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Articles, 17 records found
Articles 17 records found  1 - 10next  jump to record:
1.
17 p, 1.6 MB Complex stability and dynamic subunit interchange modulates the disparate activities of the yeast moonlighting proteins Hal3 and Vhs3 / Abrie, J. Albert (Stellenbosch University. Department of Biochemistry) ; Molero Merinero, Cristina (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular) ; Ariño Carmona, Joaquín (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular) ; Strauss, Erick (Stellenbosch University. Department of Biochemistry) ; Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí"
Saccharomyces cerevisiae Hal3 and Vhs3 are moonlighting proteins, acting both as inhibitors of the serine/threonine protein phosphatase Ppz1 and as subunits (together with Cab3) of the unique heterotrimeric phosphopantothenoylcysteine decarboxylase (PPCDC) enzyme of Hemiascomycetous yeast. [...]
2015 - 10.1038/srep15774
Scientific reports, Vol. 5 (October 2015) , art. 15774  
2.
15 p, 2.0 MB The effect of hypoxia on the lipidome of recombinant Pichia pastoris / Adelantado Vallvé, Núria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Tarazona, Pablo (Georg-August University. Albrecht-von-Haller-Institute for Plant Sciences) ; Grillitsch, Karlheinz (Austrian Centre of Industrial Biotechnology (ACIB)) ; Garcia Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Monforte, Sergi (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Feussner, Ivo (Georg-August University. Albrecht-von-Haller-Institute for Plant Sciences) ; Daum, Günther (Graz University of Technology. Institute of Biochemistry) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Cultivation of recombinant Pichia pastoris (Komagataella sp. ) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expression, mainly due to upregulation of glycolytic conditions. [...]
2017 - 10.1186/s12934-017-0699-4
Microbial cell factories, Vol. 16 (May 2017) , art. 86  
3.
7 p, 1.2 MB Recombinant pharmaceuticals from microbial cells : a 2015 update / Sanchez-Garcia, Laura (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Martín, Lucas (Universitat Autònoma de Barcelona. Technology Transfer Office) ; Mangues, Ramon (Institut d'Investigacions Biomèdiques Sant Pau) ; Ferrer Miralles, Neus (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Vázquez Gómez, Esther (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona)) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Diabetes, growth or clotting disorders are among the spectrum of human diseases related to protein absence or malfunction. Since these pathologies cannot be yet regularly treated by gene therapy, the administration of functional proteins produced ex vivo is required. [...]
2016 - 10.1186/s12934-016-0437-3
Microbial cell factories, Vol. 15, Issue 33 (February 2016)  
4.
7 p, 370.3 KB Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control / Platas, Gemma (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Rodríguez-Carmona, Escarlata (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; García Fruitós, Elena (CIBER de Bioingeniería, Biomateriales y Nanomedicina) ; Cano Garrido, Olivia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Villaverde Corrales, Antonio (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Universitat Autònoma de Barcelona. Departament de Genètica i Microbiologia
The effects and effectiveness of the chaperone pair GroELS on the yield and quality of recombinant polypeptides produced in Escherichia coli are matter of controversy, as the reported activities of this complex are not always consistent and eventually indicate undesired side effects. [...]
2011 - 10.1186/1475-2859-10-79
Microbial cell factories, Vol. 10 (October 2011) , art. 79  
5.
35 p, 1.1 MB Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria. / Pesarrodona Roches, Mireia (Universitat Autònoma de Barcelona. Department de Genètica i de Microbiologia) ; Fernández, Yolanda (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona)) ; Foradada, Laia (Hospital Vall d'Hebron. CIBBIM-Nanomedicine) ; Sánchez Chardi, Alejandro (Universitat Autònoma de Barcelona. Servei de Microscòpia) ; Conchillo-Solé, Oscar (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Xu, Zhikun (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona)) ; Roldán, Mónica (Universitat Autònoma de Barcelona. Servei de Microscòpia) ; Villegas Hernández, Sandra (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular) ; Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Schwartz, Simó (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona)) ; Rinas, Ursula (Leibniz University of Hannover. Technical Chemistry & Life Science) ; Daura i Ribera, Xavier (Institució Catalana de Recerca i Estudis Avançats) ; Abasolo, Ibane (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona)) ; Vázquez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Biofabrication is attracting interest as a means to produce nanostructured functional materials because of its operational versatility and full scalability. Materials based on proteins are especially appealing, as the structure and functionality of proteins can be adapted by genetic engineering. [...]
2016
Biofabrication, Vol. 8 Núm 2 (Abril 2016) , p. 25001  
6.
38 p, 1.1 MB BBB-targeting, protein-based nanomedicines for drug and nucleic acid delivery to the CNS / Peluffo, Hugo (Institut Pasteur de Montevideo. Neuroinflammation Gene Therapy Laboratory) ; Unzueta Elorza, Ugutz (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Negro, Luciana (Institut Pasteur de Montevideo. Neuroinflammation Gene Therapy Laboratory,) ; Xu, Zhikun (CIBER en Bioingeniería, Biomateriales y Nanomedicina) ; Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
The increasing incidence of diseases affecting the central nervous system (CNS) demands the urgent development of efficient drugs. While many of these medicines are already available, the Blood Brain Barrier and to a lesser extent, the Blood Spinal Cord Barrier pose physical and biological limitations to their diffusion to reach target tissues. [...]
2015 - 10.1016/j.biotechadv.2015.02.004
Biotechnology advances, Vol. 33 Núm. 2(Març-Abril 2015) , p. 277-287
5 documents
7.
20 p, 248.7 KB A novel bio-functional material based on mammalian cell aggresomes / Rodríguez-Carmona, Escarlata (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Mendoza, Rosa (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ruiz Cánovas, Eugènia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Abasolo, Ibane (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona)) ; Schwartz Jr, Simó (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona)) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the physiological roles of these protein clusters and the functional status of the embedded proteins remain basically unexplored. [...]
2015 - 10.1007/s00253-015-6684-0
Applied microbiology and biotechnology, Vol. 99 Issue 17, p. 7079–7088 (Setembre 2015)
8 documents
8.
24 p, 1.7 MB Integration and Validation of the GenomeScale Metabolic Models of Pichia pastoris : a Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism / Tomàs Gamisans, Màrius (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Motivation: Genome-scale metabolic models (GEMs) are tools that allow predicting a phenotype from a genotype under certain environmental conditions. GEMs have been developed in the last ten years for a broad range of organisms, and are used for multiple purposes such as discovering new properties of metabolic networks, predicting new targets for metabolic engineering, as well as optimizing the cultivation conditions for biochemicals or recombinant protein production. [...]
2016 - 10.1371/journal.pone.0148031
PloS one, Vol. 11 Núm. 1 (January 2016) , p. 1-24  
9.
16 p, 2.3 MB Analysis of Two Putative Candida albicans Phosphopantothenoylcysteine Decarboxylase / Protein Phosphatase Z Regulatory Subunits Reveals an Unexpected Distribution of Functional Roles / Petrényi, Katalin (University of Debrecen. Department of Medical Chemistry) ; Molero Merinero, Cristina (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular) ; Kónya, Zoltán (University of Debrecen. Department of Medical Chemistry) ; Erdődi, Ferenc (University of Debrecen. Department of Medical Chemistry) ; Ariño Carmona, Joaquín (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular) ; Dombrádi, Viktor (University of Debrecen. Department of Medical Chemistry)
Protein phosphatase Z (Ppz) is a fungus specific enzyme that regulates cell wall integrity, cation homeostasis and oxidative stress response. Work on Saccharomyces cerevisiae has shown that the enzyme is inhibited by Hal3/Vhs3 moonlighting proteins that together with Cab3 constitute the essential phosphopantothenoylcysteine decarboxylase (PPCDC) enzyme. [...]
2016 - 10.1371/journal.pone.0160965
PloS one, Vol. 11 Núm. 8 (August 2016) , p. 1-16  
10.
19 p, 492.8 KB Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris / Jordà Murria, Joel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Cueto Rojas, Hugo (Delft University of Technology. Department of Biotechnology) ; Carnicer Heras, Marc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Aljoscha Wahl (Delft University of Technology. Department of Biotechnology) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Albiol i Sala, Joan (Universitat Autonoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of the production of a Rhizopus oryzae lipase (Rol) on P. [...]
2014 - 10.3390/metabo4020281
Metabolites, Vol. 4 (2014) , p. 281-299  

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