Effects of liposomal formulation of citicoline in experimental diabetes-induced retinal neurodegeneration
Bogdanov, Patricia (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Sampedro, Joel (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Solà-Adell, Cristina (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Simó-Servat, Olga (Universitat Autònoma de Barcelona. Departament de Medicina)
Russo, Carla (R&D Department. Omikron Italia Srl)
Varela-Sende, Luisa (Clinical Research Department. OPKO Health Spain S.L)
Simó Canonge, Rafael (Universitat Autònoma de Barcelona. Departament de Medicina)
Hernández, Cristina (Universitat Autònoma de Barcelona. Departament de Medicina)

Fecha:	2018
Resumen:	Diabetic retinopathy (DR) has been classically considered a microcirculatory disease of the retina. However, there is growing evidence to suggest that retinal neurodegeneration is also an early event in the pathogenesis of DR. Citicoline has been successfully used as a neuroprotective agent in the treatment of glaucoma but their effects on DR remain to be elucidated. On this basis, the main aim of the present study was to evaluate the effect of topical administration of citicoline in liposomal formulation on retinal neurodegeneration in db/db mouse and to investigate the underlying mechanisms of action. The treatment (citicoline or vehicle) was topically administered twice daily for 15 days. Retinal analyses were performed in vivo by electroretinography and ex vivo by using Western blot and immunofluorescence measurements. We found that the liposomal formulation of citicoline prevented glial activation and neural apoptosis in the diabetic retina. The main mechanism implicated in these beneficial effects were the inhibition of the downregulation of synaptophysin and its anti-inflammatory properties by means of preventing the upregulation of NF-κB and TNF-α (Tumor Necrosis Factor α) induced by diabetes. Overall, these results suggest that topical administration of citicoline in liposomal formulation could be considered as a new strategy for treating the early stages of DR.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Citicoline ; Diabetic retinopathy ; Retinal neurodegeneration ; Db/db mouse
Publicado en:	International journal of molecular sciences, Vol. 19 Núm. 8 (20 2018) , p. 2458, ISSN 1422-0067

DOI: 10.3390/ijms19082458
PMID: 30127248

15 p, 4.2 MB

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