Transcriptomic analysis of reproductive damage in the epididymis of male Kunming mice induced by chronic infection of Toxoplasma gondii PRU strain
Zheng, Yu-Xiang (Key Laboratory of Zoonosis Prevention and Control of Guangdong Province)
Zhang, Xiu-Xiang (South China Agricultural University. College of Plant)
Hernandez, Jorge A. (University of Florida. College of Veterinary Medicine)
Mahmmod, Yasser (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Huang, Wan Yi (Key Laboratory of Zoonosis Prevention and Control of Guangdong Province)
Li, Gui-Feng (Key Laboratory of Zoonosis Prevention and Control of Guangdong Province)
Wang, Ya-Pei. (Key Laboratory of Zoonosis Prevention and Control of Guangdong Province)
Zhou, Xue (Key Laboratory of Zoonosis Prevention and Control of Guangdong Province)
Li, Xiu-Ming (Heilongjiang BaYi Agricultural University. College of Animal Science and Technology)
Yuan, Zi-Guo (Key Laboratory of Zoonosis Prevention and Control of Guangdong Province)

Fecha:	2019
Resumen:	According to results of a bioinformatics analysis, Gene Ontology (GO) function is divided into three categories: Cellular component, molecular function and biological process. Upon performing GO analysis, we found that some DEGs correlated with an integral part of membrane, protein complex, cell surface, ATP binding, immune system process, signal transduction and metabolic process which are responsible for the epididymal injury. DEGs were mapped to 101 unique KEGG pathways. Pathways such as cytokine-cytokine receptor interaction, glycolysis/gluconeogenesis and apoptosis are closely related to sperm quality. Moreover, Tnfsf10 and spata18 can damage the mitochondria in sperm, which decreases sperm motility and morphology. Conclusions: We sequenced the reproductive system of male mice chronically infected with T. gondii, which provides a new direction for research into male sterility caused by Toxoplasma infection. This work provides valuable information and a comprehensive database for future studies of the interaction between T. gondii infection and the male reproductive system.
Resumen:	Background: Some researchers have reported that Toxoplasma gondii can cause serious reproductive impairment in male animals. Specifically, T. gondii destroy the quality of sperm in the epididymis, which affects their sexual ability. However, among such studies, none have investigated the male reproductive transcriptome. Therefore, to investigate the relationship between T. gondii and sperm maturation, we infected mice with T. gondii prugniaud (PRU) strain and performed transcriptome sequencing of the epididymis. Results: Compared with the control group, 431 upregulated and 229 downregulated differentially expressed genes (DEGs) were found (P-value < 0. 05, false discovery rate (FDR) < 0. 05 and l og2 (fold change).
Nota:	This work was supported, in part, by grants from the National Key Research and Development Programme of China (2017YFD0501305), National Natural Science Foundation of China (31972707), Major Scientific Research Projects (Characteristic Innovation) of Guangdong Province (2017KTSCX018), the Key Research and Development Programme of Guangdong Province (2019B020218004), the Guangzhou Zoo Project (h2016141), the Natural Science Foundation of Guangdong Province (2019A1515011534, 2016A030313396), and Huizhou Science and Technology Projects (2015B040009001).
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Toxoplasmosi ; RNA-seq ; Chronic infection ; Reproductive impairment ; DEGs
Publicado en:	Parasites & vectors, Vol. 12 Núm. 1 (august 2019) , p. 529, ISSN 1756-3305

DOI: 10.1186/s13071-019-3783-2
PMID: 31703718

11 p, 1.6 MB

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