1.
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16 p, 2.4 MB |
Gag Virus-like Particles Functionalized with SARS-CoV-2 Variants: Generation, Characterization and Recognition by COVID-19 Convalescent Patients' Sera
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Boix-Besora, Arnau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The robustness, safety, versatility, and high immunogenicity of virus-like particles (VLPs) make them a promising approach for the generation of vaccines against a broad range of pathogens. VLPs are recombinant macromolecular structures that closely mimic the native conformation of viruses without carrying viral genetic material. [...]
2023 - 10.3390/vaccines11111641
Vaccines (Basel), Vol. 11, Issue 11 (October 2023) , art. 1641
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2.
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13 p, 1.2 MB |
Downstream process design for Gag HIV-1 based virus-like particles
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Lorenzo, Elianet (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Miranda, Laia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Virus-like particles-based vaccines have been gaining interest in recent years. The manufacturing of these particles includes their production by cell culture followed by their purification to meet the requirements of its final use. [...]
2023 - 10.1002/bit.28419
Biotechnology and Bioengineering, Vol. 120, Issue 9 (September 2023) , p. 2672-2684
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3.
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13 p, 2.7 MB |
An engineered HIV-1 Gag-based VLP displaying high antigen density induces strong antibody-dependent functional immune responses
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Tarrés-Freixas, Ferran (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Aguilar-Gurrieri, Carmen (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Rodríguez de la Concepción, María Luisa (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Urrea, Víctor (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Trinité, Benjamin (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Ortiz, Raquel (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Pradenas, Edwards (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Blanco, Pau (Institut Germans Trias i Pujol) ;
Marfil, Sílvia (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Molinos-Albert, Luis Manuel ;
Barajas Vélez, Ana (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Pons-Grífols, Anna (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Avila-Nieto, Carlos (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Varela, Ismael (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona) ;
Gutiérrez-Granados, Sònia (Universitat Autònoma de Barcelona) ;
Segura, María Mercedes (Universitat Autònoma de Barcelona) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona) ;
Clotet Sala, Bonaventura (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Carrillo, Jorge (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa) ;
Blanco, Julià (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Antigen display on the surface of Virus-Like Particles (VLPs) improves immunogenicity compared to soluble proteins. We hypothesised that immune responses can be further improved by increasing the antigen density on the surface of VLPs. [...]
2023 - 10.1038/s41541-023-00648-4
NPJ Vaccines, Vol. 8 (april 2023)
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4.
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10 p, 3.7 MB |
Bioprocess characterization of virus-like particle production with the insect cell baculovirus expression system at nanoparticle level
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Puente-Massaguer, Eduard (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
González-Domínguez, Irene (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Strobl, Florian (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria)) ;
Grabherr, Reingard (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria)) ;
Striedner, Gerald (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria)) ;
Lecina i Veciana, Martí (Universitat Ramon Llull. Institut Químic de Sarrià-IQS) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background. Virus-like particles (VLPs) are a multivalent platform showing great promise for the development of vaccines, gene therapy, diagnostic, and drug delivery approaches. Particularly, HIV-1 Gag VLPs provide a robust and flexible scaffold for the presentation of a variety of antigens. [...]
2022 - 10.1002/jctb.7105
Journal of chemical technology and biotechnology, Vol. 97, Issue 9 (September 2022) , p. 2456-2465
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5.
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20 p, 10.2 MB |
Enhancing control systems of higher plant culture chambers via multilevel structural mechanistic modelling
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Ciurans, Carles (Universitat Autònoma de Barcelona) ;
Guerrero, Josep M. (Aalborg University. Centre for Research on Microgrids) ;
Martínez-Mongue, Ivan (Novo Nordisk Foundation Center for Sustainability) ;
Dussap, Claude G. (Université Clermont Auvergne. Institut Pascal) ;
Marin de Mas, Igor (Novo Nordisk Foundation Center for Sustainability) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona)
Modelling higher plant growth is of strategic interest for modern agriculture as well as for the development of bioregenerative life support systems for space applications, where crop growth is expected to play an essential role. [...]
2022 - 10.3389/fpls.2022.970410
Frontiers in plant science, Vol. 13 (October 2022) , art. 970410
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6.
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14 p, 2.7 MB |
The cell density effect in animal cell-based bioprocessing : Questions, insights and perspectives
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Lavado-García, Jesús (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Pérez-Rubio, Pol (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
One of the main challenges in the development of bioprocesses based on cell transient expression is the commonly reported reduction of cell specific productivity at increasing cell densities. This is generally known as the cell density effect (CDE). [...]
2022 - 10.1016/j.biotechadv.2022.108017
Biotechnology advances, Vol. 60 (November 2022) , art. 108017
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7.
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15 p, 2.8 MB |
Differential N- and O-glycosylation signatures of HIV-1 Gag virus-like particles and coproduced extracellular vesicles
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Lavado-García, Jesús (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Zhang, Tao (Leiden University Medical Center. Center for Proteomics and Metabolomics) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Wuhrer, Manfred (Leiden University Medical Center. Center for Proteomics and Metabolomics)
Human immunodeficiency virus 1 (HIV-1) virus-like particles (VLPs) are nanostructures derived from the self-assembly and cell budding of Gag polyprotein. Mimicking the native structure of the virus and being noninfectious, they represent promising candidates for the development of new vaccines as they elicit a strong immune response. [...]
2022 - 10.1002/bit.28051
Biotechnology and Bioengineering, Vol. 119, Issue 5 (May 2022) , p. 1207-1221
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8.
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16 p, 2.6 MB |
Transduction of HEK293 Cells with BacMam Baculovirus Is an Efficient System for the Production of HIV-1 Virus-like Particles
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Puente-Massaguer, Eduard (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cajamarca-Berrezueta, Byron (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Volart, Aleix (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
González-Domínguez, Irene (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gag virus-like particles (VLPs) are promising vaccine candidates against infectious diseases. VLPs are generally produced using the insect cell/baculovirus expression vector system (BEVS), or in mammalian cells by plasmid DNA transient gene expression (TGE). [...]
2022 - 10.3390/v14030636
Viruses, Vol. 14, Num. 3 (March 2022) , art. 636
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9.
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16 p, 2.2 MB |
Metaproteomics, Heterotrophic Growth, and Distribution of Nitrosomonas europaea and Nitrobacter winogradskyi after Long-Term Operation of an Autotrophic Nitrifying Biofilm Reactor
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Mastroleo, Felice (Belgian Nuclear Research Centre) ;
Arnau Jiménez, Carolina (Universitat Autònoma de Barcelona) ;
Verbeelen, Tom (Ghent University) ;
Mysara, Mohamed (Belgian Nuclear Research Centre) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona) ;
Leys, Natalie (Belgian Nuclear Research Centre) ;
Van Houdt, Rob (Belgian Nuclear Research Centre)
Bioregenerative life support systems (BLSS) are currently in development to tackle low recovery efficiencies, high energy demands, as well as food, water, and oxygen production challenges through the regeneration of nutrients from waste streams. [...]
2022 - 10.3390/applmicrobiol2010020
Applied Microbiology, Vol. 2, Num. 1 (March 2022) , p. 272-287
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10.
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19 p, 4.2 MB |
A four-step purification process for gag vlps : from culture supernatant to high-purity lyophilized particles
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González-Domínguez, Irene (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Lorenzo, Elianet (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Bernier, Alice (McGill University. Department of Bioengineering) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Kamen, Amine (McGill University. Department of Bioengineering)
Gag-based virus-like particles (VLPs) have high potential as scaffolds for the development of chimeric vaccines and delivery strategies. The production of purified preparations that can be preserved independently from cold chains is highly desirable to facilitate distribution and access worldwide. [...]
2021 - 10.3390/vaccines9101154
Vaccines (Basel), Vol. 9, Issue 10 (October 2021) , art. 1154
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