (Delft University of Technology. Department of Biotechnology (Delft, Països Baixos)) ; van Dam, Jan (Delft University of Technology. Department of Biotechnology (Delft, Països Baixos)) ; Heijnen, Joseph J. (Delft University of Technology. Department of Biotechnology (Delft, Països Baixos)) ; Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; van Gulik, Walter (Delft University of Technology. Department of Biotechnology (Delft, Països Baixos)) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Background: Environmental and intrinsic stress factors can result in the global alteration of yeast physiology, as evidenced by several transcriptional studies. Hypoxia has been shown to have a beneficial effect on the expression of recombinant proteins in Pichia pastoris growing on glucose. [...]
2012 - 10.1186/1475-2859-11-83
Microbial cell factories, Vol. 11, N. 83 (June 2012) , p. 1-10
4 documents
17.
9 p, 664.7 KB Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures / Fernández Castañé, Alfred (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Caminal i Saperas, Glòria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; López Santín, Josep (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Background: The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production. However, lac operon dynamics and induction behavior remain the paradigm of gene regulation. [...]
2012 - 10.1186/1475-2859-11-58
Microbial cell factories, Vol. 11, N. 58 (May 2012) , p. 1-9
2 documents

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