1.
|
18 p, 2.7 MB |
Partial restoration of immune response in Hepatitis C patients after viral clearance by direct-acting antiviral therapy
/
Llorens-Revull, Meritxell (Universitat Autònoma de Barcelona) ;
Costafreda, Maria Isabel (Banc de Sang i Teixits (Barcelona, Catalunya)) ;
Rico, Angie (Banc de Sang i Teixits (Barcelona, Catalunya)) ;
Guerrero-Murillo, Mercedes (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Soria, Maria Eugenia (Universidad Autónoma de Madrid) ;
Píriz-Ruzo, Sofía (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Vargas-Accarino, Elena (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Gabriel-Medina, Pablo (Hospital Universitari Vall d'Hebron) ;
Rodríguez-Frías, Francisco (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Riveiro Barciela, Mar (Hospital Universitari Vall d'Hebron) ;
Perales Viejo, Celia (Universidad Autónoma de Madrid) ;
Quer, Josep 1963- (Universitat Autònoma de Barcelona) ;
Sauleda, Silvia (Banc de Sang i Teixits (Barcelona, Catalunya)) ;
Esteban Mur, Juan Ignacio (Hospital Universitari Vall d'Hebron) ;
Bes, Marta (Banc de Sang i Teixits (Barcelona, Catalunya))
HCV CD4+ and CD8+ specific T cells responses are functionally impaired during chronic hepatitis C infection. DAAs therapies eradicate HCV infection in more than 95% of treated patients. However, the impact of HCV elimination on immune responses remain controversial. [...]
2021 - 10.1371/journal.pone.0254243
PloS one, Vol. 16 (july 2021)
|
|
2.
|
15 p, 703.1 KB |
Ultra-Deep Pyrosequencing Detects Conserved Genomic Sites and Quantifies Linkage of Drug-Resistant Amino Acid Changes in the Hepatitis B Virus Genome
/
Rodriguez-Frías, Francisco (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Tabernero, David (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Quer, Josep 1963- (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Esteban Mur, Juan Ignacio (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Ortega, Israel (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Domingo, Esteban (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Cubero, María Dolores (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Camós Anguila, Sílvia (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Ferrer, Carles (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Sánchez, Alex (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Jardí, Rosendo (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Schaper, Melanie (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Homs, Maria (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Garcia-Cehic, D. (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Guardia, Jaume (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Esteban, Rafael (Esteban Mur) (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Buti, Maria (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Universitat Autònoma de Barcelona
Selection of amino acid substitutions associated with resistance to nucleos(t)ide-analog (NA) therapy in the hepatitis B virus (HBV) reverse transcriptase (RT) and their combination in a single viral genome complicates treatment of chronic HBV infection and may affect the overlapping surface coding region. [...]
2012 - 10.1371/journal.pone.0037874
PloS one, Vol. 7 (may 2012)
|
|
3.
|
13 p, 470.0 KB |
Ultra-Deep Pyrosequencing (UDPS) Data Treatment to Study Amplicon HCV Minor Variants
/
Gregori i Font, Josep (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Esteban Mur, Juan Ignacio (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Cubero, María Dolores (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Garcia-Cehic, D. (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Perales Viejo, Celia (Centro de Biología Molecular Severo Ochoa) ;
Casillas, Rosario (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Alvarez-Tejado, Miguel (Roche Diagnostics, Sant Cugat del Vallès) ;
Rodríguez-Frías, Francisco (Biochemistry Unit, HUVH, Barcelona, Spain) ;
Guardia, Jaume (Universitat Autònoma de Barcelona) ;
Domingo, Esteban (Centro de Biología Molecular Severo Ochoa) ;
Quer, Josep 1963- (Universitat Autònoma de Barcelona)
We have investigated the reliability and reproducibility of HCV viral quasispecies quantification by ultra-deep pyrosequencing (UDPS) methods. Our study has been divided in two parts. First of all, by UDPS sequencing of clone mixes samples we have established the global noise level of UDPS and fine tuned a data treatment workflow previously optimized for HBV sequence analysis. [...]
2013 - 10.1371/journal.pone.0083361
PloS one, Vol. 8 (december 2013)
|
|
4.
|
19 p, 2.2 MB |
Lethal Mutagenesis of Hepatitis C Virus Induced by Favipiravir
/
de Ávila, Ana Isabel (Consejo Superior de Investigaciones Científicas) ;
Gallego, Isabel (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) ;
Soria, Maria Eugenia (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Gregori i Font, Josep (Roche Diagnostics (Sant Cugat)) ;
Quer, Josep 1963- (Universitat Autònoma de Barcelona) ;
Esteban Mur, Juan Ignacio (Universitat Autònoma de Barcelona) ;
Rice, Charles M. (The Rockefeller University) ;
Domingo, Esteban (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) ;
Perales Viejo, Celia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Lethal mutagenesis is an antiviral approach that consists in extinguishing a virus by an excess of mutations acquired during replication in the presence of a mutagen. Here we show that favipiravir (T-705) is a potent mutagenic agent for hepatitis C virus (HCV) during its replication in human hepatoma cells. [...]
2016 - 10.1371/journal.pone.0164691
PloS one, Vol. 11 (october 2016)
|
|
5.
|
17 p, 3.0 MB |
Rare haplotype load as marker for lethal mutagenesis
/
Gregori i Font, Josep (Roche Diagnostics) ;
Soria, María Eugenia (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Gallego, Isabel (Centro de Biología Molecular Severo Ochoa) ;
Guerrero-Murillo, Mercedes (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Esteban Mur, Juan Ignacio (Universitat Autònoma de Barcelona) ;
Quer, Josep 1963- (Universitat Autònoma de Barcelona) ;
Perales Viejo, Celia (Centro de Biología Molecular Severo Ochoa) ;
Domingo, Esteban (Centro de Biología Molecular Severo Ochoa)
RNA viruses replicate with a template-copying fidelity, which lies close to an extinction threshold. Increases of mutation rate by nucleotide analogues can drive viruses towards extinction. This transition is the basis of an antiviral strategy termed lethal mutagenesis. [...]
2018 - 10.1371/journal.pone.0204877
PloS one, Vol. 13 (october 2018)
|
|
6.
|
15 p, 3.3 MB |
Pipeline for specific subtype amplification and drug resistance detection in hepatitis C virus
/
Soria, María Eugenia (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Gregori i Font, Josep (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Chen, Qian (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Garcia-Cehic, D. (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Llorens, Meritxell (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
de Ávila, Ana Isabel (Centro de Biología Molecular Severo Ochoa) ;
Beach, Nathan M. (Centro de Biología Molecular Severo Ochoa) ;
Domingo, Esteban (Centro de Biología Molecular Severo Ochoa) ;
Rodríguez-Frías, Francisco (Universitat Autònoma de Barcelona) ;
Buti, Maria (Universitat Autònoma de Barcelona) ;
Esteban, Rafael (Esteban Mur) (Universitat Autònoma de Barcelona) ;
Esteban Mur, Juan Ignacio (Universitat Autònoma de Barcelona) ;
Quer, Josep 1963- (Universitat Autònoma de Barcelona) ;
Perales Viejo, Celia (Centro de Biología Molecular Severo Ochoa)
Despite the high sustained virological response rates achieved with current directly-acting antiviral agents (DAAs) against hepatitis C virus (HCV), around 5-10% of treated patients do not respond to current antiviral therapies, and basal resistance to DAAs is increasingly detected among treatment-naïve infected individuals. [...]
2018 - 10.1186/s12879-018-3356-6
BMC Infectious diseases, Vol. 18 (september 2018)
|
|
7.
|
13 p, 3.0 MB |
Naturally occurring SARS-CoV-2 gene deletions close to the spike S1/S2 cleavage site in the viral quasispecies of COVID19 patients
/
Andrés, Cristina (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Garcia-Cehic, D. (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Gregori i Font, Josep (Roche Diagnostics SL (Sant Cugat del Valles)) ;
Piñana, Maria (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Rodriguez-Frias, Francisco (Universitat Autònoma de Barcelona) ;
Guerrero-Murillo, Mercedes (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Esperalba, Juliana (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Rando, Adriana (Hospital Universitari Vall d'Hebron) ;
Goterris, Lidia (Hospital Universitari Vall d'Hebron) ;
Codina, María Gema (Hospital Universitari Vall d'Hebron) ;
Quer, Susanna (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Martín, Maria Carmen (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Campins Martí, Magda (Hospital Universitari Vall d'Hebron) ;
Ferrer, Ricard (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Almirante Gragera, Benito (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Esteban Mur, Juan Ignacio (Universitat Autònoma de Barcelona) ;
Pumarola Suñé, Tomàs (Universitat Autònoma de Barcelona) ;
Antón, Andrés (Universitat Autònoma de Barcelona) ;
Quer, Josep 1963- (Hospital Universitari Vall d'Hebron. Institut de Recerca)
The SARS-CoV-2 spike (S) protein, the viral mediator for binding and entry into the host cell, has sparked great interest as a target for vaccine development and treatments with neutralizing antibodies. [...]
2020 - 10.1080/22221751.2020.1806735
Emerging microbes & infections, Vol. 9 Núm. 1 (september 2020) , p. 1900-1911
|
|
8.
|
9 p, 1.3 MB |
Whole-genome characterization and resistance-associated substitutions in a new HCV genotype 1 subtype
/
von Massow, Georg (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Garcia-Cehic, D. (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) ;
Gregori i Font, Josep (Roche Diagnostics S.L) ;
Rodriguez-Frias, Francisco (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Macià, María Dolores (Institut d'Investigació Sanitària Illes Balears) ;
Escarda, Ana (Hospital Universitari Son Espases (Palma de Mallorca, Balears)) ;
Esteban Mur, Juan Ignacio (Universitat Autònoma de Barcelona. Departament de Medicina) ;
Quer, Josep 1963- (Universitat Autònoma de Barcelona. Departament de Medicina)
Hepatitis C virus (HCV) is a highly variable infectious agent, classified into 8 genotypes and 86 subtypes. Our laboratory has implemented an in-house developed high-resolution HCV subtyping method based on next-generation sequencing (NGS) for error-free classification of the virus using phylogenetic analysis and analysis of genetic distances in sequences from patient samples compared to reference sequences. [...]
2019 - 10.2147/IDR.S195441
Infection and drug resistance, Vol. 12 (2019) , p. 947-955
|
|
9.
|
4 p, 131.8 KB |
Baseline hepatitis C virus resistance-associated substitutions present at frequencies lower than 15% may be clinically significant
/
Perales Viejo, Celia (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) ;
Chen, Qian (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) ;
Soria, Maria Eugenia (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Gregori i Font, Josep (Roche Diagnostics SL) ;
Garcia-Cehic, D. (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) ;
Nieto-Aponte, Leonardo (Liver Pathology Unit, Department of Biochemistry and Microbiology, HUVH, Barcelona, Spain) ;
Castells, Lluís (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) ;
Imaz, Arkaitz (Hospital Universitari de Bellvitge) ;
Llorens-Revull, Meritxell (Hospital Universitari Vall d'Hebron) ;
Domingo, Esteban (Centro de Biología Molecular Severo Ochoa) ;
Buti, Maria (Universitat Autònoma de Barcelona. Departament de Medicina) ;
Esteban Mur, Juan Ignacio (Universitat Autònoma de Barcelona. Departament de Medicina) ;
Rodríguez Frías, Francisco (Universitat Autònoma de Barcelona. Departament de Medicina) ;
Quer, Josep 1963- (Universitat Autònoma de Barcelona. Departament de Medicina)
Controversy is ongoing about whether a minority mutant present at frequencies below 15% may be clinically relevant and should be considered to guide treatment. Resistance-associated substitution (RAS) studies were performed in patients before and at failure of antiviral treatments using Next-generation hepatitis C virus (HCV) sequencing (NGS). [...]
2018 - 10.2147/IDR.S172226
Infection and drug resistance, Vol. 11 (november 2018) , p. 2207-2210
|
|
10.
|
6 p, 168.2 KB |
Assessment of a Novel Automatic Real-Time PCR Assay on the Cobas 4800 Analyzer as a Screening Platform for Hepatitis C Virus Genotyping in Clinical Practice : Comparison with Massive Sequencing
/
Nieto-Aponte, Leonardo (Hospital Universitari Vall d'Hebron) ;
Quer, Josep 1963- (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Ruiz-Ripa, Alicia (Hospital Universitari Vall d'Hebron) ;
Tabernero, David (Hospital Universitari Vall d'Hebron) ;
González, Carolina (Hospital Universitari Vall d'Hebron) ;
Gregori i Font, Josep (Hospital Universitari Vall d'Hebron) ;
Vila, Marta (Hospital Universitari Vall d'Hebron) ;
Asensio, Miriam (Hospital Universitari Vall d'Hebron) ;
Garcia-Cehic, D. (Hospital Universitari Vall d'Hebron) ;
Ruiz, Gerardo (Hospital Universitari Vall d'Hebron) ;
Chen, Qian (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Ordeig, Laura (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Llorens, Meritxell (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Saez, Montserrat (Hospital Universitari Vall d'Hebron) ;
Esteban Mur, Juan Ignacio (Hospital Universitari Vall d'Hebron) ;
Esteban, Rafael (Esteban Mur) (Hospital Universitari Vall d'Hebron) ;
Buti, Maria (Hospital Universitari Vall d'Hebron) ;
Pumarola, Tomas (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Rodriguez-Frias, Francisco (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Universitat Autònoma de Barcelona
The unequivocal identification of hepatitis C virus (HCV) subtypes 1a/1b and genotypes 2 to 6 is required for optimizing the effectiveness of interferon-free, direct-acting antiviral therapies. We compared the performance of a new real-time HCV genotyping assay used on the Cobas 4800 system (C4800) with that of high-resolution HCV subtyping (HRCS). [...]
2017 - 10.1128/JCM.01960-16
Journal of Clinical Microbiology, Vol. 55 (january 2017) , p. 504-509
|
|