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1.
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16 p, 1.9 MB |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter
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Bernat Camps, Núria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ebner, Katharina (Bisy GmbH) ;
Schusterbauer, Veronika (Bisy GmbH) ;
Fischer, Jasmin Elgin (Bisy GmbH) ;
Nieto-Taype, Miguel Angel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Glieder, Anton (Bisy GmbH) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of Komagataella phaffii (Pichia pastoris) can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-product from the biodiesel industry, while producing high- and low-added value products. [...]
2023 -  10.3389/fbioe.2023.1130583
Frontiers in Bioengineering and Biotechnology, Vol. 11 (March 2023) , art. 1130583
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2.
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34 p, 614.1 KB |
Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?
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Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cámara, Elena (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The increasing demand for recombinant proteins for a wide range of applications, from biopharmaceutical protein complexes to industrial enzymes, is leading to important growth in this market. Among the different efficient host organism alternatives commonly used for protein production, the yeast Pichia pastoris (Komagataella phaffii) is currently considered to be one of the most effective and versatile expression platforms. [...]
2019 - 10.1016/j.nbt.2019.06.002
New Biotechnology, Vol. 53 (November 2019) , p. 24-34
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3.
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40 p, 1.6 MB |
Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter
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Cámara, Elena (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Monforte, Sergi (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The methanol-regulated alcohol oxidase promoter (PAOX1) of Pichia pastoris (syn. Komagataella spp. ) is one of the strongest promoters for heterologous gene expression. Although increasing the gene dosage is a common strategy to improve recombinant protein productivities, P. [...]
2019 - 10.1002/bit.26947
Biotechnology and Bioengineering, Vol. 116, Issue 7 (July 2019) , p. 1710-1720
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4.
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16 p, 2.4 MB |
Carbohydrate hydrolytic potential and redundancy of an anaerobic digestion microbiome exposed to acidosis, as uncovered by metagenomics
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Bertucci, Marie (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Calusinska, Magdalena (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Goux, Xavier (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Rouland-Lefèvre, Corinne (Sorbonne Universités. Institut de Recherche Développement. Institut d'Ecologie et des Sciences de l'Environnement) ;
Untereiner, Boris (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Ferrer, Pau (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Gerin, Patrick A. (Université Catholique de Louvain. Laboratory of Bioengineering, Earth) ;
Delfosse, Philippe (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department)
Increased hydrolysis of easily digestible biomass may lead to acidosis of anaerobic reactors and decreased methane production. Previously, it was shown that the structure of microbial communities changed during acidosis; however, once the conditions are back to optimal, biogas (initially CO2) production quickly restarts. [...]
2019 - 10.1128/AEM.00895-19
Applied and Environmental Microbiology, Vol. 85, Issue 15 (August 2019) , p. e00895-19
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5.
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11 p, 1.6 MB |
Process intensification at the expression system level for the production of 1-phosphate aldolase in antibiotic-free E. coli fed-batch cultures
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Pasini, Martina (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Fernández-Castané, Alfred (Aston University. Aston Institute of Materials Research) ;
Caminal i Saperas, Glòria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Overton, Tim W. (University of Birmingham. College of Engineering and Physical Sciences) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
To successfully design expression systems for industrial biotechnology and biopharmaceutical applications; plasmid stability, efficient synthesis of the desired product and the use of selection markers acceptable to regulatory bodies are of utmost importance. [...]
2022 - 10.1093/jimb/kuac018
Journal of Industrial Microbiology and Biotechnology, Vol. 49, Issue 4 (July 2022) , art. kuac018
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6.
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12 p, 1.5 MB |
Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris
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Garrigos, Javier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Vuoristo, Kiira (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Nieto-Taype, Miguel Angel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Tähtiharju, Juha (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Uusitalo, Jaana (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Tukiainen, Pauliina (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Schmid, Christian (Bisy GmbH, Wuenschendorf 292, 8200 Hofstaetten/Raab, Austria) ;
Tolstorukov, Ilya (Keck Graduate Institute) ;
Madden, Knut (Biogrammatics Inc, 2794 Loker Ave, West, Suite 103, Carlsbad, CA 92010 USA) ;
Penttilä, Merja (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Glieder, Anton (Bisy GmbH, Wuenschendorf 292, 8200 Hofstaetten/Raab, Austria) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Pichia pastoris is a powerful and broadly used host for recombinant protein production (RPP), where past bioprocess performance has often been directed with the methanol regulated AOX1 promoter (P), and the constitutive GAP promoter (P). [...]
2021 - 10.1186/s12934-021-01564-9
Microbial cell factories, Vol. 20 (march 2021)
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7.
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8.
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62 p, 1.5 MB |
Bacterial inclusion bodies are industrially exploitable amyloids
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de Marco, Ario (University of Nova Gorica. Laboratory for Environmental and Life Sciences (Slovenia)) ;
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Garcia-Fruitos, Elena (Institut de Recerca i Tecnologia Agroalimentàries) ;
Mitraki, Anna (University of Crete. Department of Materials Science and Technology (Greece)) ;
Peternel, Spela (Lupinica (Slovenia)) ;
Rinas, Ursula (Leibniz University of Hannover. Technical Chemistry and Life Science (Germany)) ;
Trujillo-Roldán, Mauricio A. (Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas) ;
Valdez-Cruz, Norma A. (Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas) ;
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Universitat Autònoma de Barcelona.
Departament de Genètica i de Microbiologia
Understanding the structure, functionalities and biology of functional amyloids is an issue of emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria during protein production processes, have emerged as unanticipated, highly tunable models for the scrutiny of the physiology and architecture of functional amyloids. [...]
2019 - 10.1093/femsre/fuy038
FEMS Microbiology Reviews, Vol. 43, Issue 1 (January 2019) , p. 53-72
7 documents
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9.
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15 p, 2.0 MB |
The effect of hypoxia on the lipidome of recombinant Pichia pastoris
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Adelantado Vallvé, Núria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Tarazona, Pablo (Georg-August University. Albrecht-von-Haller-Institute for Plant Sciences) ;
Grillitsch, Karlheinz (Austrian Centre of Industrial Biotechnology (ACIB)) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Monforte, Sergi (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Feussner, Ivo (Georg-August University. Albrecht-von-Haller-Institute for Plant Sciences) ;
Daum, Günther (Graz University of Technology. Institute of Biochemistry) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Cultivation of recombinant Pichia pastoris (Komagataella sp. ) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expression, mainly due to upregulation of glycolytic conditions. [...]
2017 - 10.1186/s12934-017-0699-4
Microbial cell factories, Vol. 16 (May 2017) , art. 86
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10.
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7 p, 370.3 KB |
Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control
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Platas, Gemma (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Rodríguez-Carmona, Escarlata (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Garcia-Fruitos, Elena (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) ;
Cano-Garrido, Olivia (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Universitat Autònoma de Barcelona.
Departament de Genètica i de Microbiologia
The effects and effectiveness of the chaperone pair GroELS on the yield and quality of recombinant polypeptides produced in Escherichia coli are matter of controversy, as the reported activities of this complex are not always consistent and eventually indicate undesired side effects. [...]
2011 - 10.1186/1475-2859-10-79
Microbial cell factories, Vol. 10 (October 2011) , art. 79
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