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15 p, 1.2 MB |
Programmed death-ligand (PD-L1), epidermal growth factor (EGF), relaxin and metalloproteinase-3 (MMP3), potential biomarkers of malignancy in canine mammary neoplasia
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Galadima, Makchit ;
Teles, Mariana (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia) ;
Pastor Milán, Josep (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Hernandez-Losa, Javier (Hospital Universitari Vall d'Hebron) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Gene expression has been suggested as a putative tool for prognosis and diagnosis in canine mammary neoplasia (CMNs). In the present study, 58 formalin-fixed paraffin-embedded (FFPE) paraffined canine mammary neoplasias from 27 different bitches were included. [...]
2024 -  10.3390/ijms25021170
International journal of molecular sciences, Vol. 25 (2024) , p. 1170
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14 p, 1.3 MB |
Canine Mammary Neoplasia Induces Variations in the Peripheral Blood Levels of CD20, CD45RA, and CD99
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Galadima, Makchit (Sciomics GmbH) ;
Kotova, Iuliia (Sciomics GmbH) ;
Schmidt, Ronny (Sciomics GmbH) ;
Pastor Milán, Josep (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Schröder, Christoph (Sciomics GmbH) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
The idea of using tumour biomarkers as diagnostic tools is progressively increasing. Of these, serum biomarkers are of particular interest, as they can provide rapid results. In the present study, serum samples from 26 bitches diagnosed with mammary tumours, plus 4 healthy bitches, were obtained. [...]
2023 - 10.3390/ijms24119222
International journal of molecular sciences, Vol. 24 Núm. 11 (2023) , p. 9222-14
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14 p, 1.2 MB |
The effects of red LED light on pig sperm function rely upon mitochondrial electron chain activity rather than on a PKC-mediated mechanism
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Blanco Prieto, Olga (University of Bologna. Department of Veterinary Medicine Science) ;
Maside, Carolina (Universitat de Girona. Departament de Biologia) ;
Àlex Peña, None (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Ibáñez-Príncep, Júlia (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Bonet, Sergi (Universitat de Girona. Departament de Biologia) ;
Yeste Oliveras, Marc (Institució Catalana de Recerca i Estudis Avançats) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
While irradiation with red LED light has been reported to modulate sperm function in different mammalian species, the mechanisms underlying their response are poorly understood. This work sought to provide new insights into whether this effect relies on a direct action upon mitochondrial electron chain and/or on PKC-linked mechanisms such as those related to opsins. [...]
2022 - 10.3389/fcell.2022.930855
Frontiers in Cell and Developmental Biology, Vol. 10 (october 2022)
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12 p, 714.2 KB |
Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm Transcriptome by RNA-Seq
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Lian, Yu (Centre de Recerca en Agrigenòmica) ;
Gòdia, Marta (Centre de Recerca en Agrigenòmica) ;
Castelló Farré, Anna (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Balasch, Sam (Grup Gepork S.A. (Barcelona)) ;
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica) ;
Clop, Alex (Centre de Recerca en Agrigenòmica)
RNA-Seq data from human semen suggests that the study of the sperm transcriptome requires the previous elimination from the ejaculates of somatic cells carrying a larger load of RNA. Semen purification is also carried to study the sperm transcriptome in other species including swine and it is often done by density gradient centrifugation to obtain viable spermatozoa from fresh ejaculates or artificial insemination doses, thereby limiting the throughput and remoteness of the samples that can be processed in one study. [...]
2021 - 10.3389/fvets.2021.668158
Frontiers in Veterinary Science, Vol. 8 (July 2021) , art. 668158
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8 p, 1.7 MB |
Rat age-related benign prostate hyperplasia is concomitant with an increase in the secretion of low ramified α-glycosydic polysaccharides
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Trujillo-Rojas, Lina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Fernández Novell, Josep M.. (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular) ;
Blanco Prieto, Olga (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Martí-Garcia, Bernat (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals) ;
Rigau i Mas, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
This work analysed the expression of prostate polysaccharides in rats with age-related benign prostatic hyperplasia (BPH) for a better understanding of the possible relationship between prostate polysaccharides secretion and BPH onset. [...]
2022 - 10.1016/j.theriogenology.2022.06.011
Theriogenology, Vol. 189 (September 2022) , p. 150-157
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24 p, 3.2 MB |
Exogenous albumin is crucial for pig sperm to elicit in vitro capacitation whereas bicarbonate only modulates its efficiency
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Resende Chaves, Bruna (Universitat de Girona. Institut de Tecnologia Agroalimentària) ;
Pavaneli, Ana Paula (University of São Paulo. Department of Animal Reproduction) ;
Blanco Prieto, Olga (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Pinart, Elisabeth (Universitat de Girona. Departament de Biologia) ;
Bonet, Sergi (Universitat de Girona. Departament de Biologia) ;
Zangeronimo, Marcio Gilberto (Federal University of Lavras, Brazil. Department of Veterinary Medicine) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)
This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with different concentrations of bicarbonate (either 0 mM, 5 mM, 15 mM or 38 mM) and BSA (either 0 mg/mL or 5 mg/mL). [...]
2021 - 10.3390/biology10111105
Biology, Vol. 10 Núm. 11 (november 2021) , p. 1105
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191 p, 4.3 MB |
Photostimulation in boar sperm : mid-term effects, mechanism of action and "in-farm" applicability
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Blanco Prieto, Olga ;
Rodríguez Gil, Joan Enric, dir. ;
Rivera del Álamo, Ma. Montserrat, dir.
L'exposició a patrons de llum específics dels espermatozoides de diferents espècies animals, ja siguin mamífers o no mamífers, com conill, gall dindi, petits remugants, humans, tilàpia, gos i toro, millora els paràmetres de motilitat i augmenta l'activitat mitocondrial de la citocrom C oxidasa. [...]
La exposición a patrones de luz específicos de los espermatozoides de diferentes especies animales, ya sean mamíferos o no mamíferos, como conejo, pavo, pequeños rumiantes, humanos, tilapia, perro y toro; mejora los parámetros de movimiento y aumenta la actividad mitocondrial del citocromo C oxidasa. [...]
Exposition of sperm from separate species of mammalian and non-mammalian sperm such as rabbit, turkey, ram, human, tilapia, dog and bull to specific light patterns improves motion parameters as well as increases mitochondria cytochrome C oxidase activity. [...]
2020
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23 p, 3.1 MB |
Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms
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Blanco Prieto, Olga (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Catalán, Jaime (Universitat de Girona. Departament de Biologia) ;
Trujillo-Rojas, Lina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Peña, Alejandro (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Llavanera, Marc (Universitat de Girona. Departament de Biologia) ;
Bonet, Sergi (Universitat de Girona. Departament de Biologia) ;
Fernández Novell, Josep M.. (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular) ;
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia) ;
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. [...]
2020 - 10.3390/cells9122546
Cells, Vol. 9 Núm. 12 (26 2020)
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