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16 p, 1.6 MB |
Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid
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Àvila Cabré, Sílvia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Pérez-Trujillo, Míriam (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Methanol is increasingly gaining attraction as renewable carbon source to produce specialty and commodity chemicals, as it can be generated from renewable sources such as carbon dioxide (CO2). In this context, native methylotrophs such as the yeast Komagataella phaffii (syn Pichia pastoris) are potentially attractive cell factories to produce a wide range of products from this highly reduced substrate. [...]
2023 -  10.1186/s12934-023-02241-9
Microbial Cell Factories, Vol. 22 (November 2023) , art. 237
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10 p, 1.9 MB |
A3DyDB : exploring structural aggregation propensities in the yeast proteome
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Garcia-Pardo, Javier (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ;
Badaczewska-Dawid, Aleksandra E. (Iowa State University) ;
Pintado-Grima, Carlos (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Iglesias, Valentin (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ;
Kuriata, Aleksander (University of Warsaw) ;
Kmiecik, Sebastian (University of Warsaw) ;
Ventura, Salvador (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Background: The budding yeast Saccharomyces cerevisiae (S. cerevisiae) is a well-established model system for studying protein aggregation due to the conservation of essential cellular structures and pathways found across eukaryotes. [...]
2023 - 10.1186/s12934-023-02182-3
Microbial cell factories, Vol. 22, Issue 1 (September 2023) , art. 186
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14 p, 1.9 MB |
Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography
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Carratalá, José Vicente (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Atienza-Garriga, Jan (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
López-Laguna, Hèctor (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Sanchez, Julieta M (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Background: Recombinant proteins cover a wide range of biomedical, biotechnological, and industrial needs. Although there are diverse available protocols for their purification from cell extracts or from culture media, many proteins of interest such as those containing cationic domains are difficult to purify, a fact that results in low yields of the final functional product. [...]
2023 - 10.1186/s12934-023-02081-7
Microbial cell factories, Vol. 22, Issue 1 (April 2023) , art. 81
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10 p, 1.3 MB |
Exploring the impact of the recombinant Escherichia coli strain on defensins antimicrobial activity : BL21 versus Origami strain
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López-Cano, Adrià (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal) ;
Martínez-Miguel, Marc (Networking Research Center On Bioengineering, Biomaterials and Nanomedicine) ;
Guasch, Judith (Max Planck Partner Group. Dynamic Biomimetics for Cancer Immunotherapy) ;
Ratera Bastardas, Imma (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) ;
Arís i Giralt, Anna (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal) ;
Garcia-Fruitos, Elena (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
The growing emergence of microorganisms resistant to antibiotics has prompted the development of alternative antimicrobial therapies. Among them, the antimicrobial peptides produced by innate immunity, which are also known as host defense peptides (HDPs), hold great potential. [...]
2022 - 10.1186/s12934-022-01803-7
Microbial cell factories, Vol. 21 (may 2022)
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16 p, 4.0 MB |
High throughput 13 C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
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Fina Romero, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Millard, Pierre (Université de Toulouse) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Heux, Stephanie (Université de Toulouse)
Production of 3-hydroxypropionic acid (3-HP) through the malonyl-CoA pathway has yielded promising results in Pichia pastoris (Komagataella phaffii), demonstrating the potential of this cell factory to produce this platform chemical and other acetyl-CoA-derived products using glycerol as a carbon source. [...]
2023 - 10.1186/s12934-023-02123-0
Microbial cell factories, Vol. 22 (June 2023) , art. 117
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17 p, 1.1 MB |
Recombinant vaccines in 2022 : a perspective from the cell factory
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de Pinho Favaro, Marianna T. (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Atienza-Garriga, Jan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Martínez-Torró, Carlos (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Parladé Molist, Eloi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
The last big outbreaks of Ebola fever in Africa, the thousands of avian influenza outbreaks across Europe, Asia, North America and Africa, the emergence of monkeypox virus in Europe and specially the COVID-19 pandemics have globally stressed the need for efficient, cost-effective vaccines against infectious diseases. [...]
2022 - 10.1186/s12934-022-01929-8
Microbial cell factories, Vol. 21 (October 2022) , art. 203
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13 p, 1.3 MB |
Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9
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Garrigos, Javier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Weninger, Astrid (Graz University of Technology) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Schmid, Christian (Graz University of Technology) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Rinnofner, Claudia (Austrian Centre of Industrial Biotechnology (ACIB)) ;
Glieder, Anton (Graz University of Technology) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Currently, the numerous and versatile applications in pharmaceutical and chemical industry make the recombinant production of cytochrome P450 enzymes (CYPs) of great biotechnological interest. Accelerating the drug development process by simple, quick and scalable access of human drug metabolites is key for efficient and targeted drug development in response to new and sometimes unexpected medical challenges and needs. [...]
2021 - 10.1186/s12934-021-01577-4
Microbial cell factories, Vol. 20 (April 2021) , art. 90
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12 p, 1.5 MB |
Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris
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Garrigos, Javier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Vuoristo, Kiira (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Nieto-Taype, Miguel Angel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Tähtiharju, Juha (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Uusitalo, Jaana (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Tukiainen, Pauliina (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Schmid, Christian (Bisy GmbH, Wuenschendorf 292, 8200 Hofstaetten/Raab, Austria) ;
Tolstorukov, Ilya (Keck Graduate Institute) ;
Madden, Knut (Biogrammatics Inc, 2794 Loker Ave, West, Suite 103, Carlsbad, CA 92010 USA) ;
Penttilä, Merja (VTT Technical Research Centre of Finland Ltd. Solutions for Natural Resources and Environment) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Glieder, Anton (Bisy GmbH, Wuenschendorf 292, 8200 Hofstaetten/Raab, Austria) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Pichia pastoris is a powerful and broadly used host for recombinant protein production (RPP), where past bioprocess performance has often been directed with the methanol regulated AOX1 promoter (P), and the constitutive GAP promoter (P). [...]
2021 - 10.1186/s12934-021-01564-9
Microbial cell factories, Vol. 20 (march 2021)
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