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5 p, 66.8 KB |
Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes
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Fernández-Torres, Belkys (Universitat Rovira i Virgili. Unitat de Microbiologia) ;
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona Departament de Sanitat i d'Anatomia Animals) ;
Carrillo-Muñoz, Alfonso J. (Asesoría Científica y de Investigación Aplicada) ;
Esteban, Alexandre (Universitat Autònoma de Barcelona Departament de Sanitat i d'Anatomia Animals) ;
Inza, Isabel (Universitat Rovira i Virgili. Unitat de Microbiologia) ;
Abarca Salat, Ma. Lourdes (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Guarro Artigas, Josep (Universitat de Barcelona. Càtedra de Microbiologia)
A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility.
of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 103 and 104 CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37°C) on intra- and interlaboratory.
agreement were analyzed. The optimal testing conditions identified were an inoculum of 104 CFU/ml, a.
temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.
2002
Journal of clinical microbiology, Vol. 40 Núm. 11 (Novembre 2002) , p. 3999-4003
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7 p, 385.6 KB |
Secondary metabolite profiling, growth profiles and other tools for species recognition and important Aspergillus mycotoxins
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Frisvad, J.C. (Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark) ;
Larsen, T.O. (Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark) ;
de Vries, R. (Microbiology, Utrecht University) ;
Meijer, M. (Microbiology, Utrecht University) ;
Houbraken, J. (CBS Fungal Biodiversity Centre) ;
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Ehrlich, K. (Southern Regional Research Center/ARS/USDA) ;
Samson, R.A. (CBS Fungal Biodiversity Centre)
Species in the genus Aspergillus have been classified primarily based on morphological features. Sequencing of house-hold genes has also been used in Aspergillus taxonomy and phylogeny, while extrolites and physiological features have been used less frequently. [...]
2007
Studies in Mycology, Vol. 59 (2007) , p. 31-37
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15 p, 1.4 MB |
Phylogeny of chrysosporia infecting reptiles : proposal of the new family Nannizziopsiaceae and five new species
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Stchigel, A.M. (Universitat Rovira i Virgili. Unitat de Micologia) ;
Sutton, D.A. (University of Texas Health Science Center, Department of Pathology) ;
Cano-Lira, J.F. (Universitat Rovira i Virgili. Unitat de Micologia) ;
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Abarca Salat, Ma. Lourdes (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Tintelnot, K. (Robert Koch Institute) ;
Wickes, B.L. (Texas Veterinary Medical Diagnostic Laboratory) ;
García, D. (Mycology Unit, University Rovira i Virgili) ;
Guarro, J. (Mycology Unit, University Rovira i Virgili)
We have performed a phenotypic and phylogenetic study of a set of fungi, mostly of veterinary origin, morphologically similar to the Chrysosporium asexual morph of Nannizziopsis vriesii (Onygenales, Eurotiomycetidae,Eurotiomycetes, Ascomycota). [...]
2013
Persoonia, Vol. 31 (Juny 2013) , p. 86-100
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7 p, 976.0 KB |
Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
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Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Castellà Gómez, Gemma (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Bragulat Arará, M. Rosa (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Sanseverino, Walter (Centre de Recerca en Agrigenòmica) ;
Sánchez, Armand (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular) ;
Aiese-Cigliano, Riccardo (Sequentia Biotech SL)
In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. [...]
2015
Scientific Reports, Març 2015, p. 1-7
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13 p, 5.4 MB |
Characterization of the species Malassezia pachydermatis and re-evaluation of its lipid dependence using a synthetic agar medium
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Castellà Gómez, Gemma (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Bragulat Arará, M. Rosa (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Puig Carles, Laura (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
The genus Malassezia includes lipophilic yeasts, which are part of the skin microbiota of various mammals and birds. Unlike the rest of Malassezia species, M. pachydermatis is described as non-lipid-dependent, as it is able to grow on Sabouraud glucose agar (SGA) without lipid supplementation. [...]
2017
Plos one, Juny 2017, p. 1-12
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7 p, 152.7 KB |
Molecular Analysis of Malassezia sympodialis-Related Strains from Domestic Animals
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Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Castellà Gómez, Gemma (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Hernández Escareño, Jesús Jaime (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Recently, several new lipid-dependent species belonging to the genus Malassezia have been described. Some of them, such as Malassezia dermatis, Malassezia nana, and the tentatively named “Malassezia equi,” have similar phenotypes and are genetically close to Malassezia sympodialis Simmons et Gue´ho 1990. [...]
2005
Journal of Clinical Microbiology, Vol. 43 Núm. 1 (Gener 2005) , p. 277-283
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4 p, 672.5 KB |
Dyes As Fungal Inhibitors : effect on Colony Diameter
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Bruguera Estrada, Mª Teresa (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Abarca Salat, Ma. Lourdes (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Bragulat Arara, Mª Rosa (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
The effects of a wide range of concentrations of 13 dyes on the colony diameters of nine fungal strains (including members of the Deuteromycetes and Zygomycetes) were evaluated. Auramine at a concentration of 50 ppm (50 ,ug/ml), methylene blue at a concentration of 500 ppm, gentian violet at a concentration of 5 ppm, and phenol red at a concentration of 50 ppm performed as well as the commonly used dyes dichloran at a concentration of 2 ppm and rose bengal at a concentration of 50 ppm in that they allowed adequate colony development of the Deuteromycetes strains tested and controlled rapidly spreading fungi.
1991
Applied and environmental microbiology, Vol. 57 Núm. 9 (Setembre 1991) , p. 2777-2780
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