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12 p, 2.9 MB |
The biological potential hidden in inclusion bodies
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Gifre-Renom, Laia (Institut de Recerca i Tecnologia Agroalimentàries) ;
Seras-Franzoso, Joaquín (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Rafael, Diana (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Andrade, Fernanda (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Martinez-Trucharte, Francesc (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Ugarte-Berzal, Estefanía (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation) ;
Martens, Erik (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation) ;
Boon, Lise (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Opdenakker, Ghislain (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation) ;
Schwartz, Simon (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries) ;
García Fruitós, Elena (Institut de Recerca i Tecnologia Agroalimentàries)
Inclusion bodies (IBs) are protein nanoclusters obtained during recombinant protein production processes, and several studies have demonstrated their potential as biomaterials for therapeutic protein delivery. [...]
2020 -  10.3390/pharmaceutics12020157
Pharmaceutics, Vol. 12, issue 2 (2020) , art. 157
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13 p, 1.9 MB |
Recombinant protein-based nanoparticles : elucidating their inflammatory effects in vivo and their potential as a new therapeutic format
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Gifre-Renom, Laia (Institut de Recerca i Tecnologia Agroalimentàries) ;
Ugarte-Berzal, Estefanía (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation) ;
Martens, Erik (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation) ;
Boon, Lise (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation) ;
Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Martínez-Núñez, Esther (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Luque, Teresa (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Roca-Pinilla, Ramon (Institut de Recerca i Tecnologia Agroalimentàries) ;
Conchillo-Solé, Óscar (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Opdenakker, Ghislain (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation) ;
García Fruitós, Elena (Institut de Recerca i Tecnologia Agroalimentàries) ;
Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries)
Bacterial inclusion bodies (IBs) are protein-based nanoparticles of a few hundred nanometers formed during recombinant protein production processes in different bacterial hosts. IBs contain active protein in a mechanically stable nanostructured format that has been broadly characterized, showing promising potential in different fields such as tissue engineering, protein replacement therapies, cancer, and biotechnology. [...]
2020 - 10.3390/pharmaceutics12050450
Pharmaceutics, Vol. 12, issue 5 (2020) , art. 450
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10 p, 1.5 MB |
A new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates
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Gifre-Renom, Laia (Institut de Recerca i Tecnologia Agroalimentàries) ;
Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Fàbregas, F. (Institut de Recerca i Tecnologia Agroalimentàries) ;
Roca-Pinilla, R. (Institut de Recerca i Tecnologia Agroalimentàries) ;
Seras-Franzoso, Joaquín (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Bach, Àlex (Institució Catalana de Recerca i Estudis Avançats) ;
Devant, M. (Institut de Recerca i Tecnologia Agroalimentàries) ;
Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries) ;
García Fruitós, Elena (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
The production of pure and soluble proteins is a complex, protein-dependent and time-consuming process, in particular for those prone-to-aggregate and/or difficult-to-purify. Although Escherichia coli is widely used for protein production, recombinant products must be co-purified through costly processes to remove lipopolysaccharide (LPS) and minimize adverse effects in the target organism. [...]
2018 - 10.1038/s41598-018-32213-8
Scientific reports, Vol. 8 (2018) , art. 13917
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12 p, 5.9 MB |
CXCR4+-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis
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Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Céspedes, Maria Virtudes (Institut d'Investigació Biomèdica Sant Pau) ;
Unzueta, Ugutz (Institut d'Investigació Biomèdica Sant Pau) ;
Saccardo, P. (CIBER de Bioingeniería. Biomateriales y Nanomedicina (CIBER-BBN)) ;
Roldán, M. (Servei de Microscòpia. Universitat Autònoma de Barcelona) ;
Sánchez Chardi, Alejandro (Universitat Autònoma de Barcelona. Servei de Microscòpia) ;
Cubarsi, R. (Departament de Matemàtica Aplicada IV. Universitat Politècnica de Catalunya) ;
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Mangues, Ramon (Institut d'Investigació Biomèdica Sant Pau) ;
García Fruitós, Elena (Institut de Recerca i Tecnologia Agroalimentàries) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Universitat Autònoma de Barcelona
Aim: Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-assembling protein materials. [...]
2016 - 10.2217/nnm-2016-0200
Nanomedicine, Vol. 11, issue 18 (2016) , p. 2387-2398
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12 p, 3.2 MB |
Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli
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Rueda, Fabian (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Céspedes, Maria Virtudes (Institut d'Investigació Biomèdica Sant Pau) ;
Sánchez Chardi, Alejandro (Universitat Autònoma de Barcelona. Servei de Microscòpia) ;
Seras-Franzoso, Joaquín (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Pesarrodona Roches, Mireia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Rinas, Ursula (Helmholtz Centre for Infection Research) ;
Unzueta, Ugutz (Institut d'Investigació Biomèdica Sant Pau) ;
Mamat, Uwe (Airway Research Center North) ;
Mangues, Ramon (Institut d'Investigació Biomèdica Sant Pau) ;
García Fruitós, Elena (Institut de Recerca i Tecnologia Agroalimentàries) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Universitat Autònoma de Barcelona
Background: production of recombinant drugs in process-friendly endotoxin-free bacterial factories targets to a lessened complexity of the purification process combined with minimized biological hazards during product application. [...]
2016 - 10.1186/s12934-016-0457-z
Microbial cell factories, Vol. 15 (2016) , art. 59
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12 p, 1.7 MB |
Functional inclusion bodies produced in the yeast Pichia pastoris
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Rueda, Fabián (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Gasser, Brigitte (Austrian Centre of Industrial Biotechnology) ;
Sánchez Chardi, Alejandro (Universitat Autònoma de Barcelona. Servei de Microscòpia) ;
Roldán, Mónica (Universitat Autònoma de Barcelona. Servei de Microscòpia) ;
Villegas Hernández, Sandra (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular) ;
Puxbaum, Verena (Austrian Centre of Industrial Biotechnology (ACIB)) ;
Ferrer Miralles, Neus (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Unzueta, Ugutz (Institut d'Investigació Biomèdica Sant Pau) ;
Vázquez Gómez, Esther (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
García Fruitós, Elena (Institut de Recerca i Tecnologia Agroalimentàries) ;
Mattanovich, Diethard (Austrian Centre of Industrial Biotechnology) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Universitat Autònoma de Barcelona
Background: Bacterial inclusion bodies (IBs) are non-toxic protein aggregates commonly produced in recombinant bacteria. They are formed by a mixture of highly stable amyloid-like fibrils and releasable protein species with a significant extent of secondary structure, and are often functional. [...]
2016 - 10.1186/s12934-016-0565-9
Microbial cell factories, Vol. 15 (2016) , art. 166
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12 p, 1.8 MB |
Lactic acid bacteria : reviewing the potential of a promising delivery live vector for biomedical purposes
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Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Seras-Franzoso, Joaquín (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
García Fruitós, Elena (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Lactic acid bacteria (LAB) have a long history of safe exploitation by humans, being used for centuries in food production and preservation and as probiotic agents to promote human health. Interestingly, some species of these Gram-positive bacteria, which are generally recognized as safe organisms by the US Food and Drug Administration (FDA), are able to survive through the gastrointestinal tract (GIT), being capable to reach and colonize the intestine, where they play an important role. [...]
2015 - 10.1186/s12934-015-0313-6
Microbial cell factories, Vol. 14 (2015) , art. 137
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27 p, 1.5 MB |
On the Suitability of Raman Spectroscopy to Monitor the Degree of Graphene Functionalization by Diazonium Salts
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Sampathkumar, Krishna (Brno University of Technology) ;
Díez Cabanes, Valentín (University of Mons) ;
Kovaricek, Petr (J.Heyrovsky Institute of Physical Chemistry) ;
Del Corro García, Elena (Institut Català de Nanociència i Nanotecnologia) ;
Bouša, Milan (J.Heyrovsky Institute of Physical Chemistry) ;
Hošek, Jan (Czech Technical University in Prague) ;
Kalbáč, Martin (J.Heyrovsky Institute of Physical Chemistry) ;
Frank, Otakar (J.Heyrovsky Institute of Physical Chemistry)
Raman spectroscopy is undoubtedly the most frequently used technique for universal characterization of graphene and related materials. Quantification of parameters like disorder or strain is possible through analysis of particular Raman bands. [...]
2019 - 10.1021/acs.jpcc.9b06516
Journal of physical chemistry, Vol. 123, Issue 36 (September 2019) , p. 22397-22402
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