Results overview: Found 3 records in 0.01 seconds.
Articles, 3 records found
Articles 3 records found  
1.
16 p, 2.6 MB Truncated prosequence of rhizopus oryzae lipase : key factor for production improvement and biocatalyst stability / López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Barrero, Juan J. (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Recombinant Rhizopus oryzae lipase (mature sequence, rROL) was modified by adding to its N-terminal 28 additional amino acids from the C-terminal of the prosequence (proROL) to obtain a biocatalyst more suitable for the biodiesel industry. [...]
2019 - 10.3390/catal9110961
Catalysts, Vol. 9, issue 11 (Nov. 2019) , art. 961  
2.
13 p, 1.4 MB An improved secretion signal enhances the secretion of model proteins from Pichia pastoris / Barrero, Juan J. (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Casler, Jason C. (University of Chicago. Department of Molecular Genetics and Cell Biology) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Quimica, Biològica i Ambiental) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Glick, Benjamin S. (University of Chicago. Department of Molecular Genetics and Cell Biology)
Background: proteins can be secreted from a host organism with the aid of N-terminal secretion signals. The budding yeast Pichia pastoris (Komagataella sp. ) is widely employed to secrete proteins of academic and industrial interest. [...]
2018 - 10.1186/s12934-018-1009-5
Microbial cell factories, Vol. 17 (2018) , art. 161  
3.
14 p, 1.6 MB Golden Pi CS : a golden gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris / Prielhofer, Roland (Austrian Centre of Industrial Biotechnology) ; Barrero, Juan J. (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Steuer, Stefanie (Universität für Bodenkultur Wien) ; Gassler, Thomas (Universität für Bodenkultur Wien) ; Zahrl, Richard (Universität für Bodenkultur Wien) ; Baumann, Kristin (Universität für Bodenkultur Wien) ; Sauer, Michael (Universität für Bodenkultur Wien) ; Mattanovich, Diethard (Universität für Bodenkultur Wien) ; Gasser, Brigitte (Universität für Bodenkultur Wien) ; a Marx, Hans (Universität für Bodenkultur Wien)
State-of-the-art strain engineering techniques for the host Pichia pastoris (syn. Komagataella spp. ) include overexpression of homologous and heterologous genes, and deletion of host genes. For metabolic and cell engineering purposes the simultaneous overexpression of more than one gene would often be required. [...]
2017 - 10.1186/s12918-017-0492-3
BMC systems biology, Vol. 11 (Dec. 2017) , art. 123  

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