Versatile Graphene-Based Platform for Robust Nanobiohybrid Interfaces
Bueno, Rebeca (Institute of Materials Science of Madrid)
Marciello, Marzia (Universidad Complutense de Madrid)
Moreno, Miguel (Centro de Astrobiología (Madrid))
Sánchez Sánchez, Carlos (Institute of Materials Science of Madrid)
Martínez, José I. (Institute of Materials Science of Madrid)
Martínez, Lidia (Institute of Materials Science of Madrid)
Prats Alfonso, Elisabet (Institut de Microelectrònica de Barcelona)
Guimerà Brunet, Anton (Institut de Microelectrònica de Barcelona)
Garrido, Jose (Institut Català de Nanociència i Nanotecnologia)
Villa, Rosa (Institut de Microelectrònica de Barcelona)
Mompean, Federico (Institute of Materials Science of Madrid)
Garcia-Hernandez, Mar (Institute of Materials Science of Madrid)
Huttel, Yves (Institute of Materials Science of Madrid)
Morales, María del Puerto (Institute of Materials Science of Madrid)
Briones Llorente, Carlos (Centro de Astrobiología (Madrid))
López, María F. (Institute of Materials Science of Madrid)
Ellis, Gary J. (Institute of Polymer Science and Technology)
Vázquez, Luis (Institute of Materials Science of Madrid)
Martín Gago, José A. (Institute of Materials Science of Madrid)

Date:	2019
Abstract:	Technologically useful and robust graphene-based interfaces for devices require the introduction of highly selective, stable, and covalently bonded functionalities on the graphene surface, whilst essentially retaining the electronic properties of the pristine layer. This work demonstrates that highly controlled, ultrahigh vacuum covalent chemical functionalization of graphene sheets with a thiol-terminated molecule provides a robust and tunable platform for the development of hybrid nanostructures in different environments. We employ this facile strategy to covalently couple two representative systems of broad interest: metal nanoparticles, via S-metal bonds, and thiol-modified DNA aptamers, via disulfide bridges. Both systems, which have been characterized by a multitechnique approach, remain firmly anchored to the graphene surface even after several washing cycles. Atomic force microscopy images demonstrate that the conjugated aptamer retains the functionality required to recognize a target protein. This methodology opens a new route to the integration of high-quality graphene layers into diverse technological platforms, including plasmonics, optoelectronics, or biosensing. With respect to the latter, the viability of a thiol-functionalized chemical vapor deposition graphene-based solution-gated field-effect transistor array was assessed.
Grants:	European Commission 696656 European Commission 785219 European Commission 610256 Ministerio de Economía y Competitividad MAT2014-54231-C4-1-P Ministerio de Economía y Competitividad MAT2014-54231-C4-4-P Ministerio de Economía y Competitividad MAT2017-85089-C2-1-R Ministerio de Economía y Competitividad MAT2014-59772-C2-2-P Ministerio de Economía y Competitividad BIO2016-79618-R Ministerio de Economía y Competitividad IJCI-2014-19291
Note:	This is an open access article published under an ACS AuthorChoice License. See Standard ACS AuthorChoice/Editors' Choice Usage Agreement - https://pubs.acs.org/page/policy/authorchoice_termsofuse.html
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	ACS omega, Vol. 4, Issue 2 (February 2019) , p. 3287-3297, ISSN 2470-1343

DOI: 10.1021/acsomega.8b03152
PMID: 31008418

11 p, 5.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles