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16 p, 1.8 MB Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris / Cámara, Elena (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Landes, Nils (University of Natural Resources and Life Sciences Vienna) ; Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Gasser, Brigitte (Universität für Bodenkultur Wien) ; Mattanovich, Diethard (Universität für Bodenkultur Wien) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The methanol-regulated alcohol oxidase promoter (PAOX1) of Pichia pastoris is one of the strongest promoters for heterologous gene expression in this methylotrophic yeast. Although increasing gene dosage is one of the most common strategies to increase recombinant protein productivities, the increase of gene dosage of Rhizopus oryzae lipase (ROL) in P. [...]
2017 - 10.1038/srep44302
Scientific reports, Vol. 7 (2017) , art. 44302  
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