Advanced Evanescent-Wave Optical Biosensors for the Detection of Nucleic Acids : An Analytic Perspective
Huertas, César S. (Royal Melbourne Institute of Technology University)
Calvo Lozano, Olalla (Institut Català de Nanociència i Nanotecnologia)
Mitchell, Arnan (Royal Melbourne Institute of Technology University)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Fecha:	2019
Resumen:	Evanescent-wave optical biosensors have become an attractive alternative for the screening of nucleic acids in the clinical context. They possess highly sensitive transducers able to perform detection of a wide range of nucleic acid-based biomarkers without the need of any label or marker. These optical biosensor platforms are very versatile, allowing the incorporation of an almost limitless range of biorecognition probes precisely and robustly adhered to the sensor surface by covalent surface chemistry approaches. In addition, their application can be further enhanced by their combination with different processes, thanks to their integration with complex and automated microfluidic systems, facilitating the development of multiplexed and user-friendly platforms. The objective of this work is to provide a comprehensive synopsis of cutting-edge analytical strategies based on these label-free optical biosensors able to deal with the drawbacks related to DNA and RNA detection, from single point mutations assays and epigenetic alterations, to bacterial infections. Several plasmonic and silicon photonic-based biosensors are described together with their most recent applications in this area. We also identify and analyse the main challenges faced when attempting to harness this technology and how several innovative approaches introduced in the last years manage those issues, including the use of new biorecognition probes, surface functionalization approaches, signal amplification and enhancement strategies, as well as, sophisticated microfluidic solutions.
Ayudas:	Ministerio de Economía y Competitividad SEV-2017-0706 Ministerio de Economía y Competitividad BES-2017-080527 Ministerio de Economía y Competitividad TEC 2016-78515-R
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Biosensors ; Plasmonics ; Silicon photonics ; Microfluidics ; Nucleic acid analysis ; Epigenetics ; Surface chemistry ; Clinical diagnosis
Publicado en:	Frontiers in chemistry, Vol. 7 (October 2019) , art. 724, ISSN 2296-2646

DOI: 10.3389/fchem.2019.00724
PMID: 31709240

25 p, 1.7 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
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