Neutron-irradiated antibody-functionalised carbon nanocapsules for targeted cancer radiotherapy
Wang, Julie T. W. (King's College London. Institute of Pharmaceutical Science)
Spinato, Cinzia (Centre National de la Recherche Scientifique (França))
Klippstein, Rebecca (King's College London. Institute of Pharmaceutical Science)
Costa, Pedro M. (King's College London. Institute of Pharmaceutical Science)
Martincic, Markus (Institut de Ciència de Materials de Barcelona)
Pach, Elzbieta (Institut Català de Nanociència i Nanotecnologia)
Pérez Ruiz De Garibay, Aritz (Centre National de la Recherche Scientifique (França))
Ménard-Moyon, Cécilia (Centre National de la Recherche Scientifique (França))
Feldman, Robert (Cis Bio International Ion Beam Applications SA)
Michel, Yves (Cis Bio International Ion Beam Applications SA)
Šefl, Martin (University of Ioannina Medical School)
Kyriakou, Ioanna (University of Ioannina Medical School)
Emfietzoglou, Dimitris (University of Ioannina Medical School)
Saccavini, Jean-Claude (Cis Bio International Ion Beam Applications SA)
Ballesteros, Belén (Institut Català de Nanociència i Nanotecnologia)
Tobias, Gerard (Institut de Ciència de Materials de Barcelona)
Bianco, Alberto (Centre National de la Recherche Scientifique (França))
Al-Jamal, Khuloud T. (King's College London. Institute of Pharmaceutical Science)

Fecha:	2020
Resumen:	Radiotherapy is a cancer treatment utilising high doses of ionizing radiation to destroy cancer cells. Our team has pioneered neutron activation of Sm, filled and sealed into single-walled (SWCNTs) and multi-walled carbon nanotubes (MWCNTs), to create stable and high-dose radioactive carbon nanocapsules for cancer radiotherapy. In this work, MWCNTs filled with enriched SmCl (Sm@MWCNTs) were sealed and irradiated, followed by surface functionalisation with an epidermal growth factor receptor (EGFR)-targeting antibody. Characterisation of functionalised Sm@MWCNTs was carried out using thermogravimetric analysis, gel electrophoresis and transmission electron microscopy. The organ biodistribution of the radioactive functionalised Sm@MWCNTs and therapeutic efficacy were studied in an experimental melanoma lung metastatic tumour model in mice after intravenous injection. Quantitative biodistribution analyses showed high accumulation of Sm@MWCNT-Ab in lung. Significant tumour growth reduction was induced by both treatments of Sm@MWCNTs functionalised with or without the antibody after a single intravenous injection. Although EGFR targeting showed no improvement in therapeutic efficacy, reduced spleen toxicity and normal haematological profiles were obtained for both functionalised derivatives. The current study demonstrated the possibility of performing chemical functionalisation and antibody conjugation on radioactive nanocapsules post-irradiation for the preparation of targeted radiopharmaceuticals.
Ayudas:	Ministerio de Economía y Competitividad SEV-2015-0496 Ministerio de Economía y Competitividad SEV-2017-0706 European Commission 290023
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Antibody conjugation ; Cancer radiotherapies ; Chemical functionalisation ; Epidermal growth factor receptors ; Gel electrophoresis ; Intravenous injections ; Surface functionalisation ; Therapeutic efficacy
Publicado en:	Carbon, Vol. 162 (June 2020) , p. 410-422, ISSN 0008-6223

DOI: 10.1016/j.carbon.2020.02.060

Postprint 35 p, 1.9 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
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