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1.
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16 p, 1.6 MB |
Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid
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Àvila Cabré, Sílvia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Pérez-Trujillo, Míriam (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Methanol is increasingly gaining attraction as renewable carbon source to produce specialty and commodity chemicals, as it can be generated from renewable sources such as carbon dioxide (CO2). In this context, native methylotrophs such as the yeast Komagataella phaffii (syn Pichia pastoris) are potentially attractive cell factories to produce a wide range of products from this highly reduced substrate. [...]
2023 -  10.1186/s12934-023-02241-9
Microbial Cell Factories, Vol. 22 (November 2023) , art. 237
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2.
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14 p, 7.1 MB |
A long-lasting porcine model of ARDS caused by pneumonia and ventilator-induced lung injury
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Barbeta, Enric (Hospital Clínic i Provincial de Barcelona) ;
Arrieta, Marta (Universitat de Barcelona) ;
Motos, Ana (Universitat de Barcelona) ;
Bobi, Joaquim (Hospital Clínic i Provincial de Barcelona) ;
Yang, Hua (Beijing Chao-Yang Hospital) ;
Yang, Minlan (Beijing Chao-Yang Hospital) ;
Tanzella, Giacomo (San Martino Policlinico Hospital) ;
Di Ginnatale, Pierluigi (SS. Annunziata Hospital) ;
Nogas, Stefano (San Martino Policlinico Hospital) ;
Vargas, Carmen Rosa (Universitat de Barcelona) ;
Cabrera, Roberto (Institut d'Investigacions Biomèdiques August Pi i Sunyer) ;
Battaglini, Denise (San Martino Policlinico Hospital) ;
Meli, Andrea (Fondazione IRCCS Ca'Granda Ospedale Maggiore Policlinico) ;
Kiarostami, Kasra (Universitat de Barcelona) ;
Vázquez, Nil (Universitat de Barcelona) ;
Fernández-Barat, Laia (Universitat de Barcelona) ;
Rigol, Montserrat (Hospital Clínic i Provincial de Barcelona) ;
Mellado-Artigas, Ricard (Hospital Clínic i Provincial de Barcelona) ;
Frigola, Gerard (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT)) ;
Camprubí-Rimblas, Marta (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT)) ;
Ferrer, Pau (Universitat Pompeu Fabra) ;
Martinez, Daniel (Hospital Clínic i Provincial de Barcelona) ;
Artigas Raventós, Antoni (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT)) ;
Ferrando, Carlos (Hospital Clínic i Provincial de Barcelona) ;
Ferrer, Miquel (Hospital Clínic i Provincial de Barcelona) ;
Torres, Antoni (Hospital Clínic i Provincial de Barcelona) ;
Universitat Autònoma de Barcelona
Animal models of acute respiratory distress syndrome (ARDS) do not completely resemble human ARDS, struggling translational research. We aimed to characterize a porcine model of ARDS induced by pneumonia-the most common risk factor in humans-and analyze the additional effect of ventilator-induced lung injury (VILI). [...]
2023 - 10.1186/s13054-023-04512-8
Critical Care, Vol. 27 (june 2023)
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3.
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30 p, 997.5 KB |
Glycerol metabolism of Pichia pastoris (Komagataella spp.) characterised by 13 C-based metabolic flux analysis
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Tomàs Gamisans, Màrius (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ødum, Anders Sebastian Rosenkrans (Technical University of Denmark. Department of Biotechnology and Biomedicine) ;
Workman, Mhairi (Novo Nordisk) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Metabolic flux analysis based on 13 C-derived constraints has proved to be a powerful method for quantitative physiological characterisation of one of the most extensively used microbial cell factory platforms, Pichia pastoris (syn. [...]
2019 - 10.1016/j.nbt.2019.01.005
New Biotechnology, Vol. 50 (May 2019) , p. 52-59
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4.
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34 p, 614.1 KB |
Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?
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Garcia-Ortega, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Cámara, Elena (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Montesinos Seguí, José Luis (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The increasing demand for recombinant proteins for a wide range of applications, from biopharmaceutical protein complexes to industrial enzymes, is leading to important growth in this market. Among the different efficient host organism alternatives commonly used for protein production, the yeast Pichia pastoris (Komagataella phaffii) is currently considered to be one of the most effective and versatile expression platforms. [...]
2019 - 10.1016/j.nbt.2019.06.002
New Biotechnology, Vol. 53 (November 2019) , p. 24-34
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5.
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42 p, 1.2 MB |
Rheological and structural characterisation of whey protein acid gels co-structured with chia (Salvia hispanica L.) or flax seed (Linum usitatissimum L.) mucilage
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Soukoulis, Christos (Luxembourg Institute of Science and Technology. Environmental Research and Innovation) ;
Cambier, Sébastien (Luxembourg Institute of Science and Technology. Environmental Research and Innovation) ;
Serchi, Tommaso (Luxembourg Institute of Science and Technology. Environmental Research and Innovation) ;
Tsevdou, Maria (National Technical University of Athens) ;
Gaiani, Claire (Université de Lorraine) ;
Ferrer, Pau (Luxembourg Institute of Science and Technology. Environmental Research and Innovation) ;
Taoukis, Petros S. (National Technical University of Athens) ;
Hoffmann, Lucien (Luxembourg Institute of Science and Technology. Environmental Research and Innovation)
The effects of different plant seed mucilage (PSM) extracts, namely chia seed (CSM) and flaxseed (FSM), on the kinetics of δ-glucono-lactone induced acidification and gelation phenomena of whey proteins (5% w/w WPI) were investigated. [...]
2019 - 10.1016/j.foodhyd.2018.11.002
Food Hydrocolloids, Vol. 89 (April 2022) , p. 542-553
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6.
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16 p, 4.0 MB |
High throughput 13 C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
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Fina Romero, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Millard, Pierre (Université de Toulouse) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Heux, Stephanie (Université de Toulouse)
Production of 3-hydroxypropionic acid (3-HP) through the malonyl-CoA pathway has yielded promising results in Pichia pastoris (Komagataella phaffii), demonstrating the potential of this cell factory to produce this platform chemical and other acetyl-CoA-derived products using glycerol as a carbon source. [...]
2023 - 10.1186/s12934-023-02123-0
Microbial cell factories, Vol. 22 (June 2023) , art. 117
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7.
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28 p, 460.0 KB |
Enzymatic production of biodiesel : Strategies to overcome methanol inactivation
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Lotti, Marina (Università degli Studi Milano-Bicocca. Dipartimento di Biotecnologie e Bioscienze) ;
Pleiss, Jürgen (University of Stuttgart. Institute of Biochemistry and Technical Biochemistry) ;
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Lipase-catalyzed transesterification of triglycerides and alcohols to obtain biodiesel is an environmentally friendly and sustainable route for fuels production since, besides proceeding in mild reaction conditions, it allows for the use of low-cost feedstocks that contain water and free fatty acids, for example non-edible oils and waste oils. [...]
2018 - 10.1002/biot.201700155
Biotechnology Journal, Vol. 13, Issue 5 (May 2018) , art. 1700155
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8.
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14 p, 1.6 MB |
Novel CaLB-like Lipase Found Using ProspectBIO, a Software for Genome-Based Bioprospection
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Brêda, Gabriela C. (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Faria, Priscila E. (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Rodrigues, Yuri S. (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Pinheiro, Priscila B. (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Nucci, Maria Clara R. (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Freire, Denise M. G. (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Volcan Almeida, Rodrigo (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica) ;
Mesquita, Rafael D. (Universidade Federal do Rio de Janeiro. Departamento de Bioquímica)
Enzymes have been highly demanded in diverse applications such as in the food, pharmaceutical, and industrial fuel sectors. Thus, in silico bioprospecting emerges as an efficient strategy for discovering new enzyme candidates. [...]
2023 - 10.3390/biotech12010006
BioTech, Vol. 12, Issue 1 (January 2023) , art. 6
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9.
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40 p, 1.6 MB |
Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter
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Cámara, Elena (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Monforte, Sergi (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Albiol i Sala, Joan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental) ;
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
The methanol-regulated alcohol oxidase promoter (PAOX1) of Pichia pastoris (syn. Komagataella spp. ) is one of the strongest promoters for heterologous gene expression. Although increasing the gene dosage is a common strategy to improve recombinant protein productivities, P. [...]
2019 - 10.1002/bit.26947
Biotechnology and Bioengineering, Vol. 116, Issue 7 (July 2019) , p. 1710-1720
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10.
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16 p, 2.4 MB |
Carbohydrate hydrolytic potential and redundancy of an anaerobic digestion microbiome exposed to acidosis, as uncovered by metagenomics
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Bertucci, Marie (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Calusinska, Magdalena (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Goux, Xavier (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Rouland-Lefèvre, Corinne (Sorbonne Universités. Institut de Recherche Développement. Institut d'Ecologie et des Sciences de l'Environnement) ;
Untereiner, Boris (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Ferrer, Pau (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department) ;
Gerin, Patrick A. (Université Catholique de Louvain. Laboratory of Bioengineering, Earth) ;
Delfosse, Philippe (Luxembourg Institute of Science and Technology. Environmental Research and Innovation Department)
Increased hydrolysis of easily digestible biomass may lead to acidosis of anaerobic reactors and decreased methane production. Previously, it was shown that the structure of microbial communities changed during acidosis; however, once the conditions are back to optimal, biogas (initially CO2) production quickly restarts. [...]
2019 - 10.1128/AEM.00895-19
Applied and Environmental Microbiology, Vol. 85, Issue 15 (August 2019) , p. e00895-19
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