Identification and characterization of inorganic‑phosphate‑solubilizing bacteria from agricultural fields with a rapid isolation method
Zheng, Bang-Xiao (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Ibrahim, Muhammad (Chinese Academy of Sciences. Key Laboratory of Urban Environment and Health)
Zhang, Ding-Peng (un Yat-sen University. State Key Laboratory of Biocontrol)
Bi, Qing-Fang (Chinese Academy of Sciences. Key Laboratory of Urban Environment and Health)
Li, Hong-Zhe (Chinese Academy of Sciences. Key Laboratory of Urban Environment and Health)
Zhou, Guo-Wei (Chinese Academy of Sciences. State Key Laboratory of Urban and Regional Ecology)
Ding, Kai (Chinese Academy of Sciences. State Key Laboratory of Urban and Regional Ecology)
Peñuelas, Josep (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Zhu, Yong-Guan (Chinese Academy of Sciences. Key Laboratory of Urban Environment and Health)
Yang, Xiao-Ru (Chinese Academy of Sciences. Key Laboratory of Urban Environment and Health)

Fecha:	2018
Resumen:	The ability to solubilize fixed inorganic phosphorus (P) for plant growth is important for increasing crop yield. More P can be released by inoculating soil with inorganic-phosphate-solubilizing bacteria (iPSBs). We used 96-well microplates instead of traditional 200-mm petri dishes to rapidly screen iPSB strains for their solubilizing ability. We simultaneously obtained 76 iPSB isolates from 576 wells containing two agricultural soils. This method conveniently identified positive iPSB strains and effectively prevented fungal cross-contamination. Maximum-likelihood phylogenetic trees of the isolated strains showed that Bacillus megaterium was the most dominant iPSB, and strains Y99, Y95, Y924 and Y1412 were selected as representatives for the analysis of P solubilization. Succinic acid was the main organic acid of B. megateriumfor releasing P. It was strongly correlated with the increase in soluble P concentration during 168 h of incubation of these four strains. pH was negatively exponentially correlated with the amount of soluble P in the medium, and the amount of succinic acid was strongly linearly correlated with the amount of P released (P < 0. 001), suggesting that organic acid may mobilize microbial P. Our study provides an efficient and effective method for identifying and analyzing the growth of iPSB strains able to solubilize inorganic P and gives a better understanding of the mechanism of P solubilization.
Ayudas:	European Commission 610028
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Phosphorus ; Inorganic phosphate solubilizing bacteria ; Isolation ; Characterization
Publicado en:	AMB express, Vol. 8, issue 47 (March 2018) , ISSN 2191-0855

PMID: 29589217

12 p, 1.7 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CREAF (Centre de Recerca Ecològica i d'Aplicacions Forestals) > Imbalance-P
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