Results overview: Found 3 records in 0.01 seconds.
Articles, 3 records found
Articles 3 records found  
1.
5 p, 230.8 KB Learning about protein solubility from bacterial inclusion bodies / Martínez Alonso, Mónica (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; González Montalbán, Nuria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; García Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Villaverde Corrales, Antonio (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia
The progressive solving of the conformation of aggregated proteins and the conceptual understanding of the biology of inclusion bodies in recombinant bacteria is providing exciting insights on protein folding and quality. [...]
2009 - 10.1186/1475-2859-8-4
Microbial Cell Factories, Vol. 8, Issue 4 (January 2009)  
2.
6 p, 362.1 KB Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins / Garcia Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; González Montalbán, Nuria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Morell Fernández, Montse (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Vera Barrón, Andrea (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ferraz Colomina, Rosa (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Arís i Giralt, Anna (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ventura i Zamora, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; González Montalbán, Núria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ferraz Colomina, Rosa Maria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ventura Zamora, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Background: Many enzymes of industrial interest are not in the market since they are bio-produced as bacterial inclusion bodies, believed to be biologically inert aggregates of insoluble protein. Results: By using two structurally and functionally different model enzymes and two fluorescent proteins we show that physiological aggregation in bacteria might only result in a moderate loss of biological activity and that inclusion bodies can be used in reaction mixtures for efficient catalysis. [...]
2005 - 10.1186/1475-2859-4-27
Microbial cell factories, Vol. 4, N. 27 (September 2005) , p. 1-6  
3.
9 p, 405.6 KB The chaperone DnaK controls the fractioning of functional protein between soluble and insoluble cell fractions in inclusion body-forming cells / González Montalbán, Núria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Garcia Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ventura i Zamora, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ; Arís i Giralt, Anna (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ventura Zamora, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Background: The molecular mechanics of inclusion body formation is still far from being completely understood, specially regarding the occurrence of properly folded, protein species that exhibit natural biological activities. [...]
2006 - 10.1186/1475-2859-5-26
Microbial cell factories, Vol. 5, N. 26 (August 2006) , p. 1-9  

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