Resultados globales: 2 registros encontrados en 0.02 segundos.
Artículos, Encontrados 2 registros
Artículos Encontrados 2 registros  
1.
7 p, 370.3 KB Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control / Platas, Gemma (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Rodríguez-Carmona, Escarlata (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; García Fruitós, Elena (CIBER de Bioingeniería, Biomateriales y Nanomedicina) ; Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Villaverde Corrales, Antonio (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Universitat Autònoma de Barcelona. Departament de Genètica i Microbiologia
The effects and effectiveness of the chaperone pair GroELS on the yield and quality of recombinant polypeptides produced in Escherichia coli are matter of controversy, as the reported activities of this complex are not always consistent and eventually indicate undesired side effects. [...]
2011 - 10.1186/1475-2859-10-79
Microbial cell factories, Vol. 10 (October 2011) , art. 79  
2.
11 p, 58.8 KB Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay / Toledo-Rubio, Verónica (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Vázquez Gómez, Esther, dir. (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Platas, Gemma (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Domingo i Espín, Joan (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Steinkamp, Elke (Bernhard-Nocht-Institut für Tropenmedizin) ; Garcia Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ferrer Miralles, Neus (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. [...]
2010
Journal of biomolecular screening, Vol. 15, No. 4 (April 2010) , p. 453-457
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