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1.
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9 p, 2.5 MB |
A novel ratiometric fluorescent approach for the modulation of the ydnamic range of lateral flow immunoassays
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Sena-Torralba, Amadeo (Institut Català de Nanociència i Nanotecnologia) ;
Torné-Morató, Helena (Institut Català de Nanociència i Nanotecnologia) ;
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia) ;
Ranjbar, Saba (Institut Català de Nanociència i Nanotecnologia) ;
Nejad, Mohammad Amin Farahmand (Institut Català de Nanociència i Nanotecnologia) ;
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia) ;
Idili, Andrea (Institut Català de Nanociència i Nanotecnologia) ;
Hormozi-Nezhad, M.R. (Sharif University of Technology. Department of Chemistry) ;
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)
The majority of lateral flow assays (LFAs) use single-color optical labels to provide a qualitative naked-eye detection, however this detection method displays two important limitations. First, the use of a single-color label makes the LFA prone to results misinterpretation. [...]
2022 -  10.1002/admt.202101450
Advanced Materials Technologies, Vol. 7, issue 8 (August 2022) , art. 2101450
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2.
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32 p, 1.2 MB |
Biotinylated phosphorus dendrimers as control line in nucleic acid lateral flow tests
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Vidal, Laura (Universitat Autònoma de Barcelona. Departament de Química) ;
Ben Aissa Soler, Alejandra (Universitat Autònoma de Barcelona. Departament de Química) ;
Salabert Sabaté, Jordi (Universitat Autònoma de Barcelona. Departament de Química) ;
Jara, José Juan (Universitat Autònoma de Barcelona. Departament de Química) ;
Vallribera Massó, Adelina (Universitat Autònoma de Barcelona. Departament de Química) ;
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química) ;
Sebastián Pérez, Rosa Maria (Universitat Autònoma de Barcelona. Departament de Química)
Lateral flow assays (LFA) are an affordable, easy-to-use, qualitative rapid test for clinical diagnosis in nonlaboratory environments and low-resource facilities. The control line of these tests is very important to provide a valid result, confirming that the platform operates correctly. [...]
2020 - 10.1021/acs.biomac.0c00161
Biomacromolecules, Vol. 21, Issue 3 (March 2020) , p. 1315-1323
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3.
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23 p, 7.2 MB |
Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens
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Ben Aissa Soler, Alejandra (Universitat Autònoma de Barcelona. Departament de Química) ;
Jara, José Juan (Universitat Autònoma de Barcelona. Departament de Química) ;
Sebastián Pérez, Rosa Maria (Universitat Autònoma de Barcelona. Departament de Química) ;
Vallribera Massó, Adelina (Universitat Autònoma de Barcelona. Departament de Química) ;
Campoy Sánchez, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química)
Due to the increasing need of rapid tests for application in low resource settings, WHO summarized their ideal features under the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, Delivered to those who need it). [...]
2017 - 10.1016/j.bios.2016.08.046
Biosensors & bioelectronics, Vol. 88 (February 2017) , p. 265-272
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4.
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6 p, 1.7 MB |
Selection and characterisation of bioreceptors to develop nanoparticle-based lateral-flow immunoassays in the context of the SARS-CoV-2 outbreak
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Hu, Liming (Institut Català de Nanociència i Nanotecnologia) ;
Calucho, Enric (Institut Català de Nanociència i Nanotecnologia) ;
Fuentes-Chust, Celia (Institut Català de Nanociència i Nanotecnologia) ;
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia) ;
Idili, Andrea (Institut Català de Nanociència i Nanotecnologia) ;
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia) ;
Rivas, Lourdes (Institut Català de Nanociència i Nanotecnologia) ;
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)
This manuscript aims at raising the attention of the scientific community to the need for better characterised bioreceptors for fast development of point-of-care diagnostic devices able to support mass frequency testing. [...]
2022 - 10.1039/d2lc00486k
Lab on a chip, Vol. 22, issue 16 (Aug. 2022) , p. 2938-2943
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5.
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13 p, 2.6 MB |
Immunomagnetic separation improves the detection of mycobacteria by paper-based lateral and vertical flow immunochromatographic assays
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Ben Aissa Soler, Alejandra (Universitat Autònoma de Barcelona. Departament de Química) ;
Julián Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Zanoni, Maria Valnice (São Paulo State University. Department of Analytical Chemistry) ;
Pividori, María Isabel (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
This work addresses a method that combines immunomagnetic separation (IMS) and paperbased nucleic acid immunochromatographic assay for the sensitive detection of Mycolicibacterium fortuitum (basonym Mycobacterium fortuitum) In particular, the preconcentration of the bacteria was achieved by using magnetic particles modified with an antibody specific towards mycobacteria. [...]
2021 - 10.3390/s21185992
Sensors (Basel, Switzerland), Vol. 21, Issue 18 (September 2021) , art. 5992
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6.
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12 p, 1.0 MB |
A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
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Tang, Xiaoqian (Université de Perpignan Via Domitia) ;
Zhang, Qi (Ministry of Agriculture and Rural Affairs. Quality Inspection and Test Center for Oilseeds Products) ;
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química) ;
Zhang, Zhaowei (Ministry of Agriculture and Rural Affairs. Laboratory of Risk Assessment for Oilseeds Products) ;
Marty, Jean-Louis (Sensbiotech) ;
Catanante, Gaëlle (Université de Perpignan Via Domitia)
Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. [...]
2022 - 10.3390/bios12020059
Biosensors, Vol. 12, Issue 2 (January 2022) , art. 59
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7.
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8.
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10 p, 2.8 MB |
Paper-based electrophoretic bioassay : biosensing in whole blood operating via smartphone
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Sena Torralba, Amadeo (Institut Català de Nanociència i Nanotecnologia) ;
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia) ;
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia) ;
Torné-Morató, Helena (Institut Català de Nanociència i Nanotecnologia) ;
Müller, Alexander (Institut Català de Nanociència i Nanotecnologia) ;
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)
Point-of-care (PoC) tests are practical and effective diagnostic solutions for major clinical problems, ranging from the monitoring of a pandemic to recurrent or simple measurements. Although, in recent years, a great improvement in the analytical performance of such sensors has been observed, there is still a major issue that has not been properly solved: The ability to perform adequate sample treatments. [...]
2021 - 10.1021/acs.analchem.0c04330
Analytical chemistry, Vol. 93, issue 6 (Feb. 2021) , p. 3112-3121
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9.
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11 p, 1.5 MB |
Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay
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Porras, Juan Carlos (Universitat Autònoma de Barcelona. Departament de Química) ;
Bernuz, Mireia (Universitat Autònoma de Barcelona. Departament de Química) ;
Marfa, Jennifer (Universitat Autònoma de Barcelona. Departament de Química) ;
Pallarès-Rusiñol, Arnau (Universitat Autònoma de Barcelona. Departament de Química) ;
Martí, Mercè (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química)
A lateral flow assay (LFA) is a paper-based, point-of-need test designed to detect a specific analyte in complex samples in low-resource settings. Although LFA has been successfully used in different applications, its use is still limited when high sensitivity is required, especially in the diagnosis of an early-stage condition. [...]
2021 - 10.3390/nano11030741
Nanomaterials, Vol. 11, Núm. 3 (March 2021) , art. 741
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10.
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14 p, 946.3 KB |
Lateral flow assay modified with time-delay wax barriers as a sensitivity and signal enhancement strategy
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Sena Torralba, Amadeo (Institut Català de Nanociència i Nanotecnologia) ;
Ngo, Duy Ba (King Mongkut's University of Technology Thonburi. School of Bioresources and Technology) ;
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia) ;
Hu, Liming (Institut Català de Nanociència i Nanotecnologia) ;
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia) ;
Bergua Canudo, José Francisco (Institut Català de Nanociència i Nanotecnologia) ;
Rosati, Giulio (Institut Català de Nanociència i Nanotecnologia) ;
Surareungchai, Werasak (King Mongkut's University of Technology Thonburi. Nanoscience and Nanotechnology Graduate Research Program) ;
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)
The ease of use, low cost and quick operation of lateral flow assays (LFA) have made them some of the most common point of care biosensors in a variety of fields. However, their generally low sensitivity has limited their use for more challenging applications, where the detection of low analytic concentrations is required. [...]
2020 - 10.1016/j.bios.2020.112559
Biosensors & bioelectronics, Vol. 168 (Nov. 2020) , art. 112559
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