Depósito Digital de Documentos de la UAB Encontrados 46 registros  inicioanterior31 - 40siguiente  ir al registro: La búsqueda tardó 0.01 segundos. 
31.
6 p, 362.1 KB Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins / García Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; González Montalbán, Nuria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Morell Fernández, Montse (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Vera Barrón, Andrea (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ferraz Colomina, Rosa (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Arís i Giralt, Anna (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ventura, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; González Montalbán, Núria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ferraz Colomina, Rosa Maria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ventura Zamora, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Background: Many enzymes of industrial interest are not in the market since they are bio-produced as bacterial inclusion bodies, believed to be biologically inert aggregates of insoluble protein. Results: By using two structurally and functionally different model enzymes and two fluorescent proteins we show that physiological aggregation in bacteria might only result in a moderate loss of biological activity and that inclusion bodies can be used in reaction mixtures for efficient catalysis. [...]
2005 - 10.1186/1475-2859-4-27
Microbial cell factories, Vol. 4, N. 27 (September 2005) , p. 1-6  
32.
9 p, 405.6 KB The chaperone DnaK controls the fractioning of functional protein between soluble and insoluble cell fractions in inclusion body-forming cells / González Montalbán, Núria (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; García Fruitós, Elena (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Ventura, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular) ; Arís i Giralt, Anna (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ventura Zamora, Salvador (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Background: The molecular mechanics of inclusion body formation is still far from being completely understood, specially regarding the occurrence of properly folded, protein species that exhibit natural biological activities. [...]
2006 - 10.1186/1475-2859-5-26
Microbial cell factories, Vol. 5, N. 26 (August 2006) , p. 1-9  
33.
8 p, 333.6 KB Revisiting the Cellulosimicrobium cellulans yeast-lytic β-1,3-glucanases toolbox : A review / Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Cellulosimicrobium cellulans (also known with the synonyms Cellulomonas cellulans, Oerskovia xanthineolytica, and Arthrobacter luteus) is an actinomycete that excretes yeast cell wall lytic enzyme complexes containing endo-β-1,3-glucanases [EC 3. [...]
2006 - 10.1186/1475-2859-5-10
Microbial cell factories, Vol. 5, N. 10 (March 2006) , p. 1-8  
34.
20 p, 435.6 KB Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters : A review / Cos Busquets, Josep Oriol (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Ramon Real, Ramon (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for heterologous protein production. The use of different phenotypes under PAOX promoter, other alternative promoters, culture medium, and operational strategies with the objective to maximize either yield or productivity of the heterologous protein, but also to obtain a repetitive product batch to batch to get a robust process for the final industrial application have been reported. [...]
2006 - 10.1186/1475-2859-5-17
Microbial cell factories, Vol. 5, N. 17 (April 2006) , p. 1-20  
35.
7 p, 318.2 KB Insertional protein engineering for analytical molecular sensing / Ferraz Colomina, Rosa Maria (Universitat Politècnica de Catalunya. Departament de Matemática Aplicada IV) ; Vera Barrón, Andrea (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Arís i Giralt, Anna (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
The quantitative detection of low analyte concentrations in complex samples is becoming an urgent need in biomedical, food and environmental fields. Biosensors, being hybrid devices composed by a biological receptor and a signal transducer, represent valuable alternatives to non biological analytical instruments because of the high specificity of the biomolecular recognition. [...]
2006 - 10.1186/1475-2859-5-15
Microbial cell factories, Vol. 5, N. 15 (April 2006) , p. 1-7  
36.
11 p, 1.1 MB Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction / Resina Rodríguez, David (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Bollók, Mónika (University of Oulu. Department of Process and Environmental Engineering and Biocenter (Oulu, Finlàndia)) ; Khatri, Narendar K. (University of Oulu. Department of Process and Environmental Engineering and Biocenter (Oulu, Finlàndia)) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ; Neubauer, Peter (University of Oulu. Department of Process and Environmental Engineering and Biocenter (Oulu, Finlàndia)) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Background: The analysis of transcriptional levels of the genes involved in protein synthesis and secretion is a key factor to understand the host organism's responses to recombinant protein production, as well as their interaction with the cultivation conditions. [...]
2007 - 10.1186/1475-2859-6-21
Microbial cell factories, Vol. 6, N. 21 (July 2007) , p. 1-11  
37.
2 p, 193.8 KB Systems biology and biological systems diversity for the engineering of microbial cell factories : editorial / Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
2007 - 10.1186/1475-2859-6-35
Microbial cell factories, Vol. 6, N. 35 (November 2007) , p. 1-2  
38.
7 p, 441.8 KB Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris / Surribas i Casalprim, Anna (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Resina Rodríguez, David (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background: Together with the development of optical sensors, fluorometry is becoming an increasingly attractive tool for the monitoring of cultivation processes. In this context, the green fluorescence protein (GFP) has been proposed as a molecular reporter when fused to target proteins to study their subcellular localization or secretion behaviour. [...]
2007 - 10.1186/1475-2859-6-15
Microbial cell factories, Vol. 6, N. 15 (May 2007) , p. 1-7  
39.
18 p, 567.4 KB Protein folding and conformational stress in microbial cells producing recombinant proteins : a host comparative overview / Gasser, Brigitte (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Saloheimo, Markku (VTT Technical Research Centre (Espoo, Finlàndia)) ; Rinas, Ursula (Helmholtz Center for Infection Research (Braunschweig, Alemanya)) ; Dragosits, Martin (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Rodríguez-Carmona, Escarlata (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Baumann, Kristin (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Giuliani, Maria (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia)) ; Parrilli, Ermenegilda (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia)) ; Branduardi, Paola (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Milà, Itàlia)) ; Lang, Christine (Technical University Berlin. Institute for Microbiology and Genetics) ; Porro, Danilo (University of Milano-Bicocca. Department of Biotechnology and Bioscience (Milà, Itàlia)) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Tutino, Maria Luisa (University of Naples Federico II. School of Biotechnological Sciences (Nàpols, Itàlia)) ; Mattanovich, Diethard (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. [...]
2008 - 10.1186/1475-2859-7-11
Microbial cell factories, Vol. 7, N. 11 (April 2008) , p. 1-18  
40.
14 p, 879.6 KB Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels / Carnicer Heras, Marc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Baumann, Kristin (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Töplitz, Isabelle (University of Applied Sciences. School of Bioengineering (Viena, Àustria)) ; Sánchez-Ferrando, Francisco (Universitat Autònoma de Barcelona. Departament de Química) ; Mattanovich, Diethard (University of Natural Resources and Life Sciences. Department of Biotechnology (Viena, Àustria)) ; Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química) ; Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Background: Analysis of the cell operation at the metabolic level requires collecting data of different types and to determine their confidence level. In addition, the acquired information has to be combined in order to obtain a consistent operational view. [...]
2009 - 10.1186/1475-2859-8-65
Microbial cell factories, Vol. 8, N. 65 (December 2009) , p. 1-14
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Depósito Digital de Documentos de la UAB : Encontrados 46 registros   inicioanterior31 - 40siguiente  ir al registro:
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