Large-scale production of adeno-associated viral vectors for human gene therapy : Alzheimer's disease. Part II: Upstream and Bioreaction
García Trujillo, Marc
Valero Barranco, Francisco, dir. (Universitat Autònoma de Barcelona)
Universitat Autònoma de Barcelona. Facultat de Biociències

Date:	2020
Abstract:	Currently, one of the main bottlenecks in the development of many human gene therapies is the lack of large-scale production methods of the required vectors. Owing to this fact, this work focuses on the development of an adeno-associated viral vector production process, specifically serotype 9 (AAV9), using suspension human embryonic kidney cells (HEK293) for the treatment of Alzheimer's disease. The proposed production process, based on perfusion culture in a rocking motion bioreactor, proves to be a robust method, achieving high yields greater than 2·10^5 vg/cell in serum-free conditions and without the need to use helper virus due to the triple plasmid transfection method using polyethylenimine (PEI).
Note:	Els autors del les altres parts del treball són Bentan, I. i Camacho A.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Studies:	Biotecnologia [2500253]
Study plan:	Grau en Biotecnologia [815]
Document:	Treball final de grau ; Text
Subject:	Human embryonic kidney cells (HEK293) ; Transfection ; Polyethylenimine (pei) ; Perfusion ; Adeno-associated viral vector (aav)

1 p, 3.0 MB

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Research literature > Bachelor's degree final project > Biosciences Faculty