Patterned anchorage to the apical extracellular matrix defines tissue shape in the developing appendages of Drosophila
Ray, Robert P. (University of Sussex. School of Life Sciences)
Matamoro-Vidal, Alexis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Ribeiro, Paulo S. (The Francis Crick Institute)
Tapon, Nic (The Francis Crick Institute)
Houle, David (Florida State University. Department of Biological Science)
Salazar Ciudad, Isaac (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Thompson, Barry J. (The Francis Crick Institute)

Date:	2015
Abstract:	How tissues acquire their characteristic shape is a fundamental unresolved question in biology. While genes have been characterized that control local mechanical forces to elongate epithelial tissues, genes controlling global forces in epithelia have yet to be identified. Here, we describe a genetic pathway that shapes appendages in Drosophila by defining the pattern of global tensile forces in the tissue. In the appendages, shape arises from tension generated by cell constriction and localized anchorage of the epithelium to the cuticle via the apical extracellular-matrix protein Dumpy (Dp). Altering Dp expression in the developing wing results in predictable changes in wing shape that can be simulated by a computational model that incorporates only tissue contraction and localized anchorage. Three other wing shape genes, narrow, tapered, and lanceolate, encode components of a pathway that modulates Dp distribution in the wing to refine the global force pattern and thus wing shape. Regulation of global tensile forces in epithelia is one mechanism of determining tissue shape. Ray and Matamoro-Vidal et al. show that tissue contraction, in combination with localized anchorage to the cuticle by the apical extracellular matrix protein Dumpy, gives rise to anisotropic tensions that shape the appendages in the Drosophila pupa.
Note:	Ajuts: This work was supported by a Wellcome Trust Investigator Award to B.J.T., a Genetics Society Summer Studentship and research grants from the BBSRC (BB/C508050/1) and MRC (G0500916) to R.P.R., an NIH grant (5R01GM094424-04) to D.H., and a Finnish Academy grant (WBS 1250271) to I.S.-C.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Developmental cell, Vol. 34, issue 3 (Aug. 2015) , p. 310-322, ISSN 1878-1551

DOI: 10.1016/j.devcel.2015.06.019
PMID: 26190146

14 p, 4.8 MB

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