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Articles, 2 records found

Articles	2 records found

15 p, 1.7 MB

Selecting subpopulations of high-quality protein conformers among conformational mixtures of recombinant bovine MMP-9 solubilized from inclusion bodies / Carratalá, José Vicente (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Gifre-Renom, Laia (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Producció de Remugants) ; Roca-Pinilla, Ramon (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Producció de Remugants) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Producció de Remugants) ; Garcia-Fruitos, Elena (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Producció de Remugants) ; Sanchez, Julieta M. (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies-IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories Clearcoli (an engineered form of Escherichia coli) and Lactococcus lactis, mainly forming part of IBs and partially recovered under non-denaturing conditions. [...]
2021 - DOI (Digital Object Identifier)

10.3390/ijms22063020
International journal of molecular sciences, Vol. 22, Issue 6 (March 2021) , art. 3020

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11 p, 58.8 KB

Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay / Toledo-Rubio, Verónica (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Vázquez Gómez, Esther, dir. (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Platas, Gemma (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Domingo-Espin, Joan (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Steinkamp, Elke (Bernhard-Nocht-Institut für Tropenmedizin) ; Garcia-Fruitos, Elena (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. [...]
2010 - DOI (Digital Object Identifier)

10.1177/1087057110363911
Journal of biomolecular screening, Vol. 15, No. 4 (April 2010) , p. 453-457

3 documents

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