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6 p, 410.5 KB |
Side effects of chaperone gene co-expression in recombinant protein production
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Martínez Alonso, Mónica (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Garcia-Fruitos, Elena (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) ;
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Rinas, Ursula (Leibniz Universität Hannover) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Insufficient availability of molecular chaperones is observed as a major bottleneck for proper protein folding in recombinant protein production. Therefore, co-production of selected sets of cell chaperones along with foreign polypeptides is a common approach to increase the yield of properly folded, recombinant proteins in bacterial cell factories. [...]
2010 - 10.1186/1475-2859-9-64
Microbial cell factories, Vol. 9, Issue 64 (September 2010)
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35.
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36.
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37.
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15 p, 3.4 MB |
Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins
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Mamat, Uwe (Forschungszentrum Borstel) ;
Wilke, Kathleen (Forschungszentrum Borstel) ;
Bramhill, David (Research Corporation Technologies (Tucson)) ;
Schromm, Andra Beate (Forschungszentrum Borstel) ;
Lindner, Buko (Forschungszentrum Borstel) ;
Kohl, Thomas Andreas (Forschungszentrum Borstel) ;
Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Schaffer, Lana (Scripps Research Institute) ;
Head, Steven Robert (Scripps Research Institute) ;
Souvignier, Chad (Research Corporation Technologies (Tucson)) ;
Meredith, Timothy Charles (Pennsylvania State University. Department of Biochemistry and Molecular Biology) ;
Woodard, Ronald Wesley (Bramhill Biological Consulting)
Background: lipopolysaccharide (LPS), also referred to as endotoxin, is the major constituent of the outer leaflet of the outer membrane of virtually all Gram-negative bacteria. The lipid A moiety, which anchors the LPS molecule to the outer membrane, acts as a potent agonist for Toll-like receptor 4/myeloid differentiation factor 2-mediated pro-inflammatory activity in mammals and, thus, represents the endotoxic principle of LPS. [...]
2015 - 10.1186/s12934-015-0241-5
Microbial cell factories, Vol. 14, Issue 57 (2015)
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7 p, 774.5 KB |
Expanding the recombinant protein quality in Lactococcus lactis
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Cano-Garrido, Olivia (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Rueda, Fabián L. (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Sánchez-García, Laura (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Ruiz-Ávila, Luis (Spherium Biomed S.L.) ;
Bosser, Ramon (Spherium Biomed S.L.) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Garcia-Fruitos, Elena (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Background: Escherichia coli has been a main host for the production of recombinant proteins of biomedical interest, but conformational stress responses impose severe bottlenecks that impair the production of soluble, proteolytically stable versions of many protein species. [...]
2014 - 10.1186/s12934-014-0167-3
Microbial cell factories, Vol. 13 (2014) , p. 167
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16 p, 1.0 MB |
Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system : application on Pichia pastoris producing Rhizopus oryzae lipase
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Hemmerich, Johannes (m2p-labs GmbH) ;
Adelantado Vallvé, Núria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Barrigón de San Marcos, José Manuel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ponte Font, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Hörmann, Astrid (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Kensy, Frank (m2p-labs GmbH) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background: In Pichia pastoris bioprocess engineering, classic approaches for clone selection and bioprocess optimization at small/micro scale using the promoter of the alcohol oxidase 1 gene (PAOX1), induced by methanol, present low reproducibility leading to high time and resource consumption. [...]
2014 - 10.1186/1475-2859-13-36
Microbial cell factories, Vol. 13, No. 36 (March 2014) , p. 1-16
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8 p, 557.8 KB |
Microbial factories for recombinant pharmaceuticals
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Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Domingo-Espin, Joan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Most of the hosts used to produce the 151 recombinant pharmaceuticals so far approved for human use by the Food and Drug Administration (FDA) and/or by the European Medicines Agency (EMEA) are microbial cells, either bacteria or yeast. [...]
2009 - 10.1186/1475-2859-8-17
Microbial cell factories, Vol. 8, N. 17 (March 2009) , p. 1-8
2 documents
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10 p, 638.7 KB |
Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions
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Carnicer Heras, Marc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ten Pierick, Angela (Delft University of Technology. Department of Biotechnology (Delft, Països Baixos)) ;
van Dam, Jan (Delft University of Technology. Department of Biotechnology (Delft, Països Baixos)) ;
Heijnen, Joseph J. (Delft University of Technology. Department of Biotechnology (Delft, Països Baixos)) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
van Gulik, Walter (Delft University of Technology. Department of Biotechnology (Delft, Països Baixos)) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background: Environmental and intrinsic stress factors can result in the global alteration of yeast physiology, as evidenced by several transcriptional studies. Hypoxia has been shown to have a beneficial effect on the expression of recombinant proteins in Pichia pastoris growing on glucose. [...]
2012 - 10.1186/1475-2859-11-83
Microbial cell factories, Vol. 11, N. 83 (June 2012) , p. 1-10
4 documents
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