|
1.
|
|
13 p, 2.6 MB |
Synergic kinetic and physiological control to improve the efficiency of Komagataella phaffii recombinant protein production bioprocesses
/
Sales-Vallverdú, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gasset Franch, Arnau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Requena-Moreno, Guillermo (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The yeast Komagataella phaffii (Pichia pastoris) is currently considered a versatile and highly efficient host for recombinant protein production (RPP). Interestingly, the regulated application of specific stress factors as part of bioprocess engineering strategies has proven potential for increasing the production of recombinant products. [...]
2024 -  10.1111/1751-7915.14411
Microbial biotechnology, Vol. 17, Issue 2 (February 2024) , art. e14411
|
|
|
2.
|
|
11 p, 2.2 MB |
Tuning plasmid DNA amounts for cost-effective transfections of mammalian cells : when less is more
/
Carreño, Aida (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Guerrero-Yagüe, Rubén (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Casal, Enriqueta (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Mendoza, Rosa (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ;
Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Transient gene expression (TGE) in mammalian cells is a well-known approach to the fast expression of recombinant proteins. The human cell line HEK (human embryonic kidney) 293F is widely used in this field, due to its adaptability to grow in suspension to high cell densities in serum-free media, amenability to transfection, and production of recombinant proteins in satisfactory quantities for functional and structural analysis. [...]
2024 - 10.1007/s00253-024-13003-x
Applied Microbiology and Biotechnology, Vol. 108 (January 2024) , art. 98
|
|
|
3.
|
|
16 p, 1.4 MB |
CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
/
Oliveira, Hugo (LABBELS -Associate Laboratory) ;
Santos, Sílvio (LABBELS -Associate Laboratory) ;
Pires, Diana P. (LABBELS -Associate Laboratory) ;
Boeckaerts, Dimitri (Ghent University) ;
Pinto, Graça (LABBELS -Associate Laboratory) ;
Domingues, Rita (LABBELS -Associate Laboratory) ;
Otero Carrera, Jennifer (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Briers, Yves (Ghent University) ;
Lavigne, Rob (KU Leuven) ;
Schmelcher, Mathias (ETH Zurich) ;
Dötsch, Andreas (Max Rubner-Institute) ;
Azeredo, Joana (LABBELS -Associate Laboratory)
Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. [...]
2023 - 10.1007/s00253-023-12547-8
Applied Microbiology and Biotechnology, Vol. 107 (May 2023) , p. 3621-3636
|
|
|
4.
|
|
|
5.
|
|
|
6.
|
|
8 p, 1.4 MB |
Improving the assessment of ecosystem and wildlife health : microbiome as an early indicator
/
Puig Ribas, Maria (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
García-Ulloa, Manuel (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ;
Espunyes, Johan (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ;
Cabezón Ponsoda, Óscar (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Human activities are causing dramatic declines in ecosystem health, compromising the functioning of the life-support system, economic activity, and animal and human health. In this context, monitoring the health of ecosystems and wildlife populations is crucial for determining ecological dynamics and assessing management interventions. [...]
2023 - 10.1016/j.copbio.2023.102923
Current opinion in biotechnology, Vol. 81 (2023) , art. 102923
|
|
|
7.
|
|
8 p, 1.4 MB |
Preservation of critical quality attributes of mesenchymal stromal cells in 3D bioprinted structures by using natural hydrogel scaffolds
/
Martorell Cedres, Lluis (Banc de Sang i Teixits. Edifici Dr. Frederic Duran i Jordà) ;
López-Fernández, Alba (Hospital Universitari Vall d'Hebron. Institut de Recerca) ;
García-Lizarribar, Andrea (Institut de Bioenginyeria de Catalunya) ;
Sabata, Roger (R&D Human Health. Bioibérica (Barcelona)) ;
Galvez-Martin, Patricia (R&D Human Health. Bioibérica (Barcelona)) ;
Samitier, Josep (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina (Barcelona)) ;
Vives Armengol, Joaquim (Universitat Autònoma de Barcelona. Departament de Medicina)
Three dimensional (3D) bioprinting is an emerging technology that enables complex spatial modeling of cell-based tissue engineering products, whose therapeutic potential in regenerative medicine is enormous. [...]
2023 - 10.1002/bit.28381
Biotechnology and Bioengineering, Vol. 120 Núm. 9 (september 2023) , p. 2717-2724
|
|
|
8.
|
|
9 p, 1.9 MB |
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens
/
Campbell, Amy M. (Centre for Environment, Fisheries and Aquaculture Science) ;
Hauton, Chris (University of Southampton. School of Ocean and Earth Science) ;
Baker-Austin, Craig (Centre for Environment, Fisheries and Aquaculture Science) ;
van Aerle, Ronny (Centre for Environment, Fisheries and Aquaculture Science) ;
Martinez-Urtaza, Jaime (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
It is critical to gain insight into how climate change impacts evolutionary responses within climate-sensitive pathogen populations, such as increased resilience, opportunistic responses and the emergence of dominant variants from highly variable genomic backgrounds and subsequent global dispersal. [...]
2023 - 10.1016/j.copbio.2023.102898
Current opinion in biotechnology, Vol. 80 (April 2023) , art. 102898
|
|
|
9.
|
|
13 p, 1.2 MB |
Downstream process design for Gag HIV-1 based virus-like particles
/
Lorenzo, Elianet (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Miranda, Laia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Gòdia, Francesc (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cervera Gracia, Laura (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Virus-like particles-based vaccines have been gaining interest in recent years. The manufacturing of these particles includes their production by cell culture followed by their purification to meet the requirements of its final use. [...]
2023 - 10.1002/bit.28419
Biotechnology and Bioengineering, Vol. 120, Issue 9 (September 2023) , p. 2672-2684
|
|
|
10.
|
|
16 p, 1.9 MB |
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P promoter
/
Bernat Camps, Núria (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ebner, Katharina (Bisy GmbH) ;
Schusterbauer, Veronika (Bisy GmbH) ;
Fischer, Jasmin Elgin (Bisy GmbH) ;
Nieto-Taype, Miguel Angel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Glieder, Anton (Bisy GmbH) ;
Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of Komagataella phaffii (Pichia pastoris) can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-product from the biodiesel industry, while producing high- and low-added value products. [...]
2023 - 10.3389/fbioe.2023.1130583
Frontiers in Bioengineering and Biotechnology, Vol. 11 (March 2023) , art. 1130583
|
|
visitante ::
identificación
alerta personal vía correo electrónico o subscríbase al 
canal RSS.
