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19 p, 3.7 MB Nuclear RNA purification by flow cytometry to study nuclear processes in plants / Moro, Belen (Centre de Recerca en Agrigenòmica) ; Kisielow, Malgorzata (Zurich Flow Cytomtery Core Facility) ; Barragan Borrero, Veronica (Gregor Mendel Institute of Molecular Plant Biology) ; Bouet, Antoine (Centre de Recerca en Agrigenòmica) ; Brosnan, Christopher (University of Queensland, Brisbane) ; Bologna, Nicolás (Centre de Recerca en Agrigenòmica)
The nature of plant tissues has continuously hampered understanding of the spatio-temporal and subcellular distribution of RNA-guided processes. Here, we describe a universal protocol based on Arabidopsis to investigate subcellular RNA distribution from virtually any plant species using flow cytometry sorting. [...]
2021 - 10.1016/j.xpro.2021.100320
STAR Protocols, Vol. 2, Issue 1 (March 2021) , art. 100320  

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