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21 p, 4.7 MB Extending Immunological Profiling in the Gilthead Sea Bream, Sparus aurata, by Enriched cDNA Library Analysis, Microarray Design and Initial Studies upon the Inflammatory Response to PAMPs / Boltaña Harms, Sebastian (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Castellana, Barbara (The Child & Family Research Institute (Vancouver, Canadà)) ; Goetz, Giles (Northwest Fisheries Science Centre (Seattle, Estats Units d'Amèrica)) ; Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia) ; Teles, Mariana (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia) ; Mulero, Víctor (Universidad de Murcia. Department de Biología Celular e Histología) ; Novoa, Beatriz (Instituto de Investigaciones Marinas (Vigo, Espanya)) ; Figueras, Antonio (Instituto de Investigaciones Marinas (Vigo, Espanya)) ; Goetz, Frederick W. (Northwest Fisheries Science Centre (Seattle, Estats Units d'Amèrica)) ; Gallardo-Escarate, Cristian (Universidad de Concepción (Concepción, Xile)) ; Planas, Josep V. (Universitat de Barcelona. Departament de Fisiologia i Immunologia) ; Mackenzie, Simon A. (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
This study describes the development and validation of an enriched oligonucleotide-microarray platform for Sparus aurata (SAQ) to provide a platform for transcriptomic studies in this species. A transcriptome database was constructed by assembly of gilthead sea bream sequences derived from public repositories of mRNA together with reads from a large collection of expressed sequence tags (EST) from two extensive targeted cDNA libraries characterizing mRNA transcripts regulated by both bacterial and viral challenge. [...]
2017 - 10.3390/ijms18020317
International Journal of Molecular Sciences, Vol. 18 Núm. 2 (2017) , p. 1-21  

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