High prevalence and diversity of extended-spectrum β-lactamase and emergence of OXA-48 producing Enterobacterales in wildlife in Catalonia
Darwich Soliva, Laila (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Vidal, Anna (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Seminati, Chiara (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Albamonte Rodríguez, Andreu (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Casado, A. (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
López, F. (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Molina-López, R. A. (Centre de Fauna Salvatge de Torreferrussa (Santa Perpètua de la Mogoda, Catalunya))
Migura-Garcia, Lourdes (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Fecha:	2019
Resumen:	Most of the studies focused on antimicrobial resistance (AMR) performed in wildlife describe Escherichia coli as the principal indicator of the selective pressure. In the present study, several species of Enterobacterales with a large panel of cephalosporin resistant (CR) genes have been isolated from wildlife in Catalonia. A total of 307 wild animals were examined to determine the prevalence of CR enterobacteria, AMR phenotypes and the presence of common carbapenem and CR genes. The overall prevalence of CR-phenotype was 13% (40/ 307): 17. 3% in wild mammals (18/104) and 11. 5% in wild birds (22/191) (p<0. 01). Hedgehogs showed the highest prevalence (13. 5% of 104) of the mammal specimens, and raptors the highest in bird specimen (7. 3% of 191). Although CR E. coli was the most frequently isolated (45%), other CR- Enterobacterales like Klebsiella pneumoniae (20%), Citrobacter freundii (15%), Enterobacter cloacae (5%), Proteus mirabilis (5%), Providencia spp (5%) and Serratia marcescens (2. 5%) were also isolated. A high diversity of CR genes was identified among the isolates, with 50% yielding blaCMY-2, 23% blaSHV-12, 20% blaCMY-1 and 18% blaCTX-M-15. Additionally, resistance to carbapenems associated to OXA-48 gene was found. Most of the CR isolates, principally K. pneumoniae and C. freundii, were multiresistant with co-resistance to fluoroquinolones, tetracycline, sulphonamides and aminoglycosides. This study reports high prevalence of Enterobacterales harbouring a variety of CR genes and OXA-48 mediated-carbapenem resistance, all of them frequently associated to nosocomial human infections, for the first time in wild mammals and wild birds. Implementation of control measures to reduce the impact of anthropogenic pressure in the environment is urgently needed.
Nota:	The contract of LMG was supported by the Instituto Nacional de Investigaci?n y Tecnolog?a Agraria y Alimentaria (INIA) and the European Social Fund. AV was supported by a PIF grant from the Universitat Aut?noma de Barcelona. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Our grateful thanks to the Torreferrussa WRC staff. A.C. was student of the Master?s Degree in Zoonosis and One Health (UAB). The authors are also grateful to the Centres de Recerca de Catalunya (CERCA) Programme.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Enterobacteriàcies ; Animals salvatges -- Malalties ; Animals ; Animals, Wild ; Beta-lactamases ; Biodiversity ; Drug Resistance, Bacterial ; Gammaproteobacteria ; Spain
Publicado en:	PloS one, Vol. 14 Núm. 8 (2019) , p. e0210686, ISSN 1932-6203

DOI: 10.1371/journal.pone.0210686
PMID: 31381578

15 p, 1.2 MB

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