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19 p, 492.8 KB Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris / Jordà Murria, Joel (Universitat Autònoma de Barcelona. Departament d’Enginyeria Química) ; Cueto Rojas, Hugo (Delft University of Technology. Department of Biotechnology) ; Carnicer Heras, Marc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Aljoscha Wahl (Delft University of Technology. Department of Biotechnology) ; Ferrer i Alegre, Pau (Universitat Autònoma de Barcelona. Departament d’Enginyeria Química) ; Albiol i Sala, Joan (Universitat Autonoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of the production of a Rhizopus oryzae lipase (Rol) on P. [...]
2014 - 10.3390/metabo4020281
Metabolites, Vol. 4 (2014) , p. 281-299  
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16 p, 760.8 KB Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary ¹³C flux analysis / Jordà Murria, Joel (Universitat Autònoma de Barcelona. Departament d’Enginyeria Química) ; Suárez, Camilo (Delft University of Technology. Department of Biotechnology) ; Carnicer Heras, Marc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; ten Pierick, Angela (Delft University of Technology. Department of Biotechnology) ; Heijnen, Joseph J. (Delft University of Technology. Department of Biotechnology) ; van Gulik, Walter (Delft University of Technology. Department of Biotechnology) ; Ferrer i Alegre, Pau (Universitat Autònoma de Barcelona. Departament d’Enginyeria Química) ; Albiol i Sala, Joan (Universitat Autonoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Aljoscha Wahl (Delft University of Technology. Department of Biotechnology)
Background: Several studies have shown that the utilization of mixed carbon feeds instead of methanol as sole carbon source is beneficial for protein production with the methylotrophic yeast Pichia pastoris. [...]
2013 - 10.1186/1752-0509-7-17
BMC systems biology, Vol. 7, Num. 17 (2013) , p. 1-16
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