1.
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34 p, 614.1 KB |
Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?
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Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cámara, Elena (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The increasing demand for recombinant proteins for a wide range of applications, from biopharmaceutical protein complexes to industrial enzymes, is leading to important growth in this market. Among the different efficient host organism alternatives commonly used for protein production, the yeast Pichia pastoris (Komagataella phaffii) is currently considered to be one of the most effective and versatile expression platforms. [...]
2019 - 10.1016/j.nbt.2019.06.002
New Biotechnology, Vol. 53 (November 2019) , p. 24-34
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2.
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28 p, 460.0 KB |
Enzymatic production of biodiesel : Strategies to overcome methanol inactivation
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Lotti, Marina (Università degli Studi Milano-Bicocca. Dipartimento di Biotecnologie e Bioscienze) ;
Pleiss, Jürgen (University of Stuttgart. Institute of Biochemistry and Technical Biochemistry) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Lipase-catalyzed transesterification of triglycerides and alcohols to obtain biodiesel is an environmentally friendly and sustainable route for fuels production since, besides proceeding in mild reaction conditions, it allows for the use of low-cost feedstocks that contain water and free fatty acids, for example non-edible oils and waste oils. [...]
2018 - 10.1002/biot.201700155
Biotechnology Journal, Vol. 13, Issue 5 (May 2018) , art. 1700155
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3.
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24 p, 1.6 MB |
Recent advances in lipases and their applications in the food and nutraceutical industry
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Reyes-Reyes, Ana Laura (Center for Research and Assistance in Technology and Design of the State of Jalisco (Mexico)) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Sandoval, Georgina (Center for Research and Assistance in Technology and Design of the State of Jalisco (Mexico))
Lipases are efficient enzymes with promising applications in the nutraceutical and food industry, as they can offer high yields, pure products under achievable reaction conditions, and are an environmentally friendly option. [...]
2022 - 10.3390/catal12090960
Catalysts, Vol. 12, Issue 9 (September 2022) , art. 960
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4.
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13 p, 1.7 MB |
Producing natural flavours from isoamyl alcohol and fusel oil by using immobilised Rhizopus oryzae lipase
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Sebastian, Xavier (Hausmann, S.L.) ;
Bueno, Jose María (Hausmann, S.L.) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Enzymatic synthesis of short-chain esters (flavours) might enable their labelling as natural, increasing their value. Covalently immobilised Rhizopus oryzae lipase (EO-proROL) was used to synthesise isoamyl butyrate and acetate. [...]
2022 - 10.3390/catal12060639
Catalysts, Vol. 12, Issue 6 (June 2022) , art. 639
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5.
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10 p, 4.6 MB |
Near Infrared Spectroscopy : A useful technique for inline monitoring of the enzyme catalyzed biosynthesis of third-generation biodiesel from waste cooking oil
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Moya, Desireè (Universitat Autònoma de Barcelona. Departament de Química) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Alcalà Cañadas, Ma. del Mar (Universitat Autònoma de Barcelona. Departament de Química)
Near Infrared (NIR) spectroscopy was used for inline monitoring the enzymatic production of third-generation biodiesel from waste cooking oil (WCO) -with fatty acid profile similar to olive oil- under the principles of Continued Process Verification (CPV) and Process Analytical Technology (PAT). [...]
2022 - 10.1016/j.fuel.2022.123794
Fuel, Vol. 319 (July 2022) , art. 123794
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6.
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12 p, 1.8 MB |
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
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Gasset Franch, Arnau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Garrigos, Javier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The combination of strain and bioprocess engineering strategies should be considered to obtain the highest levels of recombinant protein production (RPP) while assuring product quality and process reproducibility of heterologous products. [...]
2022 - 10.3389/fbioe.2022.818434
Frontiers in Bioengineering and Biotechnology, Vol. 10 (January 2022) , art. 818434
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7.
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13 p, 1.3 MB |
Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9
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Garrigos, Javier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Weninger, Astrid (Graz University of Technology) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Schmid, Christian (Graz University of Technology) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Rinnofner, Claudia (Austrian Centre of Industrial Biotechnology (ACIB)) ;
Glieder, Anton (Graz University of Technology) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Currently, the numerous and versatile applications in pharmaceutical and chemical industry make the recombinant production of cytochrome P450 enzymes (CYPs) of great biotechnological interest. Accelerating the drug development process by simple, quick and scalable access of human drug metabolites is key for efficient and targeted drug development in response to new and sometimes unexpected medical challenges and needs. [...]
2021 - 10.1186/s12934-021-01577-4
Microbial cell factories, Vol. 20 (April 2021) , art. 90
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8.
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12 p, 1.5 MB |
Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris
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Garrigos, Javier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Vuoristo, Kiira (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Nieto-Taype, Miguel Angel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Tähtiharju, Juha (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Uusitalo, Jaana (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Tukiainen, Pauliina (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Schmid, Christian (Bisy GmbH, Wuenschendorf 292, 8200 Hofstaetten/Raab, Austria) ;
Tolstorukov, Ilya (Keck Graduate Institute) ;
Madden, Knut (Biogrammatics Inc, 2794 Loker Ave, West, Suite 103, Carlsbad, CA 92010 USA) ;
Penttilä, Merja (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Glieder, Anton (Bisy GmbH, Wuenschendorf 292, 8200 Hofstaetten/Raab, Austria) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Pichia pastoris is a powerful and broadly used host for recombinant protein production (RPP), where past bioprocess performance has often been directed with the methanol regulated AOX1 promoter (P), and the constitutive GAP promoter (P). [...]
2021 - 10.1186/s12934-021-01564-9
Microbial cell factories, Vol. 20 (march 2021)
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9.
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15 p, 900.6 KB |
Constitutive expression in komagataella phaffii of mature rhizopus oryzae lipase jointly with its truncated prosequence improves production and the biocatalyst operational stability
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López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Rhizopus oryzae lipase (ROL) containing 28 C-terminal amino acids of the prosequence fused to the N-terminal mature sequence in ROL (proROL) was successfully expressed in the methylotrophic yeast Komagataella phaffii (Pichia pastoris) under the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP). [...]
2021 - 10.3390/catal11101192
Catalysts, Vol. 11, Issue 10 (October 2021) , art. 1192
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10.
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21 p, 2.7 MB |
Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
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Nieto-Taype, Miguel Angel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is currently considered one of the most promising hosts for recombinant protein production (RPP) and metabolites due to the availability of several tools to efficiently regulate the recombinant expression, its ability to perform eukaryotic post-translational modifications and to secrete the product in the extracellular media. [...]
2020 - 10.3389/fbioe.2020.00632
Frontiers in Bioengineering and Biotechnology, Vol. 8 (June 2020) , art. 632
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