Improved Alere Determine Lipoarabinomannan Antigen Detection Test for the Diagnosis of Human and Bovine Tuberculosis by Manipulating Urine and Milk
García, Juan Ignacio (Universitat Autònoma de Barcelona. Departament de Pediatria, d'Obstetrícia i Ginecologia i de Medicina Preventiva)
Kelley, Holden V. (Texas Biomedical Research Institute)
Meléndez, Johanna (Roosevelt Hospital)
Álvarez de León, Rosa Alejandra (Roosevelt Hospital)
Castillo, Alejandra (Clinica de Atención Integral Dr. Isaac Cohen Alcahe. Hospital de Especialidad Dr. Robles)
Sidiki, Sabeen (Ohio State University)
Yusoof, Kizil A. (Texas Biomedical Research Institute)
Nunes, Elizabete (Hospital Central de Maputo. Division of Pulmonology)
López Téllez, César (Clinica de Atención Integral Dr. Isaac Cohen Alcahe. Hospital de Especialidad Dr. Robles)
Mejía Villatoro, Carlos Rodolfo (Roosevelt Hospital)
Ikeda, Janet (Investigación, Desarrollo y Educación Integral (Quetzaltenango, Guatemala))
García-Basteiro, Alberto L.. (Amsterdam Institute for Global Health and Development)
Wang, Shu Hua (Ohio State University)
Torrelles, Jordi B. (Texas Biomedical Research Institute)

Date:	2019
Abstract:	Tuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with α-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 9 Núm. 1 (january 2019) , p. 18012, ISSN 2045-2322

DOI: 10.1038/s41598-019-54537-9
PMID: 31784649

6 p, 1.4 MB

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