|
1.
|
|
40 p, 1.6 MB |
Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter
/
Cámara, Elena (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Monforte, Sergi (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The methanol-regulated alcohol oxidase promoter (PAOX1) of Pichia pastoris (syn. Komagataella spp. ) is one of the strongest promoters for heterologous gene expression. Although increasing the gene dosage is a common strategy to improve recombinant protein productivities, P. [...]
2019 -  10.1002/bit.26947
Biotechnology and Bioengineering, Vol. 116, Issue 7 (July 2019) , p. 1710-1720
|
|
|
2.
|
|
16 p, 2.4 MB |
Carbohydrate hydrolytic potential and redundancy of an anaerobic digestion microbiome exposed to acidosis, as uncovered by metagenomics
/
Bertucci, Marie (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Calusinska, Magdalena (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Goux, Xavier (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Rouland-Lefèvre, Corinne (Sorbonne Universités. Institut de Recherche Développement. Institut d'Ecologie et des Sciences de l'Environnement) ;
Untereiner, Boris (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Ferrer, Pau (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Gerin, Patrick A. (Université Catholique de Louvain. Laboratory of Bioengineering, Earth) ;
Delfosse, Philippe (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department)
Increased hydrolysis of easily digestible biomass may lead to acidosis of anaerobic reactors and decreased methane production. Previously, it was shown that the structure of microbial communities changed during acidosis; however, once the conditions are back to optimal, biogas (initially CO2) production quickly restarts. [...]
2019 - 10.1128/AEM.00895-19
Applied and Environmental Microbiology, Vol. 85, Issue 15 (August 2019) , p. e00895-19
|
|
|
3.
|
|
|
4.
|
|
11 p, 1.7 MB |
Combining metabolic engineering and multiplexed screening methods for 3-Hydroxypropionic acid production in Pichia pastoris
/
Fina Romero, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Heux, Stephanie (Université de Toulouse. Toulouse Biotechnology Institute) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Production of 3-hydroxypropionic acid (3-HP) in Pichia pastoris (syn. Komagataella phaffii) via the malonyl-CoA pathway has been recently demonstrated using glycerol as a carbon source, but the reported metrics were not commercially relevant. [...]
2022 - 10.3389/fbioe.2022.942304
Frontiers in Bioengineering and Biotechnology, Vol. 10 (July 2022) , art. 942304
2 documents
|
|
|
5.
|
|
11 p, 1.6 MB |
Process intensification at the expression system level for the production of 1-phosphate aldolase in antibiotic-free E. coli fed-batch cultures
/
Pasini, Martina (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Fernández-Castané, Alfred (Aston University. Aston Institute of Materials Research) ;
Caminal i Saperas, Glòria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Overton, Tim W. (University of Birmingham. College of Engineering and Physical Sciences) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
To successfully design expression systems for industrial biotechnology and biopharmaceutical applications; plasmid stability, efficient synthesis of the desired product and the use of selection markers acceptable to regulatory bodies are of utmost importance. [...]
2022 - 10.1093/jimb/kuac018
Journal of Industrial Microbiology and Biotechnology, Vol. 49, Issue 4 (July 2022) , art. kuac018
|
|
|
6.
|
|
17 p, 14.9 MB |
Expression of Chimeric HPV-HIV Protein L1P18 in Pichia pastoris; Purification and Characterization of the Virus-like Particles
/
Eto, Yoshiki (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Saubi, Narcis (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Joseph-Munné, Joan (Hospital Universitari Vall d'Hebron)
Currently, three human papillomavirus (HPV) vaccines are already licensed and all of them are based on virus-like particles (VLPs) of HPV L1 capsid protein but not worldwide accessible. While about 38. [...]
2021 - 10.3390/pharmaceutics13111967
Pharmaceutics, Vol. 13, Issue 11 (November 2021) , art. 1967
|
|
|
7.
|
|
30 p, 2.4 MB |
Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris
/
De, Sonakshi (BOKU University of Natural Resources and Life Sciences Vienna. Department of Biotechnology) ;
Mattanovich, Diethard (BOKU University of Natural Resources and Life Sciences Vienna. Department of Biotechnology) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gasser, Brigitte (BOKU University of Natural Resources and Life Sciences Vienna. Christian Doppler Laboratory for growth-decoupled protein production in yeast)
Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris) has been developed into the most popular yeast cell factory for the production of heterologous proteins. [...]
2021 - 10.1042/EBC20200138
Essays in Biochemistry, Vol. 65, Issue 2 (July 2021) , p. 293-307
|
|
|
8.
|
|
12 p, 239.6 KB |
Benchmarking recombinant Pichia pastoris for 3-hydroxypropionic acid production from glycerol
/
Fina Romero, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Breda, Gabriela (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Pérez-Trujillo, Míriam (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear) ;
Guimaraes Freire, Denise Maria (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Volcan Almeida, Rodrigo (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The use of the methylotrophic yeast Pichia pastoris (Komagataella phaffi) to produce heterologous proteins has been largely reported. However, investigations addressing the potential of this yeast to produce bulk chemicals are still scarce. [...]
2021 - 10.1111/1751-7915.13833
Microbial Biotechnology, Vol. 14, Issue 4 (July 2021) , p. 1671-1682
|
|
|
9.
|
|
2 p, 637.4 KB |
Enginyeria metabòlica de llevats per a la producció de bioplàstics
/
Fina Romero, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
La biotecnologia és clau per poder implementar un model d'economia circular i assolir un desenvolupament sostenible i independent del petroli. Un projecte del Grup de Recerca ENG4BIO ha construït, mitjançant enginyeria metabòlica, un llevat capaç de convertir el glicerol cru, un residu de la producció de biodièsel, en àcid 3-hidroxipropiònic, un precursor per a la síntesi de bioplàstics.
La biotecnología es clave para poder implementar un modelo de economía circular y conseguir un desarrollo sostenible e independiente del petróleo. Un proyecto del Grupo de Investigación ENG4BIO ha construido, mediante ingeniería metabólica, una levadura capaz de convertir el glicerol crudo, un residuo de la producción de biodiesel, en ácido 3-hidroxipropiónico, un precursor para la síntesis de bioplásticos.
2022
UAB divulga, Maig 2022
2 documents
|
|
|
10.
|
|
12 p, 2.0 MB |
Integrative omics analysis of the termite gut system adaptation to Miscanthus diet identifies lignocellulose degradation enzymes
/
Calusinska, Magdalena (Luxembourg Institute of Science and Technology. BioSystems and Bioprocessing Engineering) ;
Marynowska, Martyna (Université Libre de Bruxelles. Evolutionary Biology and Ecology) ;
Bertucci, Marie (Université Catholique de Louvain. Laboratory of Bioengineering, Earth and Life Institute) ;
Untereiner, Boris (Luxembourg Institute of Science and Technology. BioSystems and Bioprocessing Engineering) ;
Klimek, Dominika (Luxembourg Institute of Science and Technology. BioSystems and Bioprocessing Engineering) ;
Goux, Xavier (Luxembourg Institute of Science and Technology. BioSystems and Bioprocessing Engineering) ;
Sillam-Dussès, David (University Paris 13-Sorbonne Paris Cité. Laboratory of Experimental and Comparative Ethology) ;
Gawron, Piotr (University of Luxembourg. Luxembourg Centre for Systems Biomedicine) ;
Halder, Rashi (University of Luxembourg. Luxembourg Centre for Systems Biomedicine) ;
Wilmes, Paul (University of Luxembourg. Luxembourg Centre for Systems Biomedicine) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gerin, Patrick (Université Catholique de Louvain. Laboratory of Bioengineering, Earth and Life Institute) ;
Roisin, Yves (Université Libre de Bruxelles. Evolutionary Biology and Ecology) ;
Delfosse, Philippe (University of Luxembourg)
Miscanthus sp. biomass could satisfy future biorefinery value chains. However, its use is largely untapped due to high recalcitrance. The termite and its gut microbiome are considered the most efficient lignocellulose degrading system in nature. [...]
2020 - 10.1038/s42003-020-1004-3
Communications Biology, Vol. 3 (June 2020) , art. 275
|
|
visitant ::
identificació
alerta personal via correu electrònic o subscribiu-vos al 
canal RSS.
