1.
|
10 p, 3.7 MB |
Bioprocess characterization of virus-like particle production with the insect cell baculovirus expression system at nanoparticle level
/
Puente-Massaguer, Eduard (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
González-Domínguez, Irene (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Strobl, Florian (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria)) ;
Grabherr, Reingard (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria)) ;
Striedner, Gerald (University of Natural Resources and Life Sciences. Department of Biotechnology (Austria)) ;
Lecina, Martí (Universitat Ramon Llull. Institut Químic de Sarrià-IQS) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background. Virus-like particles (VLPs) are a multivalent platform showing great promise for the development of vaccines, gene therapy, diagnostic, and drug delivery approaches. Particularly, HIV-1 Gag VLPs provide a robust and flexible scaffold for the presentation of a variety of antigens. [...]
2022 - 10.1002/jctb.7105
Journal of chemical technology and biotechnology, Vol. 97, Issue 9 (September 2022) , p. 2456-2465
|
|
2.
|
14 p, 2.7 MB |
The cell density effect in animal cell-based bioprocessing : Questions, insights and perspectives
/
Lavado-García, Jesús (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Pérez-Rubio, Pol (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
One of the main challenges in the development of bioprocesses based on cell transient expression is the commonly reported reduction of cell specific productivity at increasing cell densities. This is generally known as the cell density effect (CDE). [...]
2022 - 10.1016/j.biotechadv.2022.108017
Biotechnology advances, Vol. 60 (November 2022) , art. 108017
|
|
3.
|
15 p, 2.8 MB |
Differential N- and O-glycosylation signatures of HIV-1 Gag virus-like particles and coproduced extracellular vesicles
/
Lavado-García, Jesús (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Zhang, Tao (Leiden University Medical Center. Center for Proteomics and Metabolomics) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Wuhrer, Manfred (Leiden University Medical Center. Center for Proteomics and Metabolomics)
Human immunodeficiency virus 1 (HIV-1) virus-like particles (VLPs) are nanostructures derived from the self-assembly and cell budding of Gag polyprotein. Mimicking the native structure of the virus and being noninfectious, they represent promising candidates for the development of new vaccines as they elicit a strong immune response. [...]
2022 - 10.1002/bit.28051
Biotechnology and Bioengineering, Vol. 119, Issue 5 (May 2022) , p. 1207-1221
|
|
4.
|
10 p, 5.7 MB |
Stability enhancement of clinical grade multipotent mesenchymal stromal cell-based products
/
Mirabel, Clémentine (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Puente-Massaguer, Eduard (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
del Mazo-Barbara, Anna (Banc de Sang i Teixits. Servei de Teràpia Cel·lular) ;
Reyes, Blanca (Banc de Sang i Teixits. Servei de Teràpia Cel·lular) ;
Morton, Philip (Albumedix Ltd (Nottingham)) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Vives Armengol, Joaquim (Universitat Autònoma de Barcelona. Departament de Medicina)
Successful delivery of cell-based therapeutics into patients is compromised by their short shelf-life upon release from production facilities due to the living nature of the active component that rapidly loses viability, and therefore its properties. [...]
2018 - 10.1186/s12967-018-1659-4
Journal of translational medicine, Vol. 16 (october 2018)
|
|
5.
|
15 p, 3.9 MB |
Hierarchically controlled ecological life support systems
/
Ciurans, Carles (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Bazmohammadi, Najmeh (Aalborg University. Centre for Research on Microgrids) ;
Poughon, Laurent (Campus Universitaire des Cézeaux. Institut Pascal) ;
Vasquez, Juan (Aalborg University. Centre for Research on Microgrids) ;
Dussap, Claude G. (Campus Universitaire des Cézeaux. Institut Pascal) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Guerrero, Josep Maria (Aalborg University. Centre for Research on Microgrids)
Life support systems (LSSs) are autonomous systems integrating various generation, recycling, and consumption subsystems with storage capability to maintain the balance of key system compounds. A novel hierarchical control is proposed in this paper to operate an exemplary controlled ecological LSS. [...]
2022 - 10.1016/j.compchemeng.2021.107625
Computers and chemical engineering, Vol. 157 (January 2022) , art. 107625
|
|
6.
|
17 p, 4.6 MB |
Chimeric VLPs Based on HIV-1 Gag and a Fusion Rabies Glycoprotein Induce Specific Antibodies against Rabies and Foot-and-Mouth Disease Virus
/
Fontana, Diego (Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas) ;
Cevera, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Kratje, Ricardo (Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas) ;
Prieto, Claudio (Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Garay, Ernesto (Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas)
Foot and mouth disease is a livestock acute disease, causing economic losses in affected areas. Currently, control of this disease is performed by mandatory vaccination campaigns using inactivated viral vaccines. [...]
2021 - 10.3390/vaccines9030251
Vaccines, Vol. 9 Núm. 3 (2021) , p. 251
|
|
7.
|
16 p, 3.7 MB |
PEI-mediated transient transfection of High Five cells at bioreactor scale for HIV-1 VLP production
/
Puente-Massaguer, Eduard (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Strobl, Florian (University of Natural Resources and Life Sciences. Department of Biotechnology) ;
Grabherr, Reingard (University of Natural Resources and Life Sciences. Department of Biotechnology) ;
Striedner, Gerald (University of Natural Resources and Life Sciences. Department of Biotechnology) ;
Lecina i Veciana, Martí (Universitat Ramon Llull. Institut Químic de Sarrià-IQS) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
High Five cells are an excellent host for the production of virus-like particles (VLPs) with the baculovirus expression vector system (BEVS). However, the concurrent production of high titers of baculovirus hinder the purification of these nanoparticles due to similarities in their physicochemical properties. [...]
2020 - 10.3390/nano10081580
Nanomaterials, Vol. 10, Núm. 8 (August 2020) , art. 1580
|
|
8.
|
|
9.
|
24 p, 8.2 MB |
Quality Assessment of Virus-Like Particles at Single Particle Level : A Comparative Study
/
González-Domínguez, Irene (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Puente-Massaguer, Eduard (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Virus-like particles (VLPs) have emerged as a powerful scaffold for antigen presentation and delivery strategies. Compared to single protein-based therapeutics, quality assessment requires a higher degree of refinement due to the structure of VLPs and their similar properties to extracellular vesicles (EVs). [...]
2020 - 10.3390/v12020223
Viruses, Vol. 12, Issue 2 (February 2020) , art. 223
|
|
10.
|
11 p, 1.7 MB |
At-line multi-angle light scattering detector for faster process development in enveloped virus-like particle purification
/
Pereira Aguilar, P. (University of Natural Resources and Life Sciences. Department of Biotechnology) ;
González-Domínguez, Irene (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Schneider, Tobias Amadeus (Austrian Centre of Industrial Biotechnology (ACIB)) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Jungbauer, Alois (Austrian Centre of Industrial Biotechnology (ACIB))
At-line static light scattering and fluorescence monitoring allows direct in-process tracking of fluorescent virus-like particles. We have demonstrated this by coupling at-line multi-angle light scattering and fluorescence detectors to the downstream processing of enveloped virus-like particles. [...]
2019 - 10.1002/jssc.201900441
Journal of Separation Science, Vol. 42, Num. 16 (2019) , p. 2640-2649
|
|