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11 p, 2.5 MB A bead-assisted flow cytometry method for the semi-quantitative analysis of Extracellular Vesicles / Suárez, Henar (Instituto de Investigación Hospital Universitario de la Princesa) ; Gámez-Valero, Ana (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Reyes, Raquel (Centro de Biología Molecular Severo Ochoa) ; López-Martín, Soraya (Instituto de Investigación Hospital Universitario de la Princesa) ; Rodríguez, María Josefa (Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas) ; Carrascosa, José L. (Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas) ; Cabañas, Carlos (Centro de Biología Molecular Severo Ochoa) ; Borràs i Serres, Francesc Enric (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Yáñez-Mó, María (Instituto de Investigación Hospital Universitario de la Princesa) ; Universitat Autònoma de Barcelona
Most experimental approaches commonly employed for the characterization and quantitation of EVs are time consuming, require of specialized instrumentation and often are rather inaccurate. To circumvent the caveats imposed by EV small size, we used general and specific membrane markers in bead assisted flow cytometry, to provide a semi-quantitative measure of EV content in a given sample. [...]
2017 - 10.1038/s41598-017-11249-2
Scientific reports, Vol. 7 (september 2017) , p. 1-11  

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