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Articles 39 records found  beginprevious16 - 25nextend  jump to record: Search took 0.01 seconds. 
16.
13 p, 416.2 KB Rationale-based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris) / Nieto-Taype, Miguel Angel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Garrigos, Javier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Sánchez-Farrando, Marc (Purac Bioquímica S.A.) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Its features as a microbial and eukaryotic organism have turned Komagataella phaffii (Pichia pastoris) into an emerging cell factory for recombinant protein production (RPP). As a key step of the bioprocess development, this work aimed to demonstrate the importance of tailor designing the cultivation strategy according to the production kinetics of the cell factory. [...]
2020 - 10.1111/1751-7915.13498
Microbial biotechnology, Vol. 13, Issue 2 (Març 2020) , p. 315-327  
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17.
50 p, 2.4 MB Rhizopus oryzae lipase, a promising industrial enzyme : biochemical characteristics, production and biocatalytic applications / López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Lipases are biocatalysts with a significant potential to enable a shift from current pollutant manufacturing processes to environmentally sustainable approaches. The main reason of this prospect is their catalytic versatility as they carry out several industrially relevant reactions as hydrolysis of fats in water/lipid interface and synthesis reactions in solvent-free or non-aqueous media such as transesterification, interesterification and esterification. [...]
2020 - 10.3390/catal10111277
Catalysts, Vol. 10, Issue 11 (November 2020) , art. 1277  
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18.
2 p, 329.6 KB Intel·ligència artificial per al control de bioprocessos fermentatius / Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Investigadors del Departament d'Enginyeria Química, Biològica i Ambiental de la UAB participen en el projecte internacional Continued Process Validation (CPV) of the Future per aplicar la intel·ligència artificial en el control de bioprocessos fermentatius.
Investigadores del Departamento de Ingeniería Química, Biológica y Ambiental de la UAB participan en el proyecto internacional Continued Process Validation (CPV) of the Future para aplicar la inteligencia artificial en el control de bioprocesos fermentativos.
Researchers from the Department of Chemical, Biological and Environmental Engineering of the UAB are collaborating in the international project "Continued Process Validation (CPV) of the Future" in order to apply artificial intelligence for fermentative bioprocess control.
2020
UAB divulga, Setembre 2020
3 documents
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19.
15 p, 1.6 MB Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) P-driven recombinant producer strains with different target gene dosage / Garrigos, Javier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Nieto-Taype, Miguel Angel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Gasset Franch, Arnau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The P-based expression system is the most widely used for producing recombinant proteins in the methylotrophic yeast Pichia pastoris (Komagataella phaffii). Despite relevant recent advances in regulation of the methanol utilization (MUT) pathway have been made, the role of specific growth rate (µ) in AOX1 regulation remains unknown, and therefore, its impact on protein production kinetics is still unclear. [...]
2019 - 10.1186/s12934-019-1240-8
Microbial cell factories, Vol. 18 (November 2019) , art. 187  
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20.
16 p, 2.6 MB Truncated prosequence of rhizopus oryzae lipase : key factor for production improvement and biocatalyst stability / López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Barrero Peña, Juan José (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Recombinant Rhizopus oryzae lipase (mature sequence, rROL) was modified by adding to its N-terminal 28 additional amino acids from the C-terminal of the prosequence (proROL) to obtain a biocatalyst more suitable for the biodiesel industry. [...]
2019 - 10.3390/catal9110961
Catalysts, Vol. 9, issue 11 (Nov. 2019) , art. 961  
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21.
38 p, 722.7 KB The potential use of lipases in the production of fatty acid derivatives for the food and nutraceutical industries / Ferreira-Dias, Suzana (Universidade Técnica de Lisboa. Instituto Superior de Agronomia) ; Sandoval, Georgina (Centro de Investigación y Asistencia en Tecnologia y Diseño del Estado de Jalisco) ; Plou, Francisco (Instituto de Catálisis y Petroleoquímica (Madrid)) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The potential of lipases (E. C. 3. 1. 1. 3. ) as biocatalysts for the production of fatty acid derivatives for the food and nutraceutical industries, such as flavouring esters, fatty acid esters of antioxidants and structured lipids, is enormous, mainly due to their high regio- and stereo-selectivities, in addition to the other well-known advantages of enzymatic processes. [...]
2013 - 10.2225/vol16-issue3-fulltext-5
Electronic journal of biotechnology, Vol. 16, issue 3 (2013)  
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22.
9 p, 1.7 MB Overall key performance indicator to optimizing operation of high-pressure homogenizers for a reliable quantification of intracellular components in Pichia pastoris / Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Reyes León, Cecilia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The most commonly used cell disruption procedures may present lack of reproducibility, which introduces significant errors in the quantification of intracellular components. In this work, an approach consisting in the definition of an overall key performance indicator (KPI) was implemented for a lab scale high-pressure homogenizer (HPH) in order to determine the disruption settings that allow the reliable quantification of a wide sort of intracellular components. [...]
2015 - 10.3389/fbioe.2015.00107
Frontiers in Bioengineering and Biotechnology, Vol. 3 (August 2015) , art. 107  
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23.
13 p, 1.4 MB An improved secretion signal enhances the secretion of model proteins from Pichia pastoris / Barrero Peña, Juan José (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Casler, Jason C. (University of Chicago. Department of Molecular Genetics and Cell Biology) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Glick, Benjamin S. (University of Chicago. Department of Molecular Genetics and Cell Biology)
Background: proteins can be secreted from a host organism with the aid of N-terminal secretion signals. The budding yeast Pichia pastoris (Komagataella sp. ) is widely employed to secrete proteins of academic and industrial interest. [...]
2018 - 10.1186/s12934-018-1009-5
Microbial cell factories, Vol. 17 (2018) , art. 161  
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24.
28 p, 709.9 KB Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis / Bonet Ragel, Kírian (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Canet Morral, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background: using renewable feedstock sources for biodiesel production seems to be a promising strategy and even more so when enzymatic catalysis with lipases are used. However, it is well known that these enzymes could be inactivated by reaction conditions such as temperature or alcohol concentration. [...]
2018 - 10.1002/jctb.5399
Journal of chemical technology and biotechnology, Vol. 93, issue 2 (Feb. 2018) , p. 541-547  
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25.
84 p, 1.1 MB Enzyme-catalyzed production of biodiesel as alternative to chemical-catalyzed processes : advantages and constraints / Sandoval, Georgina (Centro de Investigación y Asistencia en Tecnologia y Diseño del Estado de Jalisco) ; Casas-Godoy, Leticia (Centro de Investigación y Asistencia en Tecnologia y Diseño del Estado de Jalisco) ; Bonet Ragel, Kírian (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Rodrigues, Joana (Universidade de Lisboa. Instituto Superior de Agronomia) ; Ferreira-Dias, Suzana (Universidade de Lisboa. Instituto Superior de Agronomia) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background: Biodiesel represents an interesting alternative to fossil fuels. Traditionally the standard method for biodiesel production from oils is alkaline-catalyzed transesterification. Chemical catalysis can be replaced by enzymatic catalysis using lipases (EC 3. [...]
2017 - 10.2174/2212711904666170615123640
Current biochemical engineering, Vol. 4, issue 2 (2017) , p. 109-141  
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