Results overview: Found 9 records in 0.02 seconds.
Articles, 9 records found
Articles 9 records found  
1.
16 p, 1.8 MB Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris / Cámara, Elena (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Landes, Nils (University of Natural Resources and Life Sciences Vienna) ; Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Gasser, Brigitte (Universität für Bodenkultur Wien) ; Mattanovich, Diethard (Universität für Bodenkultur Wien) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The methanol-regulated alcohol oxidase promoter (PAOX1) of Pichia pastoris is one of the strongest promoters for heterologous gene expression in this methylotrophic yeast. Although increasing gene dosage is one of the most common strategies to increase recombinant protein productivities, the increase of gene dosage of Rhizopus oryzae lipase (ROL) in P. [...]
2017 - 10.1038/srep44302
Scientific reports, Vol. 7 (2017) , art. 44302  
2.
14 p, 1.6 MB Golden Pi CS : a golden gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris / Prielhofer, Roland (Austrian Centre of Industrial Biotechnology) ; Barrero, Juan J. (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Steuer, Stefanie (Universität für Bodenkultur Wien) ; Gassler, Thomas (Universität für Bodenkultur Wien) ; Zahrl, Richard (Universität für Bodenkultur Wien) ; Baumann, Kristin (Universität für Bodenkultur Wien) ; Sauer, Michael (Universität für Bodenkultur Wien) ; Mattanovich, Diethard (Universität für Bodenkultur Wien) ; Gasser, Brigitte (Universität für Bodenkultur Wien) ; a Marx, Hans (Universität für Bodenkultur Wien)
State-of-the-art strain engineering techniques for the host Pichia pastoris (syn. Komagataella spp. ) include overexpression of homologous and heterologous genes, and deletion of host genes. For metabolic and cell engineering purposes the simultaneous overexpression of more than one gene would often be required. [...]
2017 - 10.1186/s12918-017-0492-3
BMC systems biology, Vol. 11 (Dec. 2017) , art. 123  
3.
6 p, 267.7 KB The scientific impact of microbial cell factories / De Felice, Maurilio (Università degli Studi di Napoli Federico II. Department of Structural and Functional Biology) ; Mattanovich, Diethard (Universität für Bodenkultur Wien. Department of Biotechnology) ; Papagianni, Maria (Aristotle University of Thessaloniki. School of Veterinary Medicine) ; Wegrzyn, Grzegorz (Uniwersytet Gdański. Department of Molecular Biology) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Institut de Biotecnologia i Biomedicina "Vicent Villar Palasí"
2008 - 10.1186/1475-2859-7-33
Microbial cell factories, Vol. 7, Issue 33 (Desember 2008)  
4.
18 p, 567.4 KB Protein folding and conformational stress in microbial cells producing recombinant proteins : a host comparative overview / Gasser, Brigitte (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Saloheimo, Markku (VTT Technical Research Centre (Espoo, Finlàndia)) ; Rinas, Ursula (Helmholtz Center for Infection Research (Braunschweig, Alemanya)) ; Dragosits, Martin (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Rodríguez-Carmona, Escarlata (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Baumann, Kristin (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Giuliani, Maria (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia)) ; Parrilli, Ermenegilda (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia)) ; Branduardi, Paola (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Milà, Itàlia)) ; Lang, Christine (Technical University Berlin. Institute for Microbiology and Genetics) ; Porro, Danilo (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Milà, Itàlia)) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Tutino, Maria Luisa (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia)) ; Mattanovich, Diethard (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. [...]
2008 - 10.1186/1475-2859-7-11
Microbial cell factories, Vol. 7, N. 11 (April 2008) , p. 1-18  
5.
14 p, 879.6 KB Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels / Carnicer Heras, Marc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Baumann, Kristin (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Töplitz, Isabelle (University of Applied Sciences. School of Bioengineering (Viena, Àustria)) ; Sánchez-Ferrando, Francisco (Universitat Autònoma de Barcelona. Departament de Química) ; Mattanovich, Diethard (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background: Analysis of the cell operation at the metabolic level requires collecting data of different types and to determine their confidence level. In addition, the acquired information has to be combined in order to obtain a consistent operational view. [...]
2009 - 10.1186/1475-2859-8-65
Microbial cell factories, Vol. 8, N. 65 (December 2009) , p. 1-14
5 documents
6.
15 p, 454.6 KB Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris / Baumann, Kristin (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Adelantado Vallvé, Núria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Lang, Christine (Technical University of Berlin. Department of Biotechnology) ; Mattanovich, Diethard (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Background: The increasing availability of 'omics' databases provide important platforms for yeast engineering strategies since they offer a lot of information on the physiology of the cells under diverse growth conditions, including environmental stresses. [...]
2011
Microbial cell factories, Vol. 10, N. 93 (November 2011) , p. 1-15
2 documents
7.
3 p, 123.0 KB Systems metabolic engineering, industrial biotechnology and microbial cell factories : Editorial / Lee, Sang Yup (Metabolic and Biomolecular Engineering National Research Laboratory (República de Corea)) ; Mattanovich, Diethard (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
2012 - 10.1186/1475-2859-11-156
Microbial cell factories, Vol. 11, N. 156 (December 2012) , p. 1-3  
8.
22 p, 1.3 MB A multi-level study of recombinant Pichia pastoris in different oxygen conditions / Baumann, Kristin (Universitat Autonoma de Barcelona. Departament d'Enginyeria Química) ; Albiol i Sala, Joan (Universitat Autonoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Carnicer, Marc (Universitat Autonoma de Barcelona. Departament d'Enginyeria Química) ; Dragosits, Martin (University of Natural Resources and Applied Life Sciences (Viena, Àustria)) ; Graf, Alexandra B. (University of Natural Resources and Applied Life Sciences (Viena, Àustria)) ; Stadlmann, Johannes (University of Natural Resources and Applied Life Sciences (Viena, Àustria)) ; Jouhten, Paula (VTT Technical Research Centre of Finland (Espoo, Finlàndia)) ; Maaheimo, Hannu (VTT Technical Research Centre of Finland (Espoo, Finlàndia)) ; Gasser, Brigitte (University of Natural Resources and Applied Life Sciences (Viena, Àustria)) ; Mattanovich, Diethard (University of Natural Resources and Applied Life Sciences (Viena, Àustria)) ; Ferrer Alegre, Pau (Universitat Autonoma de Barcelona. Departament d'Enginyeria Química)
Background: Yeasts are attractive expression platforms for many recombinant proteins, and there is evidence for an important interrelation between the protein secretion machinery and environmental stresses. [...]
2010
BMC Systems biology, Vol. 4, N. 141 (October 2010) , p. 1-22
7 documents
9.
16 p, 1.1 MB The impact of oxygen on the transcriptome of recombinant S. cervisiae and P. pastoris - a comparative analysis / Baumann, Kristin (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Dato, Laura (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Itàlia)) ; Graf, Alexandra B. (University of Natural Resources and Applied Life Sciences (Viena, Àustria)) ; Frascotti, Gianni (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Itàlia)) ; Dragosits, Martin (University of Natural Resources and Applied Life Sciences (Viena, Àustria)) ; Porro, Danilo (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Itàlia)) ; Mattanovich, Diethard (University of Natural Resources and Applied Life Sciences (Viena, Àustria)) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Branduardi, Paola (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Itàlia))
Background: Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins. Their known genome sequences enabled several transcriptomic profiling studies under many different environmental conditions, thus mimicking not only perturbations and adaptations which occur in their natural surroundings, but also in industrial processes. [...]
2011
BMC genomics, Vol. 12, N. 218 (May 2011) , p. 1-16
4 documents

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