Optimization of CASA-Mot Analysis of Donkey Sperm : optimum Frame Rate and Values of Kinematic Variables for Different Counting Chamber and Fields
Gacem, Sabrina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Soler, Carles (Universitat de València. Departament de Biologia Cel·lular, Biologia Funcional i Antropologia Física)
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Catalán, Jaime (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Valverde Abarca, Anthony (Costa Rica Institute of Technology. School of Agronomy)

Date:	2020
Abstract:	In order to optimize the donkey sperm motility analysis by the CASA (Computer Assisted Sperm Analysis)-Mot system, twelve ejaculates were collected from six jackasses. Capillary loaded chamber (CLC), ISAS®D4C depths 10 and 20 µm, ISAS®D4C Leja 20 and drop displacement chamber (DDC), Spermtrack® (Spk) depths 10 and 20 µm were used. Sperm kinematic variables were evaluated using each chamber and a high-resolution camera capable of capturing a maximum of 500 frames/second (fps). The optimum frame rate (OFR) (defined according to curvilinear velocity-VCL) was dependent on chamber type. The highest OFR obtained was 278. 46 fps by Spk20. Values for VCL, straight-line velocity (VSL), straightness (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were high in DDC and 10 µm depth. In both DDC 10 and 20 µm, the sperm velocities (VCL, VSL, VAP) and ALH values decreased significantly from the centre to the edges, while Wobble and BCF increased. No defined behavior was observed along the CLC. However, all the kinematic variables had a higher value in a highly concentrated sample, in both chamber types. In conclusion, analyzing a minimum of nine fields at 250 fps from the centre to the edges in Spk10 chamber using a dilution of 30 × 106 sperm/mL offers the best choice for donkey computerised sperm motility analysis.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Ases ; Esperma ; Frame rate ; Drop displacement chambers ; Capillary loaded chambers ; Chamber depth ; Field ; Sperm dilution
Published in:	Animals, Vol. 10 Núm. 11 (2020) , p. 1993, ISSN 2076-2615

DOI: 10.3390/ani10111993
PMID: 33138237

16 p, 1.4 MB

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