Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates
Yeste Oliveras, Marc (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Codony, Francesc (GenIUL)
Estrada, Efrén (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Lleonart, Miquel (GenIUL)
Balasch, Sam (Servicios Genéticos Porcinos, S.L)
Peña, Alejandro (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Bonet, Sergi (Universitat de Girona. Biotechnology of Animal and Human Reproduction (TechnoSperm))
Rodríguez-Gil, Joan E. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Date:	2016
Abstract:	The present study evaluated the effects of exposing liquid-stored boar semen to different red light LED regimens on sperm quality and reproductive performance. Of all of the tested photo-stimulation procedures, the best pattern consisted of 10min light, 10min rest and 10min of further light (10-10-10 pattern). This pattern induced an intense and transient increase in the majority of motility parameters, without modifying sperm viability and acrosome integrity. While incubating non-photo-stimulated sperm at 37°C for 90min decreased all sperm quality parameters, this reduction was prevented when the previously-described light procedure was applied. This effect was concomitant with an increase in the percentage of sperm with high mitochondrial membrane potential. When sperm were subjected to 'in vitro' capacitation, photo-stimulation also increased the percentage of sperm with capacitation-like changes in membrane structure. On the other hand, treating commercial semen doses intended for artificial insemination with the 10-10-10 photo-stimulation pattern significantly increased farrowing rates and the number of both total and live-born piglets for parturition. Therefore, our results indicate that a precise photo-stimulation procedure is able to increase the fertilising ability of boar sperm via a mechanism that could be related to mitochondrial function.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Esperma ; Porcs senglars
Published in:	Scientific reports, Vol. 6 (March 2016) , art. 22569, ISSN 2045-2322

DOI: 10.1038/srep22569
PMID: 26931070

13 p, 933.8 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles