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21 p, 2.5 MB |
A systems biology framework integrating GWAS and RNA-seq to shed light on the molecular basis of sperm quality in swine
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Gòdia, Marta (Centre de Recerca en Agrigenòmica) ;
Reverter, Antonio (Queensland Bioscience Precinct. CSIRO Agriculture and Food (Australia)) ;
González Prendes, Rayner (Wageningen University & Research. Animal Breeding and Genomics (The Netherlands)) ;
Ramayo-Caldas, Yuliaxis (Institut de Recerca i Tecnologia Agroalimentàries. Genètica i Millora Animal) ;
Castelló, Anna (Centre de Recerca en Agrigenòmica) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Clop, Alex (Centre de Recerca en Agrigenòmica)
Background: Genetic pressure in animal breeding is sparking the interest of breeders for selecting elite boars with higher sperm quality to optimize ejaculate doses and fertility rates. However, the molecular basis of sperm quality is not yet fully understood. [...]
2020 -  10.1186/s12711-020-00592-0
Genetics, selection, evolution, Vol. 52 (December 2020) , art. 72
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2.
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Characterisation of sperm piRNAs and their correlation with semen quality traits in swine
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Ablondi, Michela (University of Parma. Department of Veterinary Science (Italy)) ;
Gòdia, Marta (Centre de Recerca en Agrigenòmica) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica) ;
Clop, Alex (Centre de Recerca en Agrigenòmica) ;
Universitat Autònoma de Barcelona.
Departament de Ciència Animal i dels Aliments
Piwi-interacting RNAs (piRNAs) are a class of non-coding RNAs that are essential in the transcriptional silencing of transposable elements and warrant genome stability in the mammalian germline. In this study, we have identified piRNAs in porcine sperm using male germline and zygote datasets from human, mice, cow and pig, and evaluated the relation between their abundances and sperm quality traits. [...]
2021 - 10.1111/age.13022
Animal Genetics, Vol. 52, Issue 1 (February 2021) , p. 114-120
3 documentos
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3.
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9 p, 319.1 KB |
Whole genome sequencing identifies allelic ratio distortion in sperm involving genes related to spermatogenesis in a swine model
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Gòdia, Marta (Centre de Recerca en Agrigenòmica) ;
Casellas Vidal, Joaquim (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Ruiz-Herrera Moreno, Aurora (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Rodríguez Gil, Joan E. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Castelló Farré, Anna (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Clop, Alex (Centre de Recerca en Agrigenòmica) ;
Universitat Autònoma de Barcelona.
Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia
Transmission Ratio Distortion (TRD), the uneven transmission of an allele from a parent to its offspring, can be caused by allelic differences affecting gametogenesis, fertilization or embryogenesis. [...]
2020 - 10.1093/dnares/dsaa019
DNA research, Vol. 27, Issue 5 (October 2020) , art. dsaa019
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4.
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A pilot RNA-seq study in 40 Pietrain ejaculates to characterize the porcine sperm microbiome
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Gòdia, Marta (Centre de Recerca en Agrigenòmica) ;
Ramayo-Caldas, Yuliaxis (Institut de Recerca i Tecnologia Agroalimentàries) ;
Zingaretti, Laura M. (Centre de Recerca en Agrigenòmica) ;
Darwich Soliva, Laila (Universitat Autònoma de Barcelona. Centre de Recerca en Sanitat Animal) ;
López, Samantha (Universitat de Barcelona. Departament de Física de la Matèria Condensada) ;
Rodríguez-Gil, Joan E. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Yeste Oliveras, Marc (Universitat de Girona. Institut de Tecnologia Agroalimentària) ;
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica) ;
Clop, Alex (Centre de Recerca en Agrigenòmica)
The microbiome plays a key role in homeostasis and health and it has been also linked to fertility and semen quality in several animal species including swine. Despite the more than likely importance of sperm bacteria on the boar's reproductive ability and the dissemination of pathogens and antimicrobial resistance genes, the high throughput characterization of the swine sperm microbiome remains scarce. [...]
2020 - 10.1016/j.theriogenology.2020.08.001
Theriogenology, Vol. 157 (November 2020) , p. 525-533
2 documentos
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5.
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11 p, 2.2 MB |
Identification of circular RNAs in porcine sperm and evaluation of their relation to sperm motility
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Gòdia, Marta (Centre de Recerca en Agrigenòmica) ;
Castelló Farré, Anna (Centre de Recerca en Agrigenòmica) ;
Rocco, Martina (Centre de Recerca en Agrigenòmica) ;
Cabrera Pane, Betlem (Centre de Recerca en Agrigenòmica) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Balasch, Sam (Grup Gepork S.A.) ;
Lewis, Craig (PIC Europe) ;
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica) ;
Clop, Alex (Centre de Recerca en Agrigenòmica) ;
Universitat Autònoma de Barcelona.
Departament de Ciència Animal i dels Aliments ;
Universitat Autònoma de Barcelona.
Departament de Medicina i Cirurgia Animals
Circular RNAs (circRNAs) are emerging as a novel class of noncoding RNAs which potential role as gene regulators is quickly gaining interest. circRNAs have been studied in different tissues and cell types across several animal species. [...]
2020 - 10.1038/s41598-020-64711-z
Scientific reports (Nature Publishing Group), Vol. 10 (May 2020) , art. 7985
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6.
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18 p, 1.6 MB |
Impact of intramammary inoculation of inactivated Lactobacillus rhamnosus and antibiotics on the milk microbiota of water buffalo with subclinical mastitis
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Catozzi, Carlotta (Università di Milano. Dipartimento di Medicina Veterinaria) ;
Cuscó, Anna (Vetgenomics) ;
Lecchi, Cristina (Università di Milano. Dipartimento di Medicina Veterinaria) ;
De Carlo, Esterina (Istituto Zooprofilattico Sperimentale del Mezzogiorno. National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions) ;
Vecchio, Domenico (Istituto Zooprofilattico Sperimentale del Mezzogiorno. National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions) ;
Martucciello, Alessandra (Istituto Zooprofilattico Sperimentale del Mezzogiorno. National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions) ;
D'Angelo, Luisa (Istituto Zooprofilattico Sperimentale del Mezzogiorno. National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions) ;
Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ;
Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ;
Ceciliani, Fabrizio (Università di Milano. Dipartimento di Medicina Veterinaria.)
Water buffalo mastitis represents a major issue in terms of animal health, cost of therapy, premature culling and decreased milk yeld. The emergence of antibiotic resistance has led to investigate strategies to avoid or reduce antibiotics' based therapies, in particular during subclinical mastitis. [...]
2019 - 10.1371/journal.pone.0210204
PloS one, Vol. 14 Núm. 1 (january 2019) , p. e0210204
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7.
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14 p, 1.0 MB |
A RNA-seq analysis to describe the boar sperm transcriptome and its seasonal changes
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Gòdia, Marta (Centre de Recerca en Agrigenòmica) ;
Estill, Molly (Wayne State University. Department of Obstetrics and Gynecology) ;
Castelló Farré, Anna (Centre de Recerca en Agrigenòmica) ;
Balasch, Sam (Grup Gepork S.A.) ;
Rodríguez-Gil, Joan E. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Krawetz, Stephen A. (Wayne State University. Department of Obstetrics and Gynecology) ;
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica) ;
Clop, Alex (Centre de Recerca en Agrigenòmica) ;
Universitat Autònoma de Barcelona.
Departament de Ciència Animal i dels Aliments
Understanding the molecular basis of cell function and ultimate phenotypes is crucial for the development of biological markers. With this aim, several RNA-seq studies have been devoted to the characterization of the transcriptome of ejaculated spermatozoa in relation to sperm quality and fertility. [...]
2019 - 10.3389/fgene.2019.00299
Frontiers in genetics, Vol. 10 (April 2019) , art. 299
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8.
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9.
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7 p, 1.4 MB |
Expression patterns and genetic variation of the ovine skeletal muscle transcriptome of sheep from five Spanish meat breeds
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Noce, Antonia (Centre de Recerca en Agrigenòmica) ;
Cardoso, Tainã Figueiredo (Centre de Recerca en Agrigenòmica) ;
Manunza, Arianna (Centre de Recerca en Agrigenòmica) ;
Martínez Martínez, Amparo (Universidad de Córdoba. Departamento de Genética) ;
Cánovas Tienda, Ángela (Institut de Recerca i Tecnologia Agroalimentàries) ;
Pons Barro, Agueda (Servei de Millora Agrària i Pesquera (SEMILLA)) ;
Bermejo Asensio, Luis Alberto (Universidad de la Laguna) ;
Landi, Vincenzo (Universidad de Córdoba. Departamento de Genética) ;
Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Jordana i Vidal, Jordi (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Delgado Bermejo, Juan Vicente (Universidad de Córdoba. Departamento de Genética) ;
Adán Belmonte, Silvia (Federación de Razas Autóctonas de Galicia (BOAGA)) ;
Capote, Juan (Instituto Canario de Investigaciones Agrarias) ;
Vidal i Fàbrega, Oriol (Universitat de Girona. Departament de Biologia) ;
Pazzola, Michele (University of Sassari. Department of Veterinary Medicine) ;
Vacca, Giuseppe Massimo (University of Sassari. Department of Veterinary Medicine) ;
Casellas Vidal, Joaquim (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Amills i Eras, Marcel (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
The goal of the current study is to analyse the gene expression profile of the ovine skeletal muscle as well as to characterize the genetic variation of transcripts expressed in such tissue. This aim has been achieved by sequencing the longissimus dorsi transcriptomes of 50 sheep distributed in five pools representing the Canaria de Pelo, Roja Mallorquina, Gallega, Xisqueta and Ripollesa Spanish autochthonous breeds. [...]
2018 - 10.1038/s41598-018-28760-9
Scientific reports (Nature Publishing Group), Vol. 8 (2018) , art. 10486
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10.
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15 p, 620.2 KB |
Endometrial gene expression profile of pregnant sows with extreme phenotypes for reproductive efficiency
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Córdoba, Sarai (Centre de Recerca en Agrigenòmica) ;
Balcells Ortega, Ingrid (Centre de Recerca en Agrigenòmica) ;
Castelló Farré, Anna (Centre de Recerca en Agrigenòmica) ;
Ovilo, Cristina (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria) ;
Noguera Jiménez, José Luis (Institut de Recerca i Tecnologia Agroalimentàries) ;
Timoneda i Heredia, Oriol (Centre de Recerca en Agrigenòmica) ;
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica) ;
Universitat Autònoma de Barcelona.
Departament de Genètica Animal
2015 0) and low (L, EBV 0) prolificacy phenotypes. For each sample, mRNA and small RNA libraries were RNA-sequenced, identifying 141 genes and 10 miRNAs differentially expressed between H and L groups. We selected four miRNAs based on their role in reproduction, and five genes displaying the highest differences and a positive mapping into known reproductive QTLs for RT-qPCR validation on the whole extreme population. Significant differences were validated for genes: PTGS2 (p = 0.03; H/L ratio = 3.50), PTHLH (p = 0.03; H/L ratio = 3.69), MMP8 (p = 0.01; H/L ratio = 4.41) and SCNN1G (p = 0.04; H/L ratio = 3.42). Although selected miRNAs showed similar expression levels between H and L groups, significant correlation was found between the expression level of ssc-miR-133a (p 0.01) and sscmiR-92a (p 0.01) and validated genes. These results provide a better understanding of the genetic architecture of prolificacy-related traits and embryo implantation failure in pigs - 10.1038/srep14416
Scientific reports (Nature Publishing Group), Vol. 5 (October 2015) , art. 14416
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