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Articles, 2 records found
Articles 2 records found  
1.
11 p, 2.5 MB A bead-assisted flow cytometry method for the semi-quantitative analysis of Extracellular Vesicles / Suárez, Henar (Instituto de Investigación Sanitaria La Princesa) ; Gámez-Valero, Ana (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Reyes, Raquel (Centro de Biología Molecular Severo Ochoa) ; López-Martín, Soraya (Instituto de Investigación Sanitaria La Princesa) ; Rodríguez, María Josefa (Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas) ; Carrascosa, José L. (Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas) ; Cabañas, Carlos (Centro de Biología Molecular Severo Ochoa) ; Borràs, Francesc E. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Yáñez-Mó, María (Instituto de Investigación Sanitaria La Princesa) ; Universitat Autònoma de Barcelona
Most experimental approaches commonly employed for the characterization and quantitation of EVs are time consuming, require of specialized instrumentation and often are rather inaccurate. To circumvent the caveats imposed by EV small size, we used general and specific membrane markers in bead assisted flow cytometry, to provide a semi-quantitative measure of EV content in a given sample. [...]
2017 - 10.1038/s41598-017-11249-2
Scientific reports, Vol. 7 (september 2017) , p. 1-11  
2.
9 p, 1.4 MB Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles' characteristics compared to precipitating agents / Gámez Valero, Ana (Institut Germans Trias i Pujol) ; Monguió Tortajada, Marta (Institut Germans Trias i Pujol) ; Carreras Planella, Laura (Institut Germans Trias i Pujol) ; Franquesa, Marcel·la (Institut Germans Trias i Pujol) ; Beyer, Katrin (Institut Germans Trias i Pujol) ; Borràs, Francesc E. (Institut Germans Trias i Pujol)
Extracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation has been the most widely used methodology but rapid methods, including Size Exclusion Chromatography (SEC) and/or precipitating agents such as Polyethylene glycol (PEG) or PRotein Organic Solvent PRecipitation (PROSPR) have emerged. [...]
2016 - 10.1038/srep33641
Scientific reports, Vol. 6 (September 2016)  

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1 Gámez-Valero, Ana
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