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12 p, 5.0 MB Characterization of an anti-fetal AChR monoclonal antibody isolated from a myasthenia gravis patient / Saxena, Abhishek (Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University) ; Stevens, Jo (Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University) ; Cetin, Hakan (John Radcliffe Hospital (Oxford, Regne Unit)) ; Koneczny, Inga (Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University) ; Webster, Richard (John Radcliffe Hospital (Oxford, Regne Unit)) ; Lazaridis, Konstantinos (Hellenic Pasteur Institute) ; Tzartos, Socrates (Hellenic Pasteur Institute) ; Vrolix, Kathleen (Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University) ; Nogales, Gisela (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Machiels, Barbie (Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University) ; Molenaar, Peter C. (Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University) ; Damoiseaux, Jan (Central Diagnostic Laboratory, Maastricht University Medical Center) ; De Baets, Marc H. (Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University) ; Simon-Keller, Katja (Institute of Pathology, University Medical Centre Mannheim, University of Heidelberg) ; Marx, Alexander (Institute of Pathology, University Medical Centre Mannheim, University of Heidelberg) ; Vincent, Angela (John Radcliffe Hospital (Oxford, Regne Unit)) ; Losen, Mario (Maastricht University. Department of Psychiatry and Neuropsychology) ; Martinez-Martinez, Pilar (Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, Maastricht University) ; Universitat Autònoma de Barcelona
We report here the sequence and functional characterization of a recombinantly expressed.
autoantibody (mAb 131) previously isolated from a myasthenia gravis patient by immortalization of thymic B cells using Epstein-Barr virus and TLR9 activation. The antibody is characterized by a high degree of somatic mutations as well as a 6 amino acid insertion within the VHCDR2. [...]

2017 - 10.1038/s41598-017-14350-8
Scientific reports, Vol. 7 (october 2017) , p. 1-12  

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1 Damoiseaux, Jan
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