Non-lysosomal activation in macrophages of atlantic salmon (Salmo salar) after infection with piscirickettsia salmonis
Pérez-Stuardo, Diego (Universidad Mayor. Centro de Genómica y Bioinformática)
Morales-Reyes, Jonathan (Consorcio de Sanidad Acuícola)
Tapia, Sebastián (Universidad Mayor. Centro de Genómica y Bioinformática)
Ahumada, Diego E. (Universidad Mayor. Centro de Genómica y Bioinformática)
Espinoza, Allison (Universidad Mayor. Centro de Genómica y Bioinformática)
Soto-Herrera, Valentina (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Brianson, Bernardo (Universidad Mayor. Centro de Genómica y Bioinformática)
Ibaceta, Valentina (Universidad Mayor. Centro de Genómica y Bioinformática)
Sandino, Ana María (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Spencer, Eugenio (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Vallejos Vidal, Eva Carolina (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Reyes-López, Felipe E. (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Valdés, Jorge (Universidad Mayor. Centro de Genómica y Bioinformática)
Reyes-Cerpa, Sebastián (Universidad Mayor. Centro de Genómica y Bioinformática)

Fecha:	2019
Resumen:	Piscirickettsia salmonis is a facultative intracellular pathogen and etiological agent of the systemic disease salmonid rickettsial septicemia. It has been suggested that P. salmonis is able to survive in host macrophages, localized within a vacuole like-compartment which prevents lysosomal degradation. However, the relevant aspects of the pathogenesis of P. salmonis as the host modulation that allow its intracellular survival have been poorly characterized. In this study, we evaluated the role of lysosomes in the response to P. salmonis infection in macrophage-enriched cell cultures established from Atlantic salmon head kidneys. Bacterial infection was confirmed using confocal microscopy. A gentamicin protection assay was performed to recover intracellular bacteria and the 16S rDNA copy number was quantified through quantitative polymerase chain reaction in order to determine the replication of P. salmonis within macrophages. Lysosomal activity in Atlantic salmon macrophage-enriched cell cultures infected with P. salmonis was evaluated by analyzing the lysosomal pH and proteolytic ability through confocal microscopy. The results showed that P. salmonis can survive ≥120 h in Atlantic salmon macrophage-enriched cell cultures, accompanied by an increase in the detection of the 16S rDNA copy number/cell. The latter finding suggests that P. salmonis also replicates in Atlantic salmon macrophage-enriched cell cultures. Moreover, this bacterial survival and replication appears to be favored by a perturbation of the lysosomal degradation system. We observed a modulation in the total number of lysosomes and lysosomal acidification following infection with P. salmonis. Collectively, the results of this study showed that infection of Atlantic salmon macrophages with P. salmonis induced limited lysosomal response which may be associated with host immune evasion mechanisms of P. salmonis that have not been previously reported.
Nota:	Altres ajuts: FONDECYT/11150807, CORFO 13CTI-21527, 101205
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Atlantic salmon (Salmo salar) ; Piscirickettsiosis ; Immune evasion mechanisms ; Macrophages ; Lysosome ; Proteolytic activity
Publicado en:	Frontiers in immunology, Vol. 10 (March 2019) , art. 434, ISSN 1664-3224

DOI: 10.3389/fimmu.2019.00434
PMID: 30941123

11 p, 3.6 MB

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